{"id":20355,"date":"2002-04-12T00:00:00","date_gmt":"2002-04-12T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/analisis-molecular-del-establecimiento-de-la-impronta-en-los-genes-u2af1-rs1-y-h19-en-la-la%c2%adnea-germinal-masculina\/"},"modified":"2002-04-12T00:00:00","modified_gmt":"2002-04-12T00:00:00","slug":"analisis-molecular-del-establecimiento-de-la-impronta-en-los-genes-u2af1-rs1-y-h19-en-la-la%c2%adnea-germinal-masculina","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/pais-vasco-euskal-herriko-unibertsitatea\/analisis-molecular-del-establecimiento-de-la-impronta-en-los-genes-u2af1-rs1-y-h19-en-la-la%c2%adnea-germinal-masculina\/","title":{"rendered":"An\u00e1lisis molecular del establecimiento de la impronta en los genes u2af1-rs1 y h19 en la l\u00ednea germinal masculina"},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Zalduendo Macua M. Mar <\/strong><\/h2>\n<p>En el presente trabajo se ha analizado el patr\u00f3n de metilaci\u00f3n y la estructura de la cromatina de dos genes con impronta opuesta, u2af1-rs1 (impronta materna) y h19 (impronta paterna), en tres estadios del desarrollo de la l\u00ednea germinal masculina de rat\u00f3n: en c\u00e9lulas germinales embrionarias de la l\u00ednea eg-1, derivadas de pgcs de embriones de 8,5 dpc, en espermatogonias de test\u00edculos de 6-7 d\u00edas de edad y en los espermatozoides maduros. En el ensayo se incluy\u00f3, entre otros controles, la l\u00ednea eg-3 derivada de pgcs de ratones de sexo femenino de 8,5 d\u00edas de desarrollo.  el gen u2af1-rs1 se encontr\u00f3 totalmente desmetilado en las c\u00e9lulas germinales embrionarias masculinas y femeninas, lo que indica que el borrador del patr\u00f3n de improtan de este gen se ha producido ya en la l\u00ednea germinal en el embri\u00f3n de 8,5 dpc. El porcentaje de metilaci\u00f3n aument\u00f3 conforme lo hizo el grado de diferenciaci\u00f3n de las c\u00e9lulas analizadas y, sorprendentemente, se correspondi\u00f3 con niveles cada vez mayores de expresi\u00f3n g\u00e9nica.  los espermatozoides no mostraron el patr\u00f3n de metilaci\u00f3n correcto del gen u2af1-rs1, por lo que ser\u00e1n necesarias modificaciones posteriores tras la fecundaci\u00f3n. Por otra parte, observamos la asociaci\u00f3n de una estructura de la cromatina menos compactada con los alelos paternos del gen u2af1-rs1, as\u00ed como tambi\u00e9n la presencia de sitios hipersensibles a la dnasa-i. En las c\u00e9lulas germinales embrionarias femeninas, pero no en las masculinas, se encontr\u00f3 borrado el patr\u00f3n de impronta del gen h19 heredado de los progenitores. el porcentaje de metilaci\u00f3n aument\u00f3 en las c\u00e9lulas germinales m\u00e1s diferenciadas, detect\u00e1ndose la metilaci\u00f3n de ambos alelos en los espermatozoides (patr\u00f3n de impronta establecido). Una estructura de la cromatina menos compactada y la presencia de sitios hipersensibles a la dnasa-i se encontraron asociadas a los alelos maternos no improntados del gen h19.  por otra parte, de los resulta<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>An\u00e1lisis molecular del establecimiento de la impronta en los genes u2af1-rs1 y h19 en la l\u00ednea germinal masculina<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 An\u00e1lisis molecular del establecimiento de la impronta en los genes u2af1-rs1 y h19 en la l\u00ednea germinal masculina <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Zalduendo Macua M. Mar <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Pa\u00eds vasco\/euskal herriko unibertsitatea<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 04\/12\/2002<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Juan  Miguel Ar\u00e9chaga Mart\u00ednez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: ricardo Paniagua g\u00f3mez-\u00e1lvarez <\/li>\n<li> Mazo mart\u00ednez Jes\u00fas del (vocal)<\/li>\n<li>Manuel Jos\u00e9 Gayoso rodriguez (vocal)<\/li>\n<li>Fernando Unda rodr\u00edguez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Zalduendo Macua M. Mar En el presente trabajo se ha analizado el patr\u00f3n de metilaci\u00f3n y la [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[12909],"tags":[61975,13203,9985,61974,14274,61973],"class_list":["post-20355","post","type-post","status-publish","format-standard","hentry","category-pais-vasco-euskal-herriko-unibertsitatea","tag-fernando-unda-rodriguez","tag-juan-miguel-arechaga-Martinez","tag-manuel-jose-gayoso-rodriguez","tag-mazo-Martinez-jesus-del","tag-ricardo-paniagua-gomez-alvarez","tag-zalduendo-macua-m-mar"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/20355","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=20355"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/20355\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=20355"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=20355"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=20355"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}