{"id":15030,"date":"2002-09-01T00:00:00","date_gmt":"2002-09-01T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/analisis-funcional-del-autoanta%c2%adgeno-cenp-a-en-la-organizacion-estructural-del-centromero-de-mama%c2%adferos\/"},"modified":"2002-09-01T00:00:00","modified_gmt":"2002-09-01T00:00:00","slug":"analisis-funcional-del-autoanta%c2%adgeno-cenp-a-en-la-organizacion-estructural-del-centromero-de-mama%c2%adferos","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/analisis-funcional-del-autoanta%c2%adgeno-cenp-a-en-la-organizacion-estructural-del-centromero-de-mama%c2%adferos\/","title":{"rendered":"An\u00e1lisis funcional del autoant\u00edgeno cenp-a en la organizaci\u00f3n estructural del centr\u00f3mero de mam\u00edferos"},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Figueroa Garc\u00eda Francisco Javier <\/strong><\/h2>\n<p>El objetivo principal de este trabajo era profundizar en la funci\u00f3n que desempe\u00f1a el ant\u00edgeno centrom\u00e9rico humano cenp-a, en el papel general del centr\u00f3mero. Para ello se plantearon cinco objetivos puntuales a desarrollar:  1,- generar anticuerpos monoespec\u00edficos contra el ant\u00edgeno centrom\u00e9rico humano cenp-a para su uso posterior en ensayos tanto in vivo como in vitro.  2,- ensayos del papel de la prote\u00edna centrom\u00e9rica cenp-a durante distintas fases del ciclo celular mediante el an\u00e1lisis por microinyecci\u00f3n celular de los anticuerpos espec\u00edficos anti-cenp-a generados.  3,- establecer un protocolo inicial de purificaci\u00f3n del autoant\u00edgeno humano cenp-a a partir de c\u00e9lulas y\/o tejidos humanos, para su empleo en ensayos funcionales.  4,- realizar ensayos de inmunoprecipitaci\u00f3n de cromatina a partir de extractos nucleares humanos y analizar los componentes (\u00e1cidos nucleicos y prote\u00ednas) centrom\u00e9ricos coinmunoprecipitados con cenp-a.  5,- aislar, clonar y caracterizar el gen del autoant\u00edgeno cenp-a en el genoma de h\u00e1mster, y comparar la homolog\u00eda con el de otras especies ya descritas.  se ha generado un anticuerpo espec\u00edfico frente a un p\u00e9ptido de la regi\u00f3n aminoterminal de la prote\u00edna humana cenp-a. Dicho anticuerpo ha sido purificado en una columna de afinidad y posteriormente utilizado en ensayos de microinyecci\u00f3n en c\u00e9lulas humanas (hela), los cuales han revelado el papel esencial de cenp-a para la formaci\u00f3n del cinetocoro y la divisi\u00f3n de la c\u00e9lula.  posteriormente se ha establecido un protocolo de purificaci\u00f3n de la prote\u00edna nativa a partir de extractos de placenta humana, mediante solubilizaci\u00f3n de cromatina, cromatograf\u00eda de intercambio i\u00f3nico y de afinidad.  asimismo, se ha llevado a cabo la inmunoprecipitaci\u00f3n de complejos de cromatina centrom\u00e9rica con el mismo anticuerpo generado anteriormente. el an\u00e1lisis mediante secuenciaci\u00f3n de los adn inmunoprecipitados ha revelado que no presenta<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>An\u00e1lisis funcional del autoant\u00edgeno cenp-a en la organizaci\u00f3n estructural del centr\u00f3mero de mam\u00edferos<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 An\u00e1lisis funcional del autoant\u00edgeno cenp-a en la organizaci\u00f3n estructural del centr\u00f3mero de mam\u00edferos <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Figueroa Garc\u00eda Francisco Javier <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 C\u00e1diz<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 09\/01\/2002<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li> Martinez Valdivia Manuel J.<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jes\u00fas Avila de grado <\/li>\n<li>Juan Jim\u00e9nez mart\u00ednez (vocal)<\/li>\n<li>alfredo Villasante atienza (vocal)<\/li>\n<li>marina Garc\u00eda delgado (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Figueroa Garc\u00eda Francisco Javier El objetivo principal de este trabajo era profundizar en la funci\u00f3n que desempe\u00f1a [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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