{"id":20763,"date":"2018-03-09T09:10:13","date_gmt":"2018-03-09T09:10:13","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/actividad-citotoxica-y-antiproliferativa-de-flavonoides-estudio-experimental-in-vitro\/"},"modified":"2018-03-09T09:10:13","modified_gmt":"2018-03-09T09:10:13","slug":"actividad-citotoxica-y-antiproliferativa-de-flavonoides-estudio-experimental-in-vitro","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/murcia\/actividad-citotoxica-y-antiproliferativa-de-flavonoides-estudio-experimental-in-vitro\/","title":{"rendered":"Actividad citot\u00f3xica y antiproliferativa de flavonoides: estudio experimental in vitro"},"content":{"rendered":"<h2>Tesis doctoral de <strong> M. Josefa Y\u00e1\u00f1ez Gascon <\/strong><\/h2>\n<p>Realizamos el estudio de los posibles citot\u00f3xicos (tratamientos durante 24 horas) y antiproliferativos (tratamientos 72 horas) de trece compuestos fen\u00f3licos sobre las l\u00edneas de melanoma b16f10 y sk-mel-1 y de melanocitos no neopl\u00e1sicos melan-a compar\u00e1ndolos con el melfal\u00e1n. Los compuetos fen\u00f3licos no eran solubles en el medio de cultivo siendo necesario disolverlos en dmso, obteniendo una soluci\u00f3n stock a partir de la cual se prepararon las soluciones de trabajo. La m\u00e1xima concentraci\u00f3n ensayada en los compuestos fen\u00f3licos (50 um) estuvo condicionada por la solubilidad de las sustancias y para que la concentraci\u00f3n de dmso en los cultivos no superara el 1%. cuantificamos la viabilidad celular de los cultivos tratados y controles mediante el ensayo colorim\u00e9trico con mmt adaptando los m\u00e9todos de carmichael y alley, que implica el uso de la sal de tetrazolio mtt que es metablizada a una sal coloreada (formaz\u00e1n) por la actividad de la enzima mitocondrial succinato deshidrogenasa. El m\u00e9todo estad\u00edstico empleado consisti\u00f3 en un an\u00e1lisis de la varianza de medidas repetidas complementando con contraste de igualdad de pares con el m\u00e9todo de la m\u00ednima diferencia significativa. los valores de ld50 han sido obtenidos a partir de la curva ajustada a los promedios de los cocientes de absrobancia respecto al control.  nuestros resultados mostraron que ninguno de los compuestos ensayados, sobre las l\u00edneas de melanoma utilizadas, origin\u00f3 un efecto citot\u00f3xico comparable al del melfal\u00e1n que mostr\u00f3 diferencias significativas (p&lt;0,05) respecto a los cultivos tratados con los distintos compuestos fen\u00f3licos. Sobre la l\u00ednea melan-a el efecto fue moderado o nulo excepto el mostrador por la baicaleina y miricetina a concentraciones superiores a 12,5 um. Como observamos a partir de nuestros resultados, las tres l\u00edneas celulares mostraron diferente sensibilidad a los compuestos ensayados: las l\u00edneas de melanoma fueron m\u00e1s sensibles a<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Actividad citot\u00f3xica y antiproliferativa de flavonoides: estudio experimental in vitro<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Actividad citot\u00f3xica y antiproliferativa de flavonoides: estudio experimental in vitro <\/li>\n<li><strong>Autor:<\/strong>\u00a0 M. Josefa Y\u00e1\u00f1ez Gascon <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 17\/12\/2002<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Vicente Vicente Ortega<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal:  Lozano teruel Jos\u00e9 Antonio <\/li>\n<li>Luis Fernando Oton sanchez (vocal)<\/li>\n<li>josep antoni Bomb\u00ed latorre (vocal)<\/li>\n<li>blanca Agulleiro diaz (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de M. Josefa Y\u00e1\u00f1ez Gascon Realizamos el estudio de los posibles citot\u00f3xicos (tratamientos durante 24 horas) y antiproliferativos [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[8235],"tags":[8451,14013,8312,63029,63028,8486],"class_list":["post-20763","post","type-post","status-publish","format-standard","hentry","category-murcia","tag-blanca-agulleiro-diaz","tag-josep-antoni-bombi-latorre","tag-lozano-teruel-jose-antonio","tag-luis-fernando-oton-sanchez","tag-m-josefa-yanez-gascon","tag-vicente-vicente-ortega"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/20763","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=20763"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/20763\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=20763"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=20763"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=20763"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}