{"id":16724,"date":"2002-06-05T00:00:00","date_gmt":"2002-06-05T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-molecular-del-gen-de-la-beta-lactamasa-shv-1-en-klebsiella-pneumoniae\/"},"modified":"2002-06-05T00:00:00","modified_gmt":"2002-06-05T00:00:00","slug":"caracterizacion-molecular-del-gen-de-la-beta-lactamasa-shv-1-en-klebsiella-pneumoniae","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/caracterizacion-molecular-del-gen-de-la-beta-lactamasa-shv-1-en-klebsiella-pneumoniae\/","title":{"rendered":"Caracterizaci\u00f3n molecular del gen de la beta-lactamasa shv-1 en klebsiella pneumoniae"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Jose Chaves Puertas <\/strong><\/h2>\n<p>La naturaleza gen\u00e9tica del gen de la beta-lactamasar shv-1 ha sido analizada en 97 cepas de klebsiella pneumoniae procedentes de productos patol\u00f3gicos humanos y no relacionadas epidemiol\u00f3gicamente. Mediante ieea, se detectaron las bandas de pi 7,6 (shv-1) en 74 cepas, 7,1 (len-1) en 13 cepas y 5,4 (tem-1) en 10 cepas.  entre las 74 cepas shc-1, 40 mostraron una doble banda, 20 con pi 7,1 y 20 con pi 5,4. La mayor\u00eda de las 74 cepas shv-1 presentaron pl\u00e1smidos (76,7%). La transferencia por conjugaci\u00f3n de la beta-lactamasa shv-1 s\u00f3lo fue posible en 5 casos (9,3%). Se realiz\u00f3 una pcr-rflp shv-1 espec\u00edfica del  dna cromos\u00f3mico que result\u00f3 positiva para 93 de las 97 cepas y fue negativa \u00fanicamente en 4 cepas productoras de tem-1. Tambi\u00e9n se analiz\u00f3 el dna cromos\u00f3mico de las 97 cepas, en un intento de aproximaci\u00f3n al locus g\u00e9nico de shv-1 mediante restricci\u00f3n por endonucleasas. (Rflp) y southern-blot. la disgesti\u00f3n con ecori mostr\u00f3 al menos una banda positiva de hibridaci\u00f3n en 96 cepas, 2 bandas fueron detectadas en 8 muestras. \u00faNicamente en 1 cepa productoras de la beta-lactamasas tem-1 la hibridaci\u00f3n fue negativa. el an\u00e1lisis de las secuencias de dna no mostr\u00f3 diferencias en las regiones promotoras del gen, ni secuencia de fin de la transcripci\u00f3n adicionales; \u00fanicamente mutaciones puntuales que determinaron variantes al\u00e9licas. Se describieron algunas nuevas variantes que representaron cambios en la secuencia de amino\u00e1cidos de la prote\u00edna (shv-27, 28, 32 y 33 y la len-2). Como resultado de los estudios de southern-blot y secuenciaci\u00f3n podemos postular que shv-1 y len-1 est\u00e1n situados muy cercanos y en t\u00e1ndem en el cromosoma bacteriano de k.Pneumoniae. Nuestros resultados apoyan la hip\u00f3tesis de que el ancestro de shv-1 fue originado en el cromosoma bacteriano de k.Pneumoniae.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizaci\u00f3n molecular del gen de la beta-lactamasa shv-1 en klebsiella pneumoniae<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizaci\u00f3n molecular del gen de la beta-lactamasa shv-1 en klebsiella pneumoniae <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Jose Chaves Puertas <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Barcelona<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 06\/05\/2002<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Concepci\u00f3n Segura \u00e1lvarez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Miguel Regue <\/li>\n<li>jordi Vila (vocal)<\/li>\n<li>Luis Martinez (vocal)<\/li>\n<li> De la torre Rafael (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Jose Chaves Puertas La naturaleza gen\u00e9tica del gen de la beta-lactamasar shv-1 ha sido analizada en 97 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[1418,951,36483,36606,160,115,35,278,17],"tags":[52696,52699,49434,52695,52698,52697],"class_list":["post-16724","post","type-post","status-publish","format-standard","hentry","category-bacteriologia","category-barcelona","category-biologia-molecular","category-biologia-molecular-de-microorganismos","category-bioquimica","category-bioquimica-molecular","category-ciencias-de-la-vida","category-microbiologia","category-quimica","tag-concepcion-segura-alvarez","tag-de-la-torre-rafael","tag-jordi-vila","tag-jose-chaves-puertas","tag-luis-Martinez","tag-miguel-regue"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/16724","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=16724"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/16724\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=16724"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=16724"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=16724"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}