{"id":17831,"date":"2018-03-09T09:06:06","date_gmt":"2018-03-09T09:06:06","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/apoptosis-y-criopreservacion-de-progenitores-hematopoyeticos-de-sangre-periferica\/"},"modified":"2018-03-09T09:06:06","modified_gmt":"2018-03-09T09:06:06","slug":"apoptosis-y-criopreservacion-de-progenitores-hematopoyeticos-de-sangre-periferica","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/apoptosis-y-criopreservacion-de-progenitores-hematopoyeticos-de-sangre-periferica\/","title":{"rendered":"Apoptosis y criopreservaci\u00f3n de progenitores hematopoyeticos de sangre periferica"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Aranzazu Garc\u00eda Grande <\/strong><\/h2>\n<p>La cantidad de c\u00e9lulas cd34+ infundidas constituye un par\u00e1metro de gran valor para asegurar el \u00e9xito del implante del producto de c\u00e9lulas progenitoras hematopoy\u00e9ticas trasplantado. Su cuantificaci\u00f3n por citometria de flujo es considerado hoy el m\u00e9todo de elecci\u00f3n aceptado para la determinaci\u00f3n de phsp. Este trabajo propone el desarrollo de una t\u00e9cnica citom\u00e9trica nueva que incluye todas las permisas conocidas para la cuantificaci\u00f3n de c\u00e9lulas cd34+ en muestras de leucoaf\u00e9resis y que permite conocer adem\u00e1s, su viabilidad mediante identificaci\u00f3n y cuantificaci\u00f3n de las c\u00e9lulas cd34+ apopt\u00f3ticas y necr\u00f3ticas. Para comprobar la validez del m\u00e9todo propuesto, las determinaciones se realizaron en comparaci\u00f3n con dos m\u00e9todos ya estandarizados, y en dos momentos diferentes: antes de la congelaci\u00f3n y tras la descongelaci\u00f3n de los progenitores hematopoy\u00e9ticos. Adem\u00e1s, se estudia el efecto de la criopreservaci\u00f3n sobre las c\u00e9lulas mediante la incorporaci\u00f3n del marcador de detecci\u00f3n de apoptosis precoz anexina v.  los resultados obtenidos indican que con nuestro m\u00e9todo es mayor la poblaci\u00f3n de progenitores que se obtiene tanto en la precongelaci\u00f3n como tras la descongelaci\u00f3n. Ello es debido a la aplicaci\u00f3n del sistema de ventanas consecutivas, la utilizaci\u00f3n de fluoresfera que permite la cuantificaci\u00f3n de la concentraci\u00f3n celular, y a la utilizaci\u00f3n de fluorocromos que ofrecen marcaje m\u00e1s espec\u00edfico y mayor avidez por el ant\u00edgeno cd34. En cuanto al estudio del efecto de la cripreservaci\u00f3n se detecta disminuci\u00f3n de la concentraci\u00f3n de progenitores tras la descongelaci\u00f3n. Estas variaciones sin embargo no parecen estar relacionadas con p\u00e9rdidas selectiva de c\u00e9lulas sino con la disminuci\u00f3n en la inmunorreactividad por alteraci\u00f3n del ant\u00edgeno cd34.  finalmente, el estudio de otros factores como la utilizaci\u00f3n de determinada quimioterapia en el tratamiento previo, el uso de quimioterapia en la movilizaci\u00f3n<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Apoptosis y criopreservaci\u00f3n de progenitores hematopoyeticos de sangre periferica<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Apoptosis y criopreservaci\u00f3n de progenitores hematopoyeticos de sangre periferica <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Aranzazu Garc\u00eda Grande <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 27\/06\/2002<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Fernando Hern\u00e1ndez Navarro<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Juan  jos\u00e9 V\u00e1zquez rodr\u00edguez <\/li>\n<li>Alberto De matas (vocal)<\/li>\n<li>Ana Villegas mart\u00ednez (vocal)<\/li>\n<li>Antonio Escudero soto (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Aranzazu Garc\u00eda Grande La cantidad de c\u00e9lulas cd34+ infundidas constituye un par\u00e1metro de gran valor para asegurar [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[35,1737,2953],"tags":[55619,9707,54898,55618,8245,4860],"class_list":["post-17831","post","type-post","status-publish","format-standard","hentry","category-ciencias-de-la-vida","category-inmunologia","category-trasplante-de-organos","tag-alberto-de-matas","tag-ana-villegas-Martinez","tag-antonio-escudero-soto","tag-aranzazu-garcia-grande","tag-fernando-hernandez-navarro","tag-juan-jose-vazquez-rodriguez"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/17831","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=17831"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/17831\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=17831"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=17831"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=17831"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}