{"id":19197,"date":"2018-03-09T09:08:06","date_gmt":"2018-03-09T09:08:06","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/clonacion-y-caracterizacion-del-anta%c2%adgeno-celular-porcino-cd47\/"},"modified":"2018-03-09T09:08:06","modified_gmt":"2018-03-09T09:08:06","slug":"clonacion-y-caracterizacion-del-anta%c2%adgeno-celular-porcino-cd47","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-agrarias\/clonacion-y-caracterizacion-del-anta%c2%adgeno-celular-porcino-cd47\/","title":{"rendered":"Clonaci\u00f3n y caracterizaci\u00f3n del ant\u00edgeno celular porcino cd47"},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Abdel-aziz Shahein Yasser Ezzat <\/strong><\/h2>\n<p>En esta tesis se presenta: la identificaci\u00f3n y clonaci\u00f3n del adnc que codifica para el hom\u00f3logo porcino de la prote\u00edna asociada a integrina (iap o cd47), la localizaci\u00f3n cromos\u00f3mica del gen correspondiente y la caracterizaci\u00f3n funcional de dicha mol\u00e9cula. Se gener\u00f3 una sonda molecular espec\u00edfica mediante amplificaci\u00f3n por pcr de adnc de lelucocitos porcinos, utilizando cebadores basados en regiones consensus entre las secuencias conocidas de cd47 procedentes de diferentes especies. El rastreo (screening) de una genoteca de adnc de m\u00fasculo liso de aorta de cerdo identific\u00f3 seite clones conteniendo todos ellos secuencias id\u00e9nticas. Los clones comprend\u00edan una fase abierta de lectura (orf) que codificaban un p\u00e9ptido se\u00f1al putativo de 18 amino\u00e1cidos, una secuencia de 122 amino\u00e1cidos correspondiente a un dominio extracelular de tipo inmunoglobulina variable (igv) seguida por una regi\u00f3n de 147 amino\u00e1cidos conteniendo cinco dominios que atraviesan la membrana y una cola citopl\u00e1smica de 16 amino\u00e1cidos. Dicha secuencia de amino\u00e1cidos presentaba un 73% de homolog\u00eda con la secuencia humana de iap, por lo que se la denomin\u00f3 iap porcina o cd47 porcino. La rt-pcr mostr\u00f3 que el cd47 porcino se expresa en una gran variedad de tejidos.  mediante citometr\u00eda de flujo, se ha mostrado que el anticuerpo monoclonal bric 126, anti-cd47 humano, reacciona tanto con plaquetas como con c\u00e9lulas ov\u00e1ricas de h\u00e1mster chino (c\u00e9lulas cho) transfectadas con el adnc que codifica para el cd47 porcino. El an\u00e1lisis por western-blot de cd47 de eritrocitos y plaquetas de cerdo revel\u00f3 un peso molecular de 43-50 kda y de 55-65 kda, respectivamente, en condiciones no reductoras. El cd47 porcino se expresaba de modo estable en las c\u00e9lulas cho transfectadas y se un\u00eda a trombospondina humana (tsp). El anticuerpo bric-126, anti-cd47 humano, inhib\u00eda la uni\u00f3n de plaquetas y de c\u00e9lulas transfectadas con cd47 a tsp humana y a fibrin\u00f3geno porcino, en ta<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Clonaci\u00f3n y caracterizaci\u00f3n del ant\u00edgeno celular porcino cd47<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Clonaci\u00f3n y caracterizaci\u00f3n del ant\u00edgeno celular porcino cd47 <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Abdel-aziz Shahein Yasser Ezzat <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 C\u00f3rdoba<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 27\/09\/2002<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li> De Andr\u00e9s Cara Dami\u00e1n Fermin<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: diego Llanes  ruiz <\/li>\n<li>angel L\u00f3pez carrascosa (vocal)<\/li>\n<li>Jos\u00e9 Antonio Barcena ruiz (vocal)<\/li>\n<li>karin Haverson (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Abdel-aziz Shahein Yasser Ezzat En esta tesis se presenta: la identificaci\u00f3n y clonaci\u00f3n del adnc que codifica [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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