{"id":19787,"date":"2002-06-11T00:00:00","date_gmt":"2002-06-11T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-de-los-enzimas-implicados-en-la-regulacion-de-la-funcion-senalizadora-de-los-diadenosina-polifosfatos\/"},"modified":"2002-06-11T00:00:00","modified_gmt":"2002-06-11T00:00:00","slug":"caracterizacion-de-los-enzimas-implicados-en-la-regulacion-de-la-funcion-senalizadora-de-los-diadenosina-polifosfatos","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/la-laguna\/caracterizacion-de-los-enzimas-implicados-en-la-regulacion-de-la-funcion-senalizadora-de-los-diadenosina-polifosfatos\/","title":{"rendered":"Caracterizaci\u00f3n de los enzimas implicados en la regulaci\u00f3n de la funci\u00f3n se\u00f1alizadora de los diadenosina polifosfatos"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Aaron Cabrera Asensio <\/strong><\/h2>\n<p>Los objetivos de esta tesis son:  1,- caracterizaci\u00f3n y purificaci\u00f3n de enzimas que degradan diadenosina polifosfatos intracelularmente (ap3aasa y ap4aasa) y de ectoenzimas que los degradan extracelularmente.  2,- comparaci\u00f3n de actividad ap3aasa citos\u00f3lica humana con la prote\u00edna supresora de tumores fhit.  para garantizar una caracterizaci\u00f3n exhaustiva de los enzimas investigados se utilizaron una amplia gama de t\u00e9cnicas: cromatograf\u00eda convencional y hplc, electroforesis, ensayos de fluorescencia, ensayos enzim\u00e1ticos, y diversos modelos experimentales: citosol de tejidos de rata, membranas de cerebro, cerebelo y sinaptosomas de rata, ovocitos de xenopus laevis y citosol de leucocitos y plaquetas humanos. Estos diferentes modelos permitieron la comparaci\u00f3n de las caracter\u00edsticas de los enzimas estudiados en diferentes organismos y sistemas.  los ensayos de la actividad enzim\u00e1tica se realizaron con eteno derivados de diadenosona polifosfatos como sustratos fluorog\u00e9nicos. Ello supone una gran simplificaci\u00f3n sobre t\u00e9cnicas radiom\u00e9tricas y cromatogr\u00e1ficas anteriormente en uso, y posibilita la valoraci\u00f3n de la actividad ap3aasa en cl\u00ednica mediante un sencillo, econ\u00f3mico y r\u00e1pido m\u00e9todo fluorim\u00e9trico.  los resultados indican que es imposible distinguer cin\u00e9ticamente entre la actividad ap3aasa citos\u00f3lica de plaquetas y leucocitos y la actividad ap3aasa de fhit, que la suramina es el inhibidor m\u00e1s potente descrito hasta el momento de la actividad ap3aasa y que el enzima ap3aasa presenta un masa molecular de 32 kda en condiciones nativas y de 17 kda en sds-page. se trata de la primera caracterizaci\u00f3n del enzima ap3a hidrolasa humano.  el estudio comparativo entre el enzima ap3a hidrolasa y el supresor tumoral fhit ha revelado que ambas prote\u00ednas son id\u00e9nticas o isoformas estrechamente relacionadas. Por primera vez se atribuye una funci\u00f3n espec\u00edfica al enzima ap3a hidrolasa a pesar del tiempo transcurrido desde<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizaci\u00f3n de los enzimas implicados en la regulaci\u00f3n de la funci\u00f3n se\u00f1alizadora de los diadenosina polifosfatos<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizaci\u00f3n de los enzimas implicados en la regulaci\u00f3n de la funci\u00f3n se\u00f1alizadora de los diadenosina polifosfatos <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Aaron Cabrera Asensio <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 La laguna<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 06\/11\/2002<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Pedro Rotlllan Pascual<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Mar\u00eda  teresa Miras portugal <\/li>\n<li> Corzo varillas Francisco Javier (vocal)<\/li>\n<li>Miguel \u00e1ngel Falc\u00f3n sanabria (vocal)<\/li>\n<li>enrique Castro l\u00f3pez-tarruella (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Aaron Cabrera Asensio Los objetivos de esta tesis son: 1,- caracterizaci\u00f3n y purificaci\u00f3n de enzimas que degradan [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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