{"id":17871,"date":"2018-03-09T09:06:09","date_gmt":"2018-03-09T09:06:09","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/analisis-estructura-funcion-de-prfa-el-regulador-central-de-la-virulencia-de-listeria\/"},"modified":"2018-03-09T09:06:09","modified_gmt":"2018-03-09T09:06:09","slug":"analisis-estructura-funcion-de-prfa-el-regulador-central-de-la-virulencia-de-listeria","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-agrarias\/analisis-estructura-funcion-de-prfa-el-regulador-central-de-la-virulencia-de-listeria\/","title":{"rendered":"An\u00e1lisis estructura-funci\u00f3n de prfa, el regulador central de la virulencia de listeria"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Yolanda Vega Rocha <\/strong><\/h2>\n<p>La mayor parte de los genes de virulencia descritos hasta el momento en listeria monocytogenes est\u00e1n sometidos a una regulaci\u00f3n positiva coordinada a trav\u00e9s de la prote\u00edna prfa, un factor de transcripci\u00f3n que presenta homolog\u00edas estructurales con la prote\u00edna receptora de ampc de escherichia coli, m\u00e1s conocidad como crp o cap. En este trabajo nos hemos aproximado al estudio estructura-funci\u00f3n de prfa a trav\u00e9s de la identificaci\u00f3n y an\u00e1lisis de mutaciones que afectan a la actividad de esta importante prote\u00edna de l.Monocytogenes, esencial para la virulencia y la patog\u00e9nesis del proceso infeccioso. Hemos identificado dos nuevas mutaciones prfa*, ile45ser y leu140phe, que al igual que la mutaci\u00f3n gly14ser previamente descrita, resultan en la expresi\u00f3n constitutiva de elevados niveles de factores prfa-dependientes en condiciones normalmente represoras para las cepas silvestres de l.Monocytogenes. Estas sustituciones se localizan en regiones de prfa con un alto grado de conservaci\u00f3n respecto de las regiones hom\u00f3logas de crp y en las que mapean mutaciones de naturaleza y efectos similares. Por analog\u00eda con crp y su mecanismos alost\u00e9rico de activaci\u00f3n por ampc, estas mutaciones mimetizar\u00edan en prfa los cambios conformacionales inducidos por la uni\u00f3n de un hipot\u00e9tico cofactor activador, todav\u00eda por identificar, y que resultar\u00edan en la exposici\u00f3n del motivo h\u00e9lice-giro-h\u00e9lice responsable de su uni\u00f3n espec\u00edfica a la secuencia diana de dna. Adem\u00e1s de las mutaciones prfa*, hemos identificado a partir de una cepa de genotipo prfa* (gly145ser) una mutaci\u00f3n de prfa, glu173gly, id\u00e9ntica y localizada en la misma posici\u00f3n en las prote\u00ednas alineadas que una mutaci\u00f3n crp*sup (glu171gly) que anula el efecto activador de la sustituci\u00f3n crp* ala144thr (a su vez localizada en una posici\u00f3n alineada exactamente con la de la mutaci\u00f3n prfa* gly145ser). Todas estas observaciones constituyen una clara evidencia de que prfa no s\u00f3lo e<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>An\u00e1lisis estructura-funci\u00f3n de prfa, el regulador central de la virulencia de listeria<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 An\u00e1lisis estructura-funci\u00f3n de prfa, el regulador central de la virulencia de listeria <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Yolanda Vega Rocha <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Complutense de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 28\/06\/2002<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Jos\u00e9 Antonio Vazquez  Boland<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: guillermo Suarez fernandez <\/li>\n<li>klaus Brhem (vocal)<\/li>\n<li>Fernando Baquero mochales (vocal)<\/li>\n<li>Francisco Garcia del portillo (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Yolanda Vega Rocha La mayor parte de los genes de virulencia descritos hasta el momento en listeria [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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