{"id":18200,"date":"2002-08-07T00:00:00","date_gmt":"2002-08-07T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/efecto-del-plasma-seminal-y-de-las-espermadhesinas-sobre-espermatozoides-de-verraco\/"},"modified":"2002-08-07T00:00:00","modified_gmt":"2002-08-07T00:00:00","slug":"efecto-del-plasma-seminal-y-de-las-espermadhesinas-sobre-espermatozoides-de-verraco","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-agrarias\/efecto-del-plasma-seminal-y-de-las-espermadhesinas-sobre-espermatozoides-de-verraco\/","title":{"rendered":"Efecto del plasma seminal y de las espermadhesinas sobre espermatozoides de verraco"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Fernando Centuri\u00f3n Castro <\/strong><\/h2>\n<p>Este estudio se realiz\u00f3 con la finalidad de evaluar si el plasma seminal (ps) completo, fracciones del plasma seminal o prote\u00ednas presentes en el plasma seminal influyen sobre los espermatozoides de verracos en muestras diluidas. Se utilizaron eyaculados completos en la experiencia 1 mientras que en el resto de las experiencias solo se utiliz\u00f3 fracciones ricas del eyaculado. Cada muestra a procesar se dividi\u00f3 en dos al\u00edcuotas. La primera fue destinada a la obtenci\u00f3n de espermatozoides, diluy\u00e9ndolas en bts hasta una concentraci\u00f3n de 3 x 107 esper.\/Ml., Mientras que la segunda se utiliz\u00f3 para la obtenci\u00f3n de plasma seminal. El ps se extrajo del eyaculado por centrifugaci\u00f3n a 3.800 x g durante 15 minutos a 25\u00c2\u00bac. El sobrenadante se filtr\u00f3 con filtros de nylon de 30m y 10m de di\u00e1metro. Una vez filtrado el ps, o se utiliz\u00f3 en fresco, se almacen\u00f3 a -20\u00c2\u00bac o se liofiliz\u00f3. La fracci\u00f3n de prote\u00ednas que unen heparina (hbp), fue aislada a partir del ps completo por cromatograf\u00eda de afinidad sobre una columna de heparina-sepharosa descrita por sanz et al. (1993). La obtenci\u00f3n de las prote\u00ednas que no unen heparina (psp-i\/psp-ii) fueron aisladas por cromatografia de afinidad seg\u00fan calvete et al. (1995).  para la evaluaci\u00f3n de las caracter\u00edsticas esperm\u00e1ticas por efecto del ps, el eyaculado se diluy\u00f3 de nuevo hasta de 3 x 106 esp.\/Ml. La viabilildad fue evaluada con diacetato de carboxifluoresceina y yoduro de propidio, la actividad mitocondrial fue evaluada con rodamina 123 y, finalmente, la motilidad se evalu\u00f3 de forma objetiva utilizando un sistema de an\u00e1lisis por ordenador. En algunas experiencias se evalu\u00f3 la calidad de movimiento mediante el sistema sca. Los par\u00e1metros utilizados fueron la velocidad mediante la velocidad curvil\u00ednea (vcl), la linealidad (lin) y el dance. en la \u00faltima experiencia se evalu\u00f3 la capacidad fecundamente de los espermatozoides mediante el test hom\u00f3logo de penetraci\u00f3n descrito por mart\u00edne<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Efecto del plasma seminal y de las espermadhesinas sobre espermatozoides de verraco<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Efecto del plasma seminal y de las espermadhesinas sobre espermatozoides de verraco <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Fernando Centuri\u00f3n Castro <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 08\/07\/2002<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Juan  Mar\u00eda Vazquez Rojas<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: emilio Espinosa velazquez <\/li>\n<li>agust\u00edn Josa serrano (vocal)<\/li>\n<li>Luis Anel rodr\u00edguez (vocal)<\/li>\n<li>Juan  Jos\u00e9 Calvete chornet (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Fernando Centuri\u00f3n Castro Este estudio se realiz\u00f3 con la finalidad de evaluar si el plasma seminal (ps) [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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