{"id":21108,"date":"2003-10-01T00:00:00","date_gmt":"2003-10-01T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/analisis-funcional-de-genes-tarda%c2%ados-de-la-ruta-biosintetica-de-la-pimaricina-de-streptomyces-natalensis-atcc-27448\/"},"modified":"2003-10-01T00:00:00","modified_gmt":"2003-10-01T00:00:00","slug":"analisis-funcional-de-genes-tarda%c2%ados-de-la-ruta-biosintetica-de-la-pimaricina-de-streptomyces-natalensis-atcc-27448","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/leon\/analisis-funcional-de-genes-tarda%c2%ados-de-la-ruta-biosintetica-de-la-pimaricina-de-streptomyces-natalensis-atcc-27448\/","title":{"rendered":"An\u00e1lisis funcional de genes tard\u00edos de la ruta biosint\u00e9tica de la pimaricina de streptomyces natalensis atcc 27448"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Marta Vaz Mendes <\/strong><\/h2>\n<p>La primaricina es una mol\u00e9cula prototipo de los macr\u00f3lidos polienos utilizada en la industria alimentaria y en oftalmolog\u00eda en la prevenci\u00f3n de infecciones f\u00fangicas. La agrupaci\u00f3n g\u00e9nica responsable de la bios\u00edntesis de este antibi\u00f3tico en s.Natalensis atcc 27448 ha sido secuenciada. La secuencia ha desvelado la presencia de cinco genes que codifican para la sintasa de la pimaricina (pims), pimso- pims4, y 13 genes adicionales posiblemente relacionados con la modificaci\u00f3n, regulaci\u00f3n y secreci\u00f3n de la pimaricina.  en esta tesis, se realiza un estudio funcional de los genes de pimd, pimk y pime. El gen pimd codifica para una citocromo p-450 monooxifenasa funcional implicada en la epoxidaci\u00f3n en c4-c5 de la pimaricina. La inactivaci\u00f3n g\u00e9nica de pimd result\u00f3 en la obtenci\u00f3n del mutante s.Natalensis 6d41. La caracterizaci\u00f3n de este mutante mostr\u00f3 que produc\u00eda 4,5- desepoxipimaricina retiene cierta actividad biol\u00f3gica aunque siete veces inferior que la de la pimaricina. La purificaci\u00f3n de la prote\u00edna codificada por pimd, pimd.  el gen pimk codifica para una glucosiltransferasa responsable de la introducci\u00f3n del residuo de micosamina en el anillo macroc\u00edclico de la pimaricinolida. La inactivaci\u00f3n de pimk result\u00f3 en la obtenci\u00f3n del mutante s.Natalensis 1d62 que produce 15-desmicosamina-4,5-desepoxipimaricina. este nuevo compuesto, un precursor de la pimaricina, no present\u00f3 actividad biol\u00f3gica. Al contrario de algunos polienos, el residuo de micosamina resulta ser esencial para la actividad de la pimaricina.  los resultados obtenidos han permitido concluir que los \u00faltimos pasos de la ruta biosint\u00e9tica de la pimaricina, se realizan de una forma secuencial actuando pimd despu\u00e9s de pimk.  el gen pime codifica para una prote\u00edna precursora de una colesterol oxidasa funcional. La inactivaci\u00f3n de este gen origin\u00f3 la cepa no productora de pimaricina, s.Natalensis 1d31.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>An\u00e1lisis funcional de genes tard\u00edos de la ruta biosint\u00e9tica de la pimaricina de streptomyces natalensis atcc 27448<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 An\u00e1lisis funcional de genes tard\u00edos de la ruta biosint\u00e9tica de la pimaricina de streptomyces natalensis atcc 27448 <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Marta Vaz Mendes <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Le\u00f3n<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 10\/01\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Jes\u00fas Fern\u00e1ndez Aparicio<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jos\u00e9 Antonio Salas fernandez <\/li>\n<li>ram\u00f3n Santamar\u00eda s\u00e1nchez (vocal)<\/li>\n<li>Jes\u00fas Navas m\u00e9ndez (vocal)<\/li>\n<li> Calisto laureano santos alves vieira Mar\u00eda  da gra\u00ed\u00a7a (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Marta Vaz Mendes La primaricina es una mol\u00e9cula prototipo de los macr\u00f3lidos polienos utilizada en la industria [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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