{"id":15611,"date":"2018-03-09T09:02:51","date_gmt":"2018-03-09T09:02:51","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/proteccion-del-proceso-de-muerte-celular-programada-en-cultivos-in-vitro-de-celulas-animales\/"},"modified":"2018-03-09T09:02:51","modified_gmt":"2018-03-09T09:02:51","slug":"proteccion-del-proceso-de-muerte-celular-programada-en-cultivos-in-vitro-de-celulas-animales","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/proteccion-del-proceso-de-muerte-celular-programada-en-cultivos-in-vitro-de-celulas-animales\/","title":{"rendered":"Protecci\u00f3n del proceso de muerte celular programada en cultivos in vitro de c\u00e9lulas animales"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Joaquim Vives Armengol <\/strong><\/h2>\n<p>Ante la imposibilidad econ\u00f3mica y f\u00edsica de controlar ingenierilmente todos los par\u00e1metros involucrados en la p\u00e9rdida de viabilidad de los cultivos in vitro de c\u00e9lulas animales ya sea debido a limitaci\u00f3n en el subministro de nutrientes, as\u00ed como por la presencia de elevadas concentraciones de subproductos t\u00f3xicos del metabolismo, en este trabajo se ha explorado tanto la modificaci\u00f3n de la formulaci\u00f3n del medio de cultivo como gen\u00e9tica de c\u00e9lulas de hibridoma con el objetivo de prolongar la supervivencia dentro de los bioreactores y rendibilizar el proceso de obtenci\u00f3n de anticuerpos monoclonales.  la reformulaci\u00f3n del medio permiti\u00f3 relantizar la proliferaci\u00f3n celular y retardar el agotamiento de nutrientes esenciales. De todas formas, estos cambios favorecieron la inducci\u00f3n de la muerte celular por apoptosis.  el bloqueo de la apoptosis mediante el uso de inhibidores pept\u00eddicos espec\u00edficos de caspasas permiti\u00f3 no s\u00f3lo retardar la p\u00e9rdida de viabilidad de los cultivos si no tambi\u00e9n recuperarlos 36 horas despu\u00e9s de haber inducido la muerte por falta de glutamina.  resultados similares se obtuvieron en la inhibici\u00f3n gen\u00e9tica de la activaci\u00f3n de la ruta bioqu\u00edmica de las caspasas mediante la sobreexpresi\u00f3n de genes protectores de la familia de bcl-2, tanto de origen end\u00f3geno como v\u00edrico. adem\u00e1s, los cultivos de estas nuevas l\u00edneas celulares de hibridoma pueden ser recuperadas despu\u00e9s de haber sido sometidas hasta 48 horas bajo condiciones inductoras de la apoptosis.  estos resultados demuestran la viabilidad de la incorporaci\u00f3n de una v\u00e1lbula de seguridad gen\u00e9tica que permite retardar el proceso de muerte por apoptosis de c\u00e9lulas que, en otras circunstancias, morir\u00edan y se acumular\u00edan en el bioreactor. Adem\u00e1s, la sobreexpressi\u00f3n de genes antiapopt\u00f3ticos hace a las c\u00e9lulas m\u00e1s resistentes y permite recuperar cultivos que, por causas accidentales inherentes a la metodolog\u00eda utilizada en el monit<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Protecci\u00f3n del proceso de muerte celular programada en cultivos in vitro de c\u00e9lulas animales<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Protecci\u00f3n del proceso de muerte celular programada en cultivos in vitro de c\u00e9lulas animales <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Joaquim Vives Armengol <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de barcelona<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 15\/02\/2002<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Luis Cornudella Mir<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: francesc Godia casablancas <\/li>\n<li>jacint Boix torras (vocal)<\/li>\n<li>Jos\u00e9 vicente Castells ripoll (vocal)<\/li>\n<li>\u00ed\u00a0ngels Sierra jim\u00e9nez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Joaquim Vives Armengol Ante la imposibilidad econ\u00f3mica y f\u00edsica de controlar ingenierilmente todos los par\u00e1metros involucrados en [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[849,160,115,35,9399,17],"tags":[14192,49713,49712,49711,10754,29216],"class_list":["post-15611","post","type-post","status-publish","format-standard","hentry","category-biologia-celular","category-bioquimica","category-bioquimica-molecular","category-ciencias-de-la-vida","category-cultivo-celular","category-quimica","tag-francesc-godia-casablancas","tag-i-ngels-sierra-jimenez","tag-jacint-boix-torras","tag-joaquim-vives-armengol","tag-jose-vicente-castells-ripoll","tag-luis-cornudella-mir"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/15611","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=15611"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/15611\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=15611"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=15611"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=15611"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}