{"id":15777,"date":"2018-03-09T09:03:05","date_gmt":"2018-03-09T09:03:05","guid":{"rendered":""},"modified":"2018-03-09T09:03:05","modified_gmt":"2018-03-09T09:03:05","slug":"estudio-de-la-regulacion-de-la-ruta-de-degradacion-del-acido-3-hidroxifenilpropionico-en-escherichia-coli-y-sus-aplicaciones-para-el-desarrollo-de-nuevos-sistemas-de-contencion-activa-de-microorganism","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/estudio-de-la-regulacion-de-la-ruta-de-degradacion-del-acido-3-hidroxifenilpropionico-en-escherichia-coli-y-sus-aplicaciones-para-el-desarrollo-de-nuevos-sistemas-de-contencion-activa-de-microorganism\/","title":{"rendered":"Estudio de la regulaci\u00f3n de la ruta de degradaci\u00f3n del \u00e1cido 3-hidroxifenilpropi\u00f3nico en escherichia coli y sus aplicaciones para el desarrollo de nuevos sistemas de contenci\u00f3n activa de microorganismos"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Bego\u00f1a Torres Belinchon <\/strong><\/h2>\n<p>En esta tesis se han estudiado los elementos reguladores de la transcripci\u00f3n del \u00abcluster\u00bb mhp de escherichia coli para la degradaci\u00f3n del \u00e1cido 3-hidroxifenilpropi\u00f3nico. se han descrito los dos promotores divergentes de esta ruta, pa y pr. El activador espec\u00edfico de esta ruta, mhpr, es una prote\u00edna capaz de unirse in vitro al promotor pa. Los compuestos arom\u00e1ticos capaces de inducir la ruta mhp son 3-hidroxifenilpropi\u00f3nico (3 hpp), 3-hidroxicin\u00e1mico (3hci) y 3-dihidroxifenilpropi\u00f3nico (dhpp). La activaci\u00f3n del promotor pa esta sometida a represi\u00f3n catab\u00f3lica por glucosa mediada por el complejo crp-ampc. el producto del gen mhpt, la prote\u00edna mhpt, es un transportador de 3hpp en escherichia coli.  se ha demostrado en esta tesis que el sistema de metilaci\u00f3n-restricci\u00f3n ecori es un sistema id\u00f3neo para dise\u00f1ar circuitos de contenci\u00f3n gen\u00e9tica en proteobacterias y mediante la combinaci\u00f3n de este sistema con los elementos reguladores mhpr\/pa de la ruta de degradaci\u00f3n del 3hpp, se ha desarrollado el primer circuito de contenci\u00f3n biol\u00f3gica basado en un sistema de toxina\/ant\u00eddoto que responde a la presencia\/ausencia de 3hpp. Adem\u00e1s, se ha dise\u00f1ado por primera vez un circuito dual basado en la combinaci\u00f3n de funciones letales distintas (endonucleasa ecori y colicina e3), con dianas celulares diferentes (adn y arnr) y bajo controles independientes y se ha demostrado que este circuito reduce significativamente la frecuencia de transferencia de un gen marcador flanqueado por las dos funciones letales.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Estudio de la regulaci\u00f3n de la ruta de degradaci\u00f3n del \u00e1cido 3-hidroxifenilpropi\u00f3nico en escherichia coli y sus aplicaciones para el desarrollo de nuevos sistemas de contenci\u00f3n activa de microorganismos<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Estudio de la regulaci\u00f3n de la ruta de degradaci\u00f3n del \u00e1cido 3-hidroxifenilpropi\u00f3nico en escherichia coli y sus aplicaciones para el desarrollo de nuevos sistemas de contenci\u00f3n activa de microorganismos <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Bego\u00f1a Torres Belinchon <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 25\/02\/2002<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Eduardo D\u00edaz Fern\u00e1ndez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: ricardo Amils pibernat <\/li>\n<li>Juli\u00e1n Pereira gonz\u00e1lez (vocal)<\/li>\n<li>victor De lorenzo prieto (vocal)<\/li>\n<li>manolo Espinosa padr\u00f3n (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Bego\u00f1a Torres Belinchon En esta tesis se han estudiado los elementos reguladores de la transcripci\u00f3n del \u00abcluster\u00bb [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[36483,36606,35],"tags":[50159,48755,50160,50161,14786,4203],"class_list":["post-15777","post","type-post","status-publish","format-standard","hentry","category-biologia-molecular","category-biologia-molecular-de-microorganismos","category-ciencias-de-la-vida","tag-begona-torres-belinchon","tag-eduardo-diaz-fernandez","tag-julian-pereira-gonzalez","tag-manolo-espinosa-padron","tag-ricardo-amils-pibernat","tag-victor-de-lorenzo-prieto"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/15777","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=15777"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/15777\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=15777"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=15777"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=15777"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}