{"id":21446,"date":"2018-03-09T09:11:14","date_gmt":"2018-03-09T09:11:14","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-de-motivos-estructurales-implicados-en-la-estabilizacion-y-la-actividad-de-la-histidina-descarboxilasa-de-rata\/"},"modified":"2018-03-09T09:11:14","modified_gmt":"2018-03-09T09:11:14","slug":"caracterizacion-de-motivos-estructurales-implicados-en-la-estabilizacion-y-la-actividad-de-la-histidina-descarboxilasa-de-rata","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/malaga\/caracterizacion-de-motivos-estructurales-implicados-en-la-estabilizacion-y-la-actividad-de-la-histidina-descarboxilasa-de-rata\/","title":{"rendered":"Caracterizaci\u00f3n de motivos estructurales implicados en la estabilizaci\u00f3n y la actividad de la histidina descarboxilasa de rata"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Daniel Rodr\u00edguez Agudo <\/strong><\/h2>\n<p>La tesis doctoral define 5 objetivos fundamentales:  1,- el estudio del posible papel del extremo carboxilo terminal de la histidina descarboxilasa (hdc) de mam\u00edferos en la degradaci\u00f3n dependiente de atp.  2,- el estudio de la posible degradaci\u00f3n de la hdc por sistemas proteol\u00edticos dependientes de calcio (calpa\u00ednas).  3,- el establecimiento de un protocolo de purificaci\u00f3n de la enzima recombinante (residuos 1\/512) en cantidades semi-preparativas.  4,- el estudio de los efectos inhibidores de algunos an\u00e1logos de substrato, y finalmente.  5,- el estudio de la implicaci\u00f3n de algunos residuos en la cat\u00e1lisis o estructura de la prote\u00edna mediante la estrategia de mutag\u00e9nesis dirigida.  los dos primeros objetivos se llevan a cabo a partir de distintas versiones recombinantes expresadas in vitro; cuya degradaci\u00f3n se realiza, igualmente in vitro en sistemas enriquecidos en distintas proteasas o reconstituidos a partir de sus componentes.  los otros tres objetivos tienen como material de trabajo preparaciones altamente purificadas de la enzima salvaje, o mutantes generados a partir de ella, que contienen 512 residuos del extremo n-terminal de la prote\u00edna deducida de su cdna.  la caracterizaci\u00f3n de la versi\u00f3n salvaje y los efectos de inhibidores y mutaciones se estudian mediante espectrofotometr\u00eda uv-vis, fluorescencia, electroforesis convencionales sds\/page y no reductoras, transferencias western, y medidas de actividades enzim\u00e1ticas en funci\u00f3n de la capacidad de descarboxilaci\u00f3n de histidina marcada radiactivamente. La discusi\u00f3n se apoya sobre un modelo 3d de la prote\u00edna generada mediante programas bioinform\u00e1ticos de predicci\u00f3n de estructura de prote\u00ednas.  las conclusiones de este trabajo se resumen en los siguientes puntos:  1,- el extremo carboxilo de la hdc no puede reemplazar al extremo carboxilo de la ornitina descarboxilasa en su papel en la degradaci\u00f3n de esta prote\u00edna por el proteasoma 26s, por tanto,<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizaci\u00f3n de motivos estructurales implicados en la estabilizaci\u00f3n y la actividad de la histidina descarboxilasa de rata<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizaci\u00f3n de motivos estructurales implicados en la estabilizaci\u00f3n y la actividad de la histidina descarboxilasa de rata <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Daniel Rodr\u00edguez Agudo <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 M\u00e1laga<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 31\/01\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Francisca Mar\u00eda Sanchez Jimenez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Juan Carbonell gisbert <\/li>\n<li>ignacio Jos\u00e9 Fajardo paredes (vocal)<\/li>\n<li>claudia Ruiz-capillas p\u00e9rez (vocal)<\/li>\n<li>eugenio Gimenez gutierrez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Daniel Rodr\u00edguez Agudo La tesis doctoral define 5 objetivos fundamentales: 1,- el estudio del posible papel del 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