{"id":20047,"date":"2018-03-09T09:09:14","date_gmt":"2018-03-09T09:09:14","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/identificacion-de-nuevos-anta%c2%adgenos-para-el-diagnostico-serologico-de-la-leishmaniasis-visceral\/"},"modified":"2018-03-09T09:09:14","modified_gmt":"2018-03-09T09:09:14","slug":"identificacion-de-nuevos-anta%c2%adgenos-para-el-diagnostico-serologico-de-la-leishmaniasis-visceral","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/identificacion-de-nuevos-anta%c2%adgenos-para-el-diagnostico-serologico-de-la-leishmaniasis-visceral\/","title":{"rendered":"Identificaci\u00f3n de nuevos ant\u00edgenos para el diagn\u00f3stico serol\u00f3gico de la leishmaniasis visceral"},"content":{"rendered":"

Tesis doctoral de Mohammed El Bali <\/strong><\/h2>\n

Las leishmaniasis son un grupo de enfermedades causadas por un protozoo del g\u00e9nero leishmania, que afectan a m\u00e1s e 14 millones de personas, con 400.000 casos nuevos cada a\u00f1o, actualmente con tendencia a un fuerte incremento por su asociaci\u00f3n al sida. La leishmaniasis visceral (lv) es una forma extendida por muchas zonas, entre ellas la cuenta mediterr\u00e1nea d\u00f3nde se debe a l.Infantum y puede ser mortal sin tratamiento. las t\u00e9cnicas serol\u00f3gicas m\u00e1s comunmente aplicadas al diagn\u00f3stico de la lv son: la inmunofluorescencia indirecta, elisa, el western blot y el test de aglutinaci\u00f3n directa. Estos m\u00e9todos utilizan como ant\u00edgenos par\u00e1sitos completos o extractos solubles de los mismos que presentan problemas fundamentalmente de especificidad. Por ello nos propusimos identificar y caracterizar nuevos genes de l.Infantum que codifiquen prote\u00ednas antig\u00e9nicas de inter\u00e9s diagn\u00f3stico empleando herramientas de biolog\u00eda molecular y su validaci\u00f3n mediante elisa con sueros de enfermos con lv y otras patolog\u00edas que suelen presentar reacciones cruzadas con la leishmaniasis. el inmunocribado de una genoteca de expresi\u00f3n de l.Infantum nos permiti\u00f3 la identificaci\u00f3n de un gran n\u00famero de clones altamente inmunog\u00e9nicos, cuyos an\u00e1lisis selectivo mediante southern-blot y immuno-dot-blot permiti\u00f3 la elecci\u00f3n de seis nuevas mol\u00e9culas: dos mol\u00e9culas cuyas secuencias presentaron alta homolog\u00eda con la nucle\u00f3sido difosfato kinasa (ndpk) y la poli-ubiquitina (pubq), as\u00ed como cuatro nuevas mol\u00e9culas, n\u00fameradas arbitrariamente 75, 222, 229 y 241, que no presentaron homolog\u00edas significativas con las secuencias disponibles en los bancos de secuencias. Las mol\u00e9culas 75, 222 y 229 est\u00e1n formadas por secuencias repetidas con un alto \u00edndice de antigenicidad te\u00f3rico. los ant\u00edgenos recombinantes ndpk, pubq y 241, y la mezcla de dos p\u00e9ptidos sint\u00e9ticos, 75r1+222r1, sintetizados a partir de las secuencias repetidas de las mol\u00e9culas 75<\/p>\n

 <\/p>\n

Datos acad\u00e9micos de la tesis doctoral \u00abIdentificaci\u00f3n de nuevos ant\u00edgenos para el diagn\u00f3stico serol\u00f3gico de la leishmaniasis visceral<\/strong>\u00ab<\/h3>\n
    \n
  • T\u00edtulo de la tesis:<\/strong>\u00a0 Identificaci\u00f3n de nuevos ant\u00edgenos para el diagn\u00f3stico serol\u00f3gico de la leishmaniasis visceral <\/li>\n
  • Autor:<\/strong>\u00a0 Mohammed El Bali <\/li>\n
  • Universidad:<\/strong>\u00a0 Complutense de Madrid<\/li>\n
  • Fecha de lectura de la tesis:<\/strong>\u00a0 21\/11\/2002<\/li>\n<\/ul>\n

     <\/p>\n

    Direcci\u00f3n y tribunal<\/h3>\n
      \n
    • Director de la tesis<\/strong>\n
        \n
      • Jorge Alvar Ezquerra<\/li>\n<\/ul>\n<\/li>\n
      • Tribunal<\/strong>\n
          \n
        • Presidente del tribunal: Antonio ramon Martinez fernandez <\/li>\n
        • Jim\u00e9nez alonso Mar\u00eda isabel (vocal)<\/li>\n
        • Luis Rivas l\u00f3pez (vocal)<\/li>\n
        • leslie Chance michael (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n

           <\/p>\n","protected":false},"excerpt":{"rendered":"

          Tesis doctoral de Mohammed El Bali Las leishmaniasis son un grupo de enfermedades causadas por un protozoo del g\u00e9nero leishmania, […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[1],"tags":[664,61202,22232,61203,22320,61201],"class_list":["post-20047","post","type-post","status-publish","format-standard","hentry","category-sin-categoria","tag-antonio-ramon-Martinez-fernandez","tag-jimenez-alonso-maria-isabel","tag-jorge-alvar-ezquerra","tag-leslie-chance-michael","tag-luis-rivas-lopez","tag-mohammed-el-bali"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/20047","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=20047"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/20047\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=20047"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=20047"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=20047"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}