{"id":21810,"date":"2018-03-09T09:11:44","date_gmt":"2018-03-09T09:11:44","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/estudio-de-la-secrecion-de-protea%c2%adnas-por-el-sistema-autotransportador-de-bacterias-gram-negativas\/"},"modified":"2018-03-09T09:11:44","modified_gmt":"2018-03-09T09:11:44","slug":"estudio-de-la-secrecion-de-protea%c2%adnas-por-el-sistema-autotransportador-de-bacterias-gram-negativas","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/estudio-de-la-secrecion-de-protea%c2%adnas-por-el-sistema-autotransportador-de-bacterias-gram-negativas\/","title":{"rendered":"Estudio de la secreci\u00f3n de prote\u00ednas por el sistema autotransportador de bacterias gram-negativas"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Esteban Veiga Chacon <\/strong><\/h2>\n<p>En esta tesis se presentan una serie de datos que se resumen en las siguientes conclusiones:  1,- el sistema de secreci\u00f3n de las prote\u00ednas autotransportadoras (ats), cuyo  arquetipo es la proteasa iga de neisseria, permite la presentaci\u00f3n ect\u00f3pica de polip\u00e9ptidos de muy diversas caracter\u00edsticas sobre la superficie celular de e.Coli a base de generar fusiones entre el dominio c-terminal del autotransportador (c-igap) con la secuencia polipept\u00eddica de inter\u00e9s.  2,- la capacidad de presentaci\u00f3n superficial de polip\u00e9ptidos heter\u00f3logos por los sistemas autotransportadores incluyen no s\u00f3lo secuencias relativamente cortas sin puentes disulfuro, sino tambi\u00e9n prote\u00ednas globulares con enlaces s-s.  3,- la presentaci\u00f3n superficial de secuencias de dimerizaci\u00f3n jun y fos procedentes de activadores transcripcionales eucari\u00f3ticos mediante fusiones a c-igap confiri\u00f3 a las correspondentes c\u00e9lulas nuevas propiedades de ahesi\u00f3n mutua de forma dependiente de la expresi\u00f3n controlada de las fusiones jun-c-igap o fos-c-igap.  4,- las fusiones entre el dominio c-iga de los ats y m\u00f3dulos scfvs alcanzaron un grado de presentaci\u00f3n superficial del anticuerpo correctamente estructurado en torno al 20% de la prote\u00edna h\u00edbrida total expresada. Tal estructuraci\u00f3n, en particular la formaci\u00f3n de puentes disulfuro, dependi\u00f3 enteramente de la acci\u00f3n de chaperonas peripl\u00e1smicas. Esta dependencia se mantuvo en el caso de la presentaci\u00f3n de anticuerpos de camello, si bien en este caso la presentaci\u00f3n y actividad fue del 100%.  5,- los autotransportadores tienen la capacidad de exponer distintas actividades biol\u00f3gicas sobre la superficie de bacterias gram-negativas. Hemos demostrado el uso de estos at para exponer hacia el medio extracelular distintas prote\u00ednas que redirigen de forma espec\u00edfica la adhesi\u00f3n bacteriana. Tambi\u00e9n, experimentos in vivo indican que podemos usar bacterias que expresan un scfv, con actividad neutralizante del v<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Estudio de la secreci\u00f3n de prote\u00ednas por el sistema autotransportador de bacterias gram-negativas<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Estudio de la secreci\u00f3n de prote\u00ednas por el sistema autotransportador de bacterias gram-negativas <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Esteban Veiga Chacon <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 26\/02\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Victor De Lorenzo Prieto<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: fernando De la cruz calahorra <\/li>\n<li>josep Casadesus pursals (vocal)<\/li>\n<li>p. Pugsley anthony (vocal)<\/li>\n<li>miquel Coll capella (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Esteban Veiga Chacon En esta tesis se presentan una serie de datos que se resumen en las [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[1],"tags":[65567,10595,9514,26322,65568,4203],"class_list":["post-21810","post","type-post","status-publish","format-standard","hentry","category-sin-categoria","tag-esteban-veiga-chacon","tag-fernando-de-la-cruz-calahorra","tag-josep-casadesus-pursals","tag-miquel-coll-capella","tag-p-pugsley-anthony","tag-victor-de-lorenzo-prieto"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/21810","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=21810"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/21810\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=21810"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=21810"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=21810"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}