{"id":21750,"date":"2018-03-09T09:11:39","date_gmt":"2018-03-09T09:11:39","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/clonaje-y-caracterizacion-de-una-ent-copalill-difosfato-sintasa-de-citrange-carrizo-y-efecto-de-su-sobreexpresion-en-plantas-de-tabaco\/"},"modified":"2018-03-09T09:11:39","modified_gmt":"2018-03-09T09:11:39","slug":"clonaje-y-caracterizacion-de-una-ent-copalill-difosfato-sintasa-de-citrange-carrizo-y-efecto-de-su-sobreexpresion-en-plantas-de-tabaco","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/politecnica-de-valencia\/clonaje-y-caracterizacion-de-una-ent-copalill-difosfato-sintasa-de-citrange-carrizo-y-efecto-de-su-sobreexpresion-en-plantas-de-tabaco\/","title":{"rendered":"Clonaje y caracterizaci\u00f3n de una ent-copalill difosfato sintasa de citrange carrizo y efecto de su sobreexpresi\u00f3n en plantas de tabaco"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Carmen Pilar Alapont Asensio <\/strong><\/h2>\n<p>Las giberelinas (gas) son hormonas que intervienen en numerosos aspectos del desarrollo vegetal, incluyendo la elongaci\u00f3n del tallo.  el primer caso en la ruta de bios\u00edntesis de gas es la conversi\u00f3n del geranilgeranil difosfato (ggpp) a ent-kaureno, v\u00eda copalil difosfato (cpp). estas dos reacciones est\u00e1n catalizadas por las ciclasas ent-copalil difosfato sintasa (cps) y ent-kaureno sintasa (ks), respectivamente. En este trabajo se ha aislado un clon de cdna, ccps1, que codifica una cps del h\u00edbrido citrange carrizo (citrus sintesis (l.) Obs x poncirus trifoliata (l.) Raf, ampliamente utilizado como patr\u00f3n en c\u00edtricos) y se ha evaluado el efecto de su sobreexpresi\u00f3n sobre el fenotipo de plantas transg\u00e9nicas de tabaco. tambi\u00e9n se ha obtenido un clon parcial de un gen, ccps2, que posiblemente codifica una segunda cps en citrange carrizo. Se ha desmostrado que cccps1 procede del parental poncirus trifoliata, mientras que cccps2 procede de citrus sinensis, el otro parental de citrange carrizo. Se ha obtenido la secuencia de dna gen\u00f3mico correspondiente a la regi\u00f3n codificante del gen cccps1 y se ha encontrado que este gen codifica tras prote\u00ednas diferentes, denominadas cccps1a, cccps1b, cccpsc, como consecuencia de un mecanismo de corte-empalme alternativo en el proceso de maduraci\u00f3n del pre-rnam. sin embargo, \u00fanicamente los productos de expresi\u00f3n en e.Coli del clon cccps1b son capaces de metabolizar ggpp a cpp in vitro. Cccps1 se expresa en muy bajos niveles y su expresi\u00f3n est\u00e1 limitada a \u00f3rganos vegetativos y reproductivos en crecimiento activo. La expresi\u00f3n de cccps1 se induce a corto plazo por un aumento en la temperatura, y tambi\u00e9n mediante la aplicaci\u00f3n de inhibidores de la ruta de bios\u00edntesis de gas. Plantas transg\u00e9nicas homocigotas de nicotiana tabacum que sobreexpresan el clon cccps1b de citrange carrizo eran m\u00e1s altas (entre9-12%) y ten\u00edan un fenotipo de inflorescencias y entrenudos y ped\u00fanculos florales m\u00e1<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Clonaje y caracterizaci\u00f3n de una ent-copalill difosfato sintasa de citrange carrizo y efecto de su sobreexpresi\u00f3n en plantas de tabaco<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Clonaje y caracterizaci\u00f3n de una ent-copalill difosfato sintasa de citrange carrizo y efecto de su sobreexpresi\u00f3n en plantas de tabaco <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Carmen Pilar Alapont Asensio <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Polit\u00e9cnica de Valencia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 21\/02\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Jos\u00e9 Luis Garc\u00eda Mart\u00ednez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: vicente Moreno ferrero <\/li>\n<li>isabel Arrillaga mateos (vocal)<\/li>\n<li>Mar\u00eda  dolores Rodriguez martin (vocal)<\/li>\n<li>pedro Carrasco sorl\u00ed (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Carmen Pilar Alapont Asensio Las giberelinas (gas) son hormonas que intervienen en numerosos aspectos del desarrollo vegetal, [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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