{"id":18778,"date":"2018-03-09T09:07:28","date_gmt":"2018-03-09T09:07:28","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/analisis-fenotipico-linfocitario-y-del-ciclo-celular-en-biopsias-ganglionares-mediante-citometria-de-flujo\/"},"modified":"2018-03-09T09:07:28","modified_gmt":"2018-03-09T09:07:28","slug":"analisis-fenotipico-linfocitario-y-del-ciclo-celular-en-biopsias-ganglionares-mediante-citometria-de-flujo","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-medicas\/analisis-fenotipico-linfocitario-y-del-ciclo-celular-en-biopsias-ganglionares-mediante-citometria-de-flujo\/","title":{"rendered":"An\u00e1lisis fenotipico linfocitario y del ciclo celular en biopsias ganglionares mediante citometria de flujo"},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Mu\u00f1oz De Benito Rosa M. <\/strong><\/h2>\n<p>La citometr\u00eda de flujo es una t\u00e9cnica din\u00e1mica que permite realizar un an\u00e1lisis multiparam\u00e9trico de c\u00e9lulas en suspensi\u00f3n.  objetivos  realizar un an\u00e1lisis inmunofernot\u00edpico por c.F., De las subpoblaciones linfocitarias en biopsias ganglionares. Estudiar la ploid\u00eda y el ciclo celular. Analizar la apoptosis y la expresi\u00f3n de bc-2, p53 y fas. Estudio de la expresi\u00f3n del fenotipo mdr. Se han analizado 185 muestras (62 procesos benignos, 73 linfomas no-hodgkin (lnh), 28 enfermedad de hodgkin (eh) y 21 met\u00e1stasis ganglionares.  metodolog\u00eda  tras obtener la suspensi\u00f3n celular se incubaron las c\u00e9lulas con combinaciones de anticuerpos monoclonales (ac) marcados con fluorocromos \u00f3 con loduro de propidio (ip). Panel con las combinaciones de ac:  cd45-cd14-hladr; cd3-cd56\/cd4-cd8; cd20-k-*; cd19-k-*; cd19-cd20-cd5; cd19-cd20-cd-10; cd5-cd23-cd10; cd3-cd-25-hladr; cd3-alfa,beta-gamma*; cd3-cd4-cd8; cd45ra-cd45ro-cd4; cd45ra-cd45ro-cd8; cd15-cd30-cd45; cd16-cd56-cd3; cd3-cd95; cd19-cd95-cd5; mdr; bcl-2; p53; ip; anexina.  la adquisi\u00f3n se hizo en un cit\u00f3mero facsort con los programas lysis-ii y cell-fit y el an\u00e1lisis con paint-a-gate.  resultados  en muestras benignas la poblaci\u00f3n mayoritaria est\u00e1 constitu\u00edda por c\u00e9lula t no activada, predominando los linfocitos colaboradores v\u00edrgenes (cd4cd45ro). la mayor\u00eda de las c\u00e9lulas se hallan en fase g0-g1 del ciclo.  las met\u00e1stasis ganglionares se reconocen por la presencia de una poblaci\u00f3n que no expresa cd45. Se halla aumentada la poblaci\u00f3n de c\u00e9lula t activada cd3hla-dr y las subpoblaciones cd8 y cd3cd56. Se eleva el porcentaje de c\u00e9lulas en fase de s\u00edntesis. En eh: se observa un aumento de cd3cd25 y de la subpoblaci\u00f3n de linfocitos colaboradores de memoria cd4d45ro, elevandose el porcentaje de cd3-tcrgamma*.  los lnh-b fueron identificados por restricci\u00f3n de cadenas ligeras de superficie con una sensibilidad del 87% y por un predominio de la poblaci\u00f3n cd19. L<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>An\u00e1lisis fenotipico linfocitario y del ciclo celular en biopsias ganglionares mediante citometria de flujo<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 An\u00e1lisis fenotipico linfocitario y del ciclo celular en biopsias ganglionares mediante citometria de flujo <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Mu\u00f1oz De Benito Rosa M. <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 29\/07\/2002<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Alberto Durante Martinez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: melchor Alvarez de mon soto <\/li>\n<li>pilar Espa\u00f1a saz (vocal)<\/li>\n<li>julia Jorda tormo (vocal)<\/li>\n<li>Rafael Laguna mart\u00ednez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Mu\u00f1oz De Benito Rosa M. La citometr\u00eda de flujo es una t\u00e9cnica din\u00e1mica que permite realizar un [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[9,218,450,139,1027,156],"tags":[14289,17599,14431,58051,58052,14432],"class_list":["post-18778","post","type-post","status-publish","format-standard","hentry","category-ciencias-medicas","category-hematologia","category-inmunopatologia","category-medicina-interna","category-oncologia","category-patologia","tag-alberto-durante-Martinez","tag-julia-jorda-tormo","tag-melchor-alvarez-de-mon-soto","tag-munoz-de-benito-rosa-m","tag-pilar-espana-saz","tag-rafael-laguna-Martinez"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/18778","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=18778"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/18778\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=18778"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=18778"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=18778"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}