{"id":14900,"date":"2018-03-09T09:01:48","date_gmt":"2018-03-09T09:01:48","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/produccion-in-vitro-de-embriones-caprinos-sistemas-de-maduracion-citoplasmatica-de-ovocitos-de-hembras-prepuberes\/"},"modified":"2018-03-09T09:01:48","modified_gmt":"2018-03-09T09:01:48","slug":"produccion-in-vitro-de-embriones-caprinos-sistemas-de-maduracion-citoplasmatica-de-ovocitos-de-hembras-prepuberes","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-agrarias\/produccion-in-vitro-de-embriones-caprinos-sistemas-de-maduracion-citoplasmatica-de-ovocitos-de-hembras-prepuberes\/","title":{"rendered":"Producci\u00f3n in vitro de embriones caprinos: sistemas de maduraci\u00f3n citoplasm\u00e1tica de ovocitos de hembras prep\u00faberes"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Elisabet Rodr\u00edguez Gonz\u00e1lez <\/strong><\/h2>\n<p>La producci\u00f3n in vitro (piv) de embriones caprinos mediante t\u00e9cnicas de maduraci\u00f3n, fecundaci\u00f3n y cultivo embrionario in vitro (miv-fiv-civ) proporciona un elevado n\u00famero de embriones utilizables a nivel cient\u00edfico y\/o comercial.  el objetivo del estudio ha sido seleccionar ovocitos de cabra prepuber y sistemas de maduraci\u00f3n in vitro para mejorar la producci\u00f3n in vitro de embriones. Los ovocitos se maduraron en medio tcm199 + piruvato s\u00f3dico + l-glutamina + gentamicina + 10% suero bovino de machos castrado (ss) + 10 ug\/ml lh + 10 ug\/ml o-fsh + 1 ug\/ml 17beta-estradiol. Tras 27h de miv, los ovocitos se inseminaron con semen fresco (3.5&#215;10 espermatozoides\/ml) en medio talp suplementado con 1 ug\/ml de hipotaurina. A las 24 h post-inseminaci\u00f3n, se cultivaron en medio sofm y se mantuvieron 7 d\u00edas en cultivo.  en el estudio 1, se evalu\u00f3 la utilidad del test de azul de cresol brillante (bcb) para seleccionar los ovocitos de cabras prep\u00faberes. Los ovocitos bcb+ (ovocitos con citoplasma coloreado de azul, crecimiento finalizado) presentaron un mayor di\u00e1metro (136.6 +- 6.3 um; p&lt;0,001) y mayores tasas de maduraci\u00f3n nuclear (81.4%; p&lt;0,05), fecundaci\u00f3n normal (23.5%; p&lt;0,0001) y desarrollo m\u00e1s all\u00e1 del estadio de 8 c\u00e9lulas (41.3%; p&lt;0,001) y de m\u00f3rulas m\u00e1s blastocistos (12.00%; p&lt;0,05) que los ovocitos no coloreados de azul.  en el estudio 2, se utiliz\u00f3 el factor de crecimiento epid\u00e9mico (egf), para la maduraci\u00f3n in vitro. Para ello, se compar\u00f3 un medio suplementado con hormonas (lh, fsh, 17beta-estradiol) y suero (ss o fcs) con un medio sumplementado con 10 ng de egf en ausencia de suero y hormonas. El porcentaje de ovocitos fecundados de forma normal fue inferior (p&lt;0,05) cuando se emple\u00f3 egf (17.3%) que ss (ss: 27.2%; fcs: 29.0%). Las tasas de embriones totales (71.5%) y de embriones que superaron el estadio de 8 c\u00e9lulas (26.2%) fueron superiores (p&lt;0.05) al emplear ss y hormonas en el medio de mad<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Producci\u00f3n in vitro de embriones caprinos: sistemas de maduraci\u00f3n citoplasm\u00e1tica de ovocitos de hembras prep\u00faberes<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Producci\u00f3n in vitro de embriones caprinos: sistemas de maduraci\u00f3n citoplasm\u00e1tica de ovocitos de hembras prep\u00faberes <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Elisabet Rodr\u00edguez Gonz\u00e1lez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de barcelona<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 20\/12\/2001<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li> Paramio Nieto M. Teresa<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: francesca Vidal <\/li>\n<li>Fernando L\u00f3pez g\u00e1tius (vocal)<\/li>\n<li>bego\u00f1a Aguilar (vocal)<\/li>\n<li>pedro Lorenzo (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Elisabet Rodr\u00edguez Gonz\u00e1lez La producci\u00f3n in vitro (piv) de embriones caprinos mediante t\u00e9cnicas de maduraci\u00f3n, fecundaci\u00f3n y [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[494,1944,1946,3275],"tags":[47760,47758,47759,37063,29339,47761],"class_list":["post-14900","post","type-post","status-publish","format-standard","hentry","category-ciencias-agrarias","category-ovinos","category-produccion-animal","category-reproduccion-animal","tag-begona-aguilar","tag-elisabet-rodriguez-gonzalez","tag-fernando-lopez-gatius","tag-francesca-vidal","tag-paramio-nieto-m-teresa","tag-pedro-lorenzo"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/14900","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=14900"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/14900\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=14900"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=14900"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=14900"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}