{"id":18295,"date":"2002-11-07T00:00:00","date_gmt":"2002-11-07T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/control-de-la-sa%c2%adntesi-de-glicogen-en-hepata%c2%b2cits-en-cultiu\/"},"modified":"2002-11-07T00:00:00","modified_gmt":"2002-11-07T00:00:00","slug":"control-de-la-sa%c2%adntesi-de-glicogen-en-hepata%c2%b2cits-en-cultiu","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/control-de-la-sa%c2%adntesi-de-glicogen-en-hepata%c2%b2cits-en-cultiu\/","title":{"rendered":"Control de la s\u00edntesi de glicogen en hepat\u00f3cits en cultiu"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Roger Gomis Cabre <\/strong><\/h2>\n<p>1,- se ha construido un adenovirus recombinante que se dirige para la expresi\u00f3n de la gluc\u00f3geno sintasa (gs) de h\u00edgado de rata. Se ha usado \u00e9ste adenovirus y otro que codifica para la glucoquinasa (gk) para sobreexpresar dichas prote\u00ednas en cultivo primario de hepatocitos. La glucosa activa la gs sobreexpresada de un modo dependiente de la concentraci\u00f3n presente en el medio y causa grandes acumulaciones de gluc\u00f3geno en el caso de los hepatocitos tratados con el adenovirus adcmv-rlgs. La concentraci\u00f3n de metabolitos intermediarios de la v\u00eda glucogenog\u00e9nica, como son la glucosa 6-fosfato (glc 6-p) y el udp-glucosa, no presentan alteraciones significativas. La sobreexpresi\u00f3n de la gk tambi\u00e9n confiere a los hepatocitos una capacidad mayor de acumulaci\u00f3n de gluc\u00f3geno en respuesta a glucosa como ya se hab\u00eda descrito anteriormente, aunque en este caso, acumulan glc 6-p. Cuando se sobreexpresan la gs y la gk, la acumulaci\u00f3n de gluc\u00f3geno es mayor que en las c\u00e9lulas que sobreexpresan la gs o la gk. Nuestros resultados est\u00e1n de acuerdo con la hip\u00f3tesis que la gs de h\u00edgado cataliza el paso limitante en la s\u00edntesis de gluc\u00f3geno hep\u00e1tico. Aunque, la acumulaci\u00f3n de gluc\u00f3geno procedente de glucosa se encuentra sometida a un sistema de control compartido en el cual, el controlador, la gs, est\u00e1 controlado por la gk. Este sistema de control indirecto se realiza a trav\u00e9s de los niveles de glc 6-p, que encienden la desfosforilaci\u00f3n y activaci\u00f3n de la gs.  2,- se utilizaron adenovirus recombinantes para la transducci\u00f3n de genes en c\u00e9lulas fto-2b, una l\u00ednea celular de hepatoma de rata. Concretamente se sobreexpres\u00f3 hk i, gk, gs hep\u00e1tica (lgs), gs muscular (mgs) y combinaciones de cada una de las enzimas fosforiladoras de glucosa con cada una de las isoformas de gs. Las c\u00e9lulas fto-2b no sintetizan gluc\u00f3geno incluso cuando se incuban a elevadas concentraciones de glucosa. La sobreexpresi\u00f3n de la hk i y la lgs, dos enzimas exp<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Control de la s\u00edntesi de glicogen en hepat\u00f3cits en cultiu<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Control de la s\u00edntesi de glicogen en hepat\u00f3cits en cultiu <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Roger Gomis Cabre <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Barcelona<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 11\/07\/2002<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Joan Guinovart Cirera<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: ramon Bartrons bach <\/li>\n<li>loranne Agius (vocal)<\/li>\n<li>joaqu\u00edn Ari\u00f1o carmona (vocal)<\/li>\n<li>albert Barbera lLuis (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Roger Gomis Cabre 1,- se ha construido un adenovirus recombinante que se dirige para la expresi\u00f3n de [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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