{"id":17778,"date":"2018-03-09T09:06:01","date_gmt":"2018-03-09T09:06:01","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/purificacion-de-enzimas-nadh-dependientes-de-pediococcus-acidilactici-mediante-colorantes-textiles-y-ciclodextrinas\/"},"modified":"2018-03-09T09:06:01","modified_gmt":"2018-03-09T09:06:01","slug":"purificacion-de-enzimas-nadh-dependientes-de-pediococcus-acidilactici-mediante-colorantes-textiles-y-ciclodextrinas","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/purificacion-de-enzimas-nadh-dependientes-de-pediococcus-acidilactici-mediante-colorantes-textiles-y-ciclodextrinas\/","title":{"rendered":"Purificaci\u00f3n de enzimas nadh-dependientes de pediococcus acidilactici mediante colorantes textiles y ciclodextrinas."},"content":{"rendered":"<h2>Tesis doctoral de <strong>  L\u00f3pez M\u00e1s Jos\u00e9 Aniceto <\/strong><\/h2>\n<p>La bacteria del \u00e1cido l\u00e1ctico pediococcus acidilactici crecida aer\u00f3bicamente en un medio conteniendo glicerol como carbohidrato fermetable result\u00f3 ser una buena de enzimas nadh-dependientes, permitiendo la obtenci\u00f3n de las enzimas modelo en el estudio de las interacciones entre prote\u00ednas y colorantes reactivos, lactato deshidrogenasa y nadh-oxidasa. La combinaci\u00f3n de dos columnas de afinidad en tandem y de distintas estrategias de eluci\u00f3n inespec\u00edficas, con etilenglicol, y espec\u00edficas, con nadh, permite desarrollar un m\u00e9todo de purificaci\u00f3n en un solo paso para el caso de la l-ldh, obteniendo una enzima purificada en 58 veces con un 85% de recuperaci\u00f3n, en dos pasos para el caso de la nadh-peroxidasa, con una purificaci\u00f3n de 146 veces y un 59% de recuperaci\u00f3n, y en tres pasos para el caso de la nadh-oxidasa (nox-2), con una purificaci\u00f3n de 18 veces y un 41% de recuperaci\u00f3n.  la nadh-peroxidasa de pediococcus acidilactici puede obtenerse pura combinando la cromatograf\u00eda de afinidad en tandem con una columna de intercambio ani\u00f3nico, obteniendo un grado de purificaci\u00f3n tres veces superior al citado en la bibliograf\u00eda para la nadh-peroxidasa de enterococcus faecalis, manteni\u00e9ndose su porcentaje de recuperaci\u00f3n. As\u00ed mismo, se reduce el tiempo de purificaci\u00f3n a un tercio, de tres d\u00edas a un solo d\u00eda.  la l-ldh de pediococcus acidilactici, purificada sobre una columna de procion red mx-5b, presenta una gran especificidad por sus sustratos naturales, piruvato, l-lactato, nadh y nad+, obteni\u00e9ndose para todos ellos curvas de saturaci\u00f3n michaelianas, confirm\u00e1ndose la naturaleza no alost\u00e9rica de esta enzima. Los par\u00e1metros cin\u00e9ticos calculados sugieren fuertemente que el papel principal de esta l-ldh in vivo es la reducci\u00f3n de piruvato con la consiguiente reoxidaci\u00f3n de nadh. Esta deshidrogenasa es fuertemente inhibida por numerosos colorantes textiles compitiendo \u00e9stos con el nadh.  durante esta tesis se ha desarr<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Purificaci\u00f3n de enzimas nadh-dependientes de pediococcus acidilactici mediante colorantes textiles y ciclodextrinas.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Purificaci\u00f3n de enzimas nadh-dependientes de pediococcus acidilactici mediante colorantes textiles y ciclodextrinas. <\/li>\n<li><strong>Autor:<\/strong>\u00a0  L\u00f3pez M\u00e1s Jos\u00e9 Aniceto <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 26\/06\/2002<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Alvaro Sanchez Ferrer<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Francisco Garc\u00eda carmona <\/li>\n<li>Manuel Esteban morales (vocal)<\/li>\n<li> L\u00f3pez roca Jos\u00e9 Mar\u00eda (vocal)<\/li>\n<li>edelmira Valero ruiz (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de L\u00f3pez M\u00e1s Jos\u00e9 Aniceto La bacteria del \u00e1cido l\u00e1ctico pediococcus acidilactici crecida aer\u00f3bicamente en un medio conteniendo [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[160,955,8235,17],"tags":[55479,44407,5284,55478,2093,32306],"class_list":["post-17778","post","type-post","status-publish","format-standard","hentry","category-bioquimica","category-enzimologia","category-murcia","category-quimica","tag-alvaro-sanchez-ferrer","tag-edelmira-valero-ruiz","tag-francisco-garcia-carmona","tag-lopez-mas-jose-aniceto","tag-lopez-roca-jose-maria","tag-manuel-esteban-morales"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/17778","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=17778"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/17778\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=17778"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=17778"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=17778"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}