{"id":18966,"date":"2018-03-09T09:07:45","date_gmt":"2018-03-09T09:07:45","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracteritzacio-de-la-nucleoplasmina-de-x-laevis-i-la-seva-interaccio-amb-protea%c2%afnes-basiques\/"},"modified":"2018-03-09T09:07:45","modified_gmt":"2018-03-09T09:07:45","slug":"caracteritzacio-de-la-nucleoplasmina-de-x-laevis-i-la-seva-interaccio-amb-protea%c2%afnes-basiques","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/caracteritzacio-de-la-nucleoplasmina-de-x-laevis-i-la-seva-interaccio-amb-protea%c2%afnes-basiques\/","title":{"rendered":"Caracteritzaci\u00f3 de la nucleoplasmina de x.laevis i la seva interacci\u00f3 amb prote\u00c1\u00afnes b\u00e1siques"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Celia Prieto S\u00e1nchez <\/strong><\/h2>\n<p>La nucleoplasmina (np) es una prote\u00edna fosforilable, ac\u00eddica y termoestable que se encuentra en el n\u00facleo de los oocitos y huevos de xenopus laevis, donde es la prote\u00edna m\u00e1s abundante. Las dos funciones principales en las que est\u00e1 implicada la np son: el ensamblaje de nucleosomas y la descondensaci\u00f3n de la cromatina esperm\u00e1tica. A la hora de iniciar este trabajo se plante\u00f3 como objetivo principal obtener informaci\u00f3n estructural de la nucleoplasmina as\u00ed como de su interacci\u00f3n con prote\u00ednas b\u00e1sicas. En un principio se puso a punto la obtenci\u00f3n de np a partir de la disecci\u00f3n de ovarios de rana, pero m\u00e1s adelante se plante\u00f3 la opci\u00f3n de expresar la prote\u00edna en e.Coli. de esta manera se obtuvieron varios mutantes dise\u00f1ados estrat\u00e9gicamente para conseguir el m\u00e1ximo de informaci\u00f3n de la mol\u00e9cula. A partir de diferentes m\u00e9todos se obtuvo un grado de pureza adecuado de las formas expresadas; salvando el obst\u00e1culo que representa la formaci\u00f3n de unas prote\u00ednas de peso molecular inferior que han sido denominadas formas truncadas y cuya caracterizaci\u00f3n ha permitido concluir en que se dan durante la expresi\u00f3n de la np y en que se originan por el extremo carboxilo terminal. Mediante ensayos de ultracentr\u00edfuga anal\u00edtica y de dicro\u00edsmo circular se ha comprobado la validez de las diferentes prote\u00ednas recombinantes obtenidas en lo que a estructura secundaria y cuaternaria se refiere. A nivel funcional se han determinado diversas cuestiones relacionadas con la uni\u00f3n de la np a prote\u00ednas b\u00e1sicas. Los resultados obtenidos apuntan que el tramo ac\u00eddico m\u00e1s carboxilo terminal de la prote\u00edna no participa en la descondensaci\u00f3n de la cromatina esperm\u00e1tica; mientras que el tramo poliglut\u00e1mico principal de la mol\u00e9cula, aunque no es imprescindible, s\u00ed que es muy importante a la hora de realizar esta actividad, ya que su eliminaci\u00f3n reduce mucho la eficacia con que la np desplaza las protaminas del dna esperm\u00e1tico. la determ<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracteritzaci\u00f3 de la nucleoplasmina de x.laevis i la seva interacci\u00f3 amb prote\u00c1\u00afnes b\u00e1siques<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracteritzaci\u00f3 de la nucleoplasmina de x.laevis i la seva interacci\u00f3 amb prote\u00c1\u00afnes b\u00e1siques <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Celia Prieto S\u00e1nchez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Polit\u00e9cnica de catalunya<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 16\/09\/2002<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Nuria Saperas Plana<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Juan Aguilar piera <\/li>\n<li>manel Chiva royo (vocal)<\/li>\n<li>rosa Aligue alemany (vocal)<\/li>\n<li>arturo Muga villarte (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Celia Prieto S\u00e1nchez La nucleoplasmina (np) es una prote\u00edna fosforilable, ac\u00eddica y termoestable que se encuentra en [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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