{"id":22022,"date":"2003-10-03T00:00:00","date_gmt":"2003-10-03T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/purificacion-y-caracterizacion-bioqua%c2%admica-de-poligalacturonasas-inducidas-en-aspergillus-niger-con-pectina-de-ca%c2%adtrico\/"},"modified":"2003-10-03T00:00:00","modified_gmt":"2003-10-03T00:00:00","slug":"purificacion-y-caracterizacion-bioqua%c2%admica-de-poligalacturonasas-inducidas-en-aspergillus-niger-con-pectina-de-ca%c2%adtrico","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/burgos\/purificacion-y-caracterizacion-bioqua%c2%admica-de-poligalacturonasas-inducidas-en-aspergillus-niger-con-pectina-de-ca%c2%adtrico\/","title":{"rendered":"Purificaci\u00f3n y caracterizaci\u00f3n bioqu\u00edmica de poligalacturonasas inducidas en aspergillus niger con pectina de c\u00edtrico"},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Brizuela Fern\u00e1ndez M. Paloma <\/strong><\/h2>\n<p>El objetivo principal de este trabajo de investigaci\u00f3n se centra en el dise\u00f1o de un protocolo de purificaci\u00f3n de las poligalacturonasas de aspergillus niger cect 2088, mediante el cual se obtengan niveles adecuados de rendimiento y un alto grado de purificaci\u00f3n.  aspergillus niger sintetiza m\u00faltiples formas de poligalacturonasa, cuya expresi\u00f3n puede regularse por factores tales como el tipo de cepa utilizada, las condiciones de crecimiento microbiano y el inductor utilizado.  por este motivo, inicialmente se estudi\u00f3 la influencia del medio de proliferaci\u00f3n del hongo en la producci\u00f3n de isoenzimas, estableci\u00e9ndose como condiciones \u00f3ptimas para la producci\u00f3n de actividad poligalacturonasa, el crecimiento del microorganismo en el medio czapek-dox y la posterior inducci\u00f3n con pectina de c\u00edtrico.  teniendo en cuenta que el extracto crudo donde se encuentran las enzimas a purificar es un medio f\u00fangico extracelular, se plante\u00f3 un proceso de purificaci\u00f3n en el que se comenz\u00f3 aplicando t\u00e9cnicas poco resolutivas que permitiesen concentrar la prote\u00edna en un m\u00ednimo volumen de trabajo, como la ultrafiltraci\u00f3n y la precipitaci\u00f3n fraccional con sulfato am\u00f3nico. Posteriormente, se aplicaron t\u00e9cnicas de mayor resoluci\u00f3n como la cromatograf\u00eda de permeabilidad en gel y la cromatograf\u00eda de intercambio i\u00f3nico. El proceso inclu\u00eda por tanto cuatro etapas de bajo coste econ\u00f3mico y aplicables a gran escala.  como resultado del proceso global de purificaci\u00f3n se obtuvieron cuatro fracciones con actividad poligalacturonasa, una de ellas, completamente purificada. Su posterior caracterizaci\u00f3n permiti\u00f3 obtener un mayor conocimiento de las isoenzimas y rentabilizar sus aplicaciones industriales. As\u00ed, los pesos moleculares de las cuatro poligalacturonasa aisladas fueron 73.811, 40.620, 59.030 y 59.470 da. Adem\u00e1s, todas ellas presentaban puntos isoel\u00e9ctricos \u00e1cidos iguales o inferiores a 4,00 y mostraban diferente afinidad por el<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Purificaci\u00f3n y caracterizaci\u00f3n bioqu\u00edmica de poligalacturonasas inducidas en aspergillus niger con pectina de c\u00edtrico<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Purificaci\u00f3n y caracterizaci\u00f3n bioqu\u00edmica de poligalacturonasas inducidas en aspergillus niger con pectina de c\u00edtrico <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Brizuela Fern\u00e1ndez M. Paloma <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Burgos<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 10\/03\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Pilar Mu\u00f1iz Rodriguez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: manuel P\u00e9rez mateos <\/li>\n<li>Mar\u00eda  teresa Agapito serrano (vocal)<\/li>\n<li>guillermo Saez tormo (vocal)<\/li>\n<li>eulalia Alonso iglesias (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Brizuela Fern\u00e1ndez M. Paloma El objetivo principal de este trabajo de investigaci\u00f3n se centra en el dise\u00f1o [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[37674],"tags":[66095,37233,12108,50710,57260,27086],"class_list":["post-22022","post","type-post","status-publish","format-standard","hentry","category-burgos","tag-brizuela-fernandez-m-paloma","tag-eulalia-alonso-iglesias","tag-guillermo-saez-tormo","tag-manuel-perez-mateos","tag-maria-teresa-agapito-serrano","tag-pilar-muniz-rodriguez"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/22022","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=22022"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/22022\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=22022"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=22022"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=22022"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}