{"id":17522,"date":"2018-03-09T09:05:38","date_gmt":"2018-03-09T09:05:38","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/transportadores-de-albumina-para-la-administracion-de-oligonucleotidos-antisentido\/"},"modified":"2018-03-09T09:05:38","modified_gmt":"2018-03-09T09:05:38","slug":"transportadores-de-albumina-para-la-administracion-de-oligonucleotidos-antisentido","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-medicas\/transportadores-de-albumina-para-la-administracion-de-oligonucleotidos-antisentido\/","title":{"rendered":"Transportadores de alb\u00famina para la administraci\u00f3n de oligonucle\u00f3tidos antisentido"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Amaia Arnedo Hern\u00e1ndez <\/strong><\/h2>\n<p>Los oligonucle\u00f3tidos antisentido son mol\u00e9culas terap\u00e9uticas altamente espec\u00edficas para el tratamiento de enfermedades virales, enfermedades autoinmunes y c\u00e1ncer entre otras. Sin embargo su aplicaci\u00f3n terap\u00e9utica est\u00e1 limitada por su baja estabilidad enzim\u00e1tica, su incapacidad para atravesar la membrana celular, y su r\u00e1pida degradaci\u00f3n en endosomas. En vista de las propiedades fusog\u00e9nicas de la alb\u00famina y su afinidad de uni\u00f3n por los oligonucle\u00f3tidos se han desarrollado dos sistemas transportadores de oligonucle\u00f3tidos basados en alb\u00famina: nanopart\u00edculas y oligonucle\u00f3tidos, la cuantificaci\u00f3n de los oligonucle\u00f3tidos se ha realizado por electroforesis capilar en zona que nos permite cuantificar los antisentidos con precisi\u00f3n y exactitud, de forma r\u00e1pida y sencilla. Los complejos alb\u00famina-oligonucle\u00f3tido se han caracterizado mediante espectroscop\u00eda de fluorescencia y anisotrop\u00eda de fluorescencia, obteniendo una estequiometr\u00eda de entre 4 y 5 mol\u00e9culas de oligonucle\u00f3tido interaccionan con cada mol\u00e9cula de oligonucle\u00f3tido, con una constante de afinidad cercana a 10-8. Los estudios de asociaci\u00f3n celular mostraron una diminuci\u00f3n de las asociaci\u00f3n de oligonucle\u00f3tido a c\u00e9lulas en presencia de alb\u00famina, adem\u00e1s los complejos modificaron el patr\u00f3n de internalizaci\u00f3n citoplasm\u00e1tico perinuclear hacia una elevada localizaci\u00f3n nuclear. Finalmente se realizaron estudios de degradaci\u00f3n enzim\u00e1tica de los oligonucle\u00f3tidos, comprobado que los complejos no eran capaces de proteger a los oligonucle\u00f3tidos de su degradaci\u00f3n. Finalmente se ha estudiado la capacidad de los complejos para incrementar la actividad antiviral de los oligonucle\u00f3tidos mediante dos ensayos, reducci\u00f3n en placa y beta-galactosidasa, ambos ensayos mostraron que los complejos aumentaron la actividad de los oligonucle\u00f3tidos libres.  se han desarrollado dos sistemas nanoparticulares mediante desolvataci\u00f3n controlada en etanol y posterior reticulac<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Transportadores de alb\u00famina para la administraci\u00f3n de oligonucle\u00f3tidos antisentido<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Transportadores de alb\u00famina para la administraci\u00f3n de oligonucle\u00f3tidos antisentido <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Amaia Arnedo Hern\u00e1ndez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Navarra<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 17\/06\/2002<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Juan  Manuel Irache Garreta<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Miguel S\u00e1nchez gonz\u00e1lez <\/li>\n<li>Manuel Guzm\u00e1n navarro (vocal)<\/li>\n<li> Alonso fern\u00e1ndez m. Jos\u00e9 (vocal)<\/li>\n<li> Go\u00f1i leza m. mar (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Amaia Arnedo Hern\u00e1ndez Los oligonucle\u00f3tidos antisentido son mol\u00e9culas terap\u00e9uticas altamente espec\u00edficas para el tratamiento de enfermedades virales, [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[9,2445,957,17749],"tags":[3219,54816,32130,43303,3223,18204],"class_list":["post-17522","post","type-post","status-publish","format-standard","hentry","category-ciencias-medicas","category-evaluacion-de-farmacos","category-farmacologia","category-navarra","tag-alonso-fernandez-m-jose","tag-amaia-arnedo-hernandez","tag-goni-leza-m-mar","tag-juan-manuel-irache-garreta","tag-manuel-guzman-navarro","tag-miguel-sanchez-gonzalez"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/17522","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=17522"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/17522\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=17522"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=17522"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=17522"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}