{"id":17278,"date":"2002-07-06T00:00:00","date_gmt":"2002-07-06T00:00:00","guid":{"rendered":""},"modified":"2002-07-06T00:00:00","modified_gmt":"2002-07-06T00:00:00","slug":"identificacion-de-los-danos-producidos-por-la-congelacion-seminal-en-la-membrana-plasmatica-de-espermatozoides-de-trucha-arco-iris-oncorhynchus-mykiss-y-optimizacion-de-los-protocolos-de-criopreserv","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/identificacion-de-los-danos-producidos-por-la-congelacion-seminal-en-la-membrana-plasmatica-de-espermatozoides-de-trucha-arco-iris-oncorhynchus-mykiss-y-optimizacion-de-los-protocolos-de-criopreserv\/","title":{"rendered":"Identificaci\u00f3n de los da\u00f1os producidos por la congelaci\u00f3n seminal en la membrana plasm\u00e1tica de espermatozoides de trucha arco iris (oncorhynchus mykiss) y optimizaci\u00f3n de los protocolos de criopreservaci\u00f3n"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Elsa Alexandra Cabrita <\/strong><\/h2>\n<p>El desarrollo de t\u00e9cnicas de cripreservaci\u00f3n seminal espec\u00edficas es un objetivo de indudable inter\u00e9s en acuicultura. No obstante, a pesar de los esfuerzos realizados, a\u00fan no se ha conseguido desarrollar m\u00e9todos de congelaci\u00f3n que permitan obtener tasas de fecundidad lo suficientemente elevadas como para permitir su empleo a escala comercial. Con estos objetivos, se han desarrollado tres series de experimentos. En la primera serie se trat\u00f3 de evaluar diferentes caracter\u00edsticas de la membrana plasm\u00e1tica antes y despu\u00e9s de la congelaci\u00f3n\/descongelaci\u00f3n. Las caracter\u00edsticas analizadas fueron: la resistencia de la membrana al estr\u00e9s osm\u00f3tico, la composici\u00f3n lip\u00eddica de la membrana del espermatozoide y la capacidad fusog\u00e9nica de los espermatozoides. Para el an\u00e1lisis de la respuesta al estr\u00e9s osm\u00f3tico se analiz\u00f3 mediante citometr\u00eda de flujo, la permeabilidad al ioduro de propidio de espermatozoides expuestos a medios de distinta osmolaridad. los resultados mostraron que la congelaci\u00f3n provoca una importante disminuci\u00f3n de la resistencia de los espermatozoides al estr\u00e9s osm\u00f3tico. En el segundo experimento, se pretendi\u00f3 determinar los efectos sobre la componente lip\u00eddica de la membrana plasm\u00e1tica, colesterol y fosfol\u00edpidos. Se analiz\u00f3 la proporci\u00f3n cho\/pl en semen fresco, diluido y criopreservado. La capacidad fusog\u00e9nica de la membrana plasm\u00e1tica se analiz\u00f3 evaluando la fusi\u00f3n de los espermatozoides, frescos, diluidos y criopreservados con liposomas marcados con fluorescencia. ninguno de los m\u00e9todos ensayados consigui\u00f3 una activaci\u00f3n significativa de la capacidad fusog\u00e9nica del espermatozoide, demostrando que los mecanismos de reconocimiento y fusi\u00f3n con el ovocito son, en trucha, diferentes a los descritos en mam\u00edferos. La diluci\u00f3n en el medio de congelaci\u00f3n y, especialmente la cripreservaci\u00f3n, inducen una p\u00e9rdida del control de la capacidad fusog\u00e9nica de los espermatozoides, ya que un elevado porcen<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Identificaci\u00f3n de los da\u00f1os producidos por la congelaci\u00f3n seminal en la membrana plasm\u00e1tica de espermatozoides de trucha arco iris (oncorhynchus mykiss) y optimizaci\u00f3n de los protocolos de criopreservaci\u00f3n<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Identificaci\u00f3n de los da\u00f1os producidos por la congelaci\u00f3n seminal en la membrana plasm\u00e1tica de espermatozoides de trucha arco iris (oncorhynchus mykiss) y optimizaci\u00f3n de los protocolos de criopreservaci\u00f3n <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Elsa Alexandra Cabrita <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Le\u00f3n<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 07\/06\/2002<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Paz Herraez Ortega<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jos\u00e9 julian Garde lopez-brea <\/li>\n<li>teresa Abaigar anc\u00edn (vocal)<\/li>\n<li> Peleteiro alonso Jos\u00e9 benito (vocal)<\/li>\n<li>Manuel Carrillo est\u00e9vez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Elsa Alexandra Cabrita El desarrollo de t\u00e9cnicas de cripreservaci\u00f3n seminal espec\u00edficas es un objetivo de indudable inter\u00e9s [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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