{"id":14766,"date":"2018-03-09T09:01:36","date_gmt":"2018-03-09T09:01:36","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/deteccion-rapida-de-actividad-mutagenica-en-procariotas-por-impedancia-electrica\/"},"modified":"2018-03-09T09:01:36","modified_gmt":"2018-03-09T09:01:36","slug":"deteccion-rapida-de-actividad-mutagenica-en-procariotas-por-impedancia-electrica","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-medicas\/deteccion-rapida-de-actividad-mutagenica-en-procariotas-por-impedancia-electrica\/","title":{"rendered":"Detecci\u00f3n r\u00e1pida de actividad mutag\u00e9nica en procariotas por impedancia el\u00e9ctrica"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Laura Garc\u00eda Pe\u00f1alver <\/strong><\/h2>\n<p>El objetivo principal de este estudio consisti\u00f3 en el desarrollo de un nuevo m\u00e9todo de an\u00e1lisis de actividad mutag\u00e9nica basado en el test de ames,utilizando para ello distintas cepas de salmonella typhimurium y m\u00e9todos impedim\u00e9tricos, en los que se detectar\u00e1 actividad microbina mediante mediciones de la impedancia el\u00e9ctrica. A lo largo de este trabajo se analizar\u00e1n distintos aspectos fundamentales para una adecuada puesta a punto del nuevo m\u00e9todo de mutagenicidad propuesto. Entre estos aspectos se contempla la obtenci\u00f3n y optimizaci\u00f3n del medio de cultivo a emplear, el protocolo de an\u00e1lisis, la realizaci\u00f3n posterior de an\u00e1lisis frente a distintos compuestos mutag\u00e9nicos directos, mut\u00e1genos indirectos, as\u00ed como frente a compuestos t\u00f3xicos e inocuos. Como m\u00e9todo de comparaci\u00f3n y poder as\u00ed determinar la sensibilidad y precisi\u00f3n de este nuevo m\u00e9todo, se realizar\u00e1n tambi\u00e9n ensayos empleando el m\u00e9todo tradicional de vertido en placa o test de ames. El test  de ames permite la detecci\u00f3n de actividad mutag\u00e9nica de un compuesto por reversi\u00f3n en placa de cepas derivadas de salmonella typhimurium y su empleo est\u00e1 recomendado por la uni\u00f3n europea y la organizaci\u00f3n para la cooperaci\u00f3n y desarrollo econ\u00f3mico.  a partir de este estudio se ha logrado desarrollar un medio de cultivo as\u00ed como un protocolo de an\u00e1lisis adecuado para la realizacion del test de mutagenicidad para la cepa de s. Typhimurium ta 100, empleando como unidad de medida la impedancia el\u00e9ctrica y  como instrumento de medici\u00f3n el bactometer. Este nuevo m\u00e9todo de detecci\u00f3n de actividad mutag\u00e9ncia propuesto, permiti\u00f3 clasificar adecudamente los mut\u00e1genos directos analizados: azida s\u00f3dica, nitrito s\u00f3dico, metanosulfonato de metilo, 2-nitrofluoreno, 4-nitro-1,2-fenilendiamina, 4-nitroquinole\u00edn-n-\u00f3xido, mostrando la misma sensibilidad y una mayor precisi\u00f3n (reflejada en la obtenci\u00f3n de menores coeficientes de variaci\u00f3n puntual) que el perfecta<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Detecci\u00f3n r\u00e1pida de actividad mutag\u00e9nica en procariotas por impedancia el\u00e9ctrica<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Detecci\u00f3n r\u00e1pida de actividad mutag\u00e9nica en procariotas por impedancia el\u00e9ctrica <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Laura Garc\u00eda Pe\u00f1alver <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Santiago de compostela<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 19\/12\/2001<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li> Sueiro Benavides Rosa Ana<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jes\u00fas Simal lozano <\/li>\n<li>tom\u00e1s Gonz\u00e1lez villa (vocal)<\/li>\n<li>Manuel l. Sanmart\u00edn dur\u00e1n (vocal)<\/li>\n<li>Mar\u00eda cristina Arias fern\u00e1ndez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Laura Garc\u00eda Pe\u00f1alver El objetivo principal de este estudio consisti\u00f3 en el desarrollo de un nuevo m\u00e9todo [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[8901,35,9,962,978,278,156,977,401],"tags":[2583,47397,2729,21382,35234,2553],"class_list":["post-14766","post","type-post","status-publish","format-standard","hentry","category-carcinogenesis","category-ciencias-de-la-vida","category-ciencias-medicas","category-genetica","category-metabolismo-microbiano","category-microbiologia","category-patologia","category-santiago-de-compostela","category-toxicologia","tag-jesus-simal-lozano","tag-laura-garcia-penalver","tag-manuel-l-sanmartin-duran","tag-maria-cristina-arias-fernandez","tag-sueiro-benavides-rosa-ana","tag-tomas-gonzalez-villa"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/14766","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=14766"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/14766\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=14766"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=14766"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=14766"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}