{"id":19801,"date":"2002-07-11T00:00:00","date_gmt":"2002-07-11T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-de-dos-casea%c2%adna-quinasas-1-de-trypanosoma-cruzi\/"},"modified":"2002-07-11T00:00:00","modified_gmt":"2002-07-11T00:00:00","slug":"caracterizacion-de-dos-casea%c2%adna-quinasas-1-de-trypanosoma-cruzi","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/caracterizacion-de-dos-casea%c2%adna-quinasas-1-de-trypanosoma-cruzi\/","title":{"rendered":"Caracterizaci\u00f3n de dos case\u00edna quinasas 1 de trypanosoma cruzi"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Carmenza Spadafora Mej\u00eda <\/strong><\/h2>\n<p>Los objetivos de la tesis fueron:  1,- identificar, mediante el rastreo de una librer\u00eda de expresi\u00f3n, secuencias relacionadas con la familia de case\u00edna quinasas 1 en t.Cruzi.  2,- caracterizar molecularmente el gen de la ck1 de t.Cruzi para conocer su organizaci\u00f3n gen\u00f3mica y su expresi\u00f3n a lo largo del ciclo de vida del par\u00e1sito.  3,- desarrollar un sistema de expresi\u00f3n heter\u00f3logo de la enzima ck1 de t.Cruzi, e.Coli y purificar la enzima recombinante para caracterizar sus propiedades bioqu\u00edmicas.  4,- desarrollar un sistema de expresi\u00f3n hom\u00f3logo de la ck1 para estudiar el efecto de inhibidores de otras ck1 tanto en el crecimiento como en la infectividad de t.Cruzi.  siguiendo los objetivos planteados, y utilizando una sonda obtenida por pcr de la zona conservada de ck1, se aislaron dos secuencias cdna que codifican para dos isoformas ck1 en t.Cruzi. Una de las isoformas, tcck1.1, tiene 936 pb y la otra, tcck1.2, tiene 990 pb. Estas isoformas codifican para prote\u00ednas de 36 y 37 kda, respectivamente.  mediante el uso de sondas espec\u00edficas para cada una de las isoformas hibridas contra southern blots se pudo determinar que tcck1.1 es un gen de dos copias y tcck1.2 est\u00e1 multiplicado en t\u00e1ndem. La separaci\u00f3n de cromosomas por electroforesis de campo pulsado revel\u00f3 que ambos genes se localizan en dos cromosomas de m\u00e1s de 2 mb. El modelaje te\u00f3rico de la estructura de ambas isoformas revela diferencias con respecto a otras ck1 cristalizadas, en los residuos que hacen a la prote\u00edna funcional o interaccionan con ella. al hibridar nothern blots con las sondas espec\u00edficas para cada gen, se determin\u00f3 que ambas isoformas se expresan diferencialmente en los diferentes estad\u00edos de vida de t.Cruzi, presentando una mayor expresi\u00f3n (a la altura de arn) en el estad\u00edo infectivo, para tcck1.1, y en la forma intracelular amastigota, para tcck1.2. La expresi\u00f3n global de las ck1 en t.Cruzi revela niveles significativamente m\u00e1s e<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizaci\u00f3n de dos case\u00edna quinasas 1 de trypanosoma cruzi<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizaci\u00f3n de dos case\u00edna quinasas 1 de trypanosoma cruzi <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Carmenza Spadafora Mej\u00eda <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Granada<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 07\/11\/2002<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Santiago Castanys Cuello<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: alberto Vargas morales <\/li>\n<li>aurora Galvan cejudo (vocal)<\/li>\n<li>Luis Rivas l\u00f3pez (vocal)<\/li>\n<li>basilio Balladares hern\u00e1ndez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Carmenza Spadafora Mej\u00eda Los objetivos de la tesis fueron: 1,- identificar, mediante el rastreo de una librer\u00eda [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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