{"id":22207,"date":"2018-03-09T09:12:17","date_gmt":"2018-03-09T09:12:17","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-expresion-y-regulacion-del-locus-en-el-que-se-encuentra-el-gen-que-codifica-para-la-metionina-adenosiltransferasa-gen-mat2-en-el-tripanosomatido-leishmania-infantum\/"},"modified":"2018-03-09T09:12:17","modified_gmt":"2018-03-09T09:12:17","slug":"caracterizacion-expresion-y-regulacion-del-locus-en-el-que-se-encuentra-el-gen-que-codifica-para-la-metionina-adenosiltransferasa-gen-mat2-en-el-tripanosomatido-leishmania-infantum","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/leon\/caracterizacion-expresion-y-regulacion-del-locus-en-el-que-se-encuentra-el-gen-que-codifica-para-la-metionina-adenosiltransferasa-gen-mat2-en-el-tripanosomatido-leishmania-infantum\/","title":{"rendered":"Caracterizaci\u00f3n, expresi\u00f3n y regulaci\u00f3n del locus en el que se encuentra el gen que codifica para la metionina adenosiltransferasa (gen mat2) en el tripanosom\u00e1tido leishmania infantum"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Carlos Garc\u00eda Estrada <\/strong><\/h2>\n<p>En este trabajo se ha caracterizado el locus en el que se encuentra el gen mat2 que codifica para la metionina adenosiltransferasa (mat) en el protozoo par\u00e1sito leishmania infantum. Al contrario que la mat de mam\u00edferos, en este microorganismo esta prote\u00edna est\u00e1 codificada por dos copias g\u00e9nicas id\u00e9nticas de 1179 pb dispuestas en t\u00e1ndem cabeza-cola y separadas por una regi\u00f3n espaciadora de 3953 pb. El an\u00e1lisis de la secuencia de la regi\u00f3n del genoma que contiene el gen mat2, revel\u00f3 la existencia de un orf de 1113 pb precediendo a cada una de las copias de dicho gen. Mediante an\u00e1lisis de northen se comprob\u00f3 que el orf se trataba de un nuevo gen, a cuya secuencia te\u00f3rica de amino\u00e1cidos se le denomin\u00f3 de northern se comprob\u00f3 que el orf se trataba de un nuevo gen, a cuya secuencia te\u00f3rica de amino\u00e1cidos se le denomin\u00f3 lorien (leishmania open readingframe intergenic expresed newprotein).  la agrupaci\u00f3n de genes lorieni mat2 se localiz\u00f3 en un cromosoma de 1,5 mb mdiante la realizaci\u00f3n de electroforesis en campo pulsado. El origen de transcripci\u00f3n de dicho \u00abcluster\u00bb se identific\u00f3 a trav\u00e9s del empleo de ensayos de \u00abrun-on\u00bb nuclear, en la regi\u00f3n situada entre las posiciones -2135\/-1446 respecto al cod\u00f3n de inicio de la primera copia del gen lorien.  los genes mat2 y lorien se expresan de un modo constituido en promastigotes de l.Inantum, existiendo dos transcritos para el primero de ellos (2,3 y 2,6 kb) y un \u00fanico arnm para el segundo (2,2 kb). A trav\u00e9s de experimentos de western se comprob\u00f3 que el arnm de los genes mat2 y lorien se traduc\u00edan a una prote\u00edna de 49 y 47 kda, respectivamente. Estos tama\u00f1os resultaron ligeramente superiores a los estimados te\u00f3ricamente a partir de sus respectivas secuencias.  a pesar de la poca homolog\u00eda que mostr\u00f3 la prote\u00edna lorien con el resto de las prote\u00ednas de las bases de datos, se vio que conten\u00eda un dominio conservado consistente en un dedo de zinc de tipo sp-ring\/miz c<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizaci\u00f3n, expresi\u00f3n y regulaci\u00f3n del locus en el que se encuentra el gen que codifica para la metionina adenosiltransferasa (gen mat2) en el tripanosom\u00e1tido leishmania infantum<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizaci\u00f3n, expresi\u00f3n y regulaci\u00f3n del locus en el que se encuentra el gen que codifica para la metionina adenosiltransferasa (gen mat2) en el tripanosom\u00e1tido leishmania infantum <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Carlos Garc\u00eda Estrada <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Le\u00f3n<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 24\/03\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Rosa Mar\u00eda Reguera Torres<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: paloma (secretario) Liras pad\u00edn <\/li>\n<li>Jos\u00e9 Mar\u00eda Requena rolan\u00eda (vocal)<\/li>\n<li> Pajares taranc\u00f3n Mar\u00eda de los \u00e1ngeles (vocal)<\/li>\n<li>  (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Carlos Garc\u00eda Estrada En este trabajo se ha caracterizado el locus en el que se encuentra el [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[4430],"tags":[66533,66536,66537,66535,66534],"class_list":["post-22207","post","type-post","status-publish","format-standard","hentry","category-leon","tag-carlos-garcia-estrada","tag-jose-maria-requena-rolania","tag-pajares-tarancon-maria-de-los-angeles","tag-paloma-secretario-liras-padin","tag-rosa-maria-reguera-torres"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/22207","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=22207"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/22207\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=22207"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=22207"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=22207"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}