{"id":22347,"date":"2018-03-09T09:12:29","date_gmt":"2018-03-09T09:12:29","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/identificacion-y-caracterizacion-de-una-nueva-adn-polimerasa-eucariotica-la-adn-polimerasa-lambda\/"},"modified":"2018-03-09T09:12:29","modified_gmt":"2018-03-09T09:12:29","slug":"identificacion-y-caracterizacion-de-una-nueva-adn-polimerasa-eucariotica-la-adn-polimerasa-lambda","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/identificacion-y-caracterizacion-de-una-nueva-adn-polimerasa-eucariotica-la-adn-polimerasa-lambda\/","title":{"rendered":"Identificaci\u00f3n y caracterizaci\u00f3n de una nueva adn polimerasa eucari\u00f3tica: la adn polimerasa lambda"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Miguel Garc\u00eda D\u00edaz <\/strong><\/h2>\n<p>Mediante b\u00fasquedas por analog\u00eda de secuencia se ha identificado la secuencia de un nuevo gen humano que codifica una nueva adn polimerasa de la familia x. Esta nueva prote\u00edna se ha denominado adn polimerasa lambda (pol). La pol es hom\u00f3loga a la pol beta, una adn polimerasa de reparaci\u00f3n que participa en mam\u00edferos en el proceso de reparaci\u00f3n por escisi\u00f3n de base. La pol se expresa en todos los tejidos analizados, con especial preponderancia en test\u00edculo. Adem\u00e1s, su expresi\u00f3n es exclusivamente nuclear.  se ha llevado a cabo la sobreexpresi\u00f3n de la pol en c\u00e9lulas de e.Coli, as\u00ed como su purificaci\u00f3n, demostrando que la pol posee una actividad adn polimerasa intr\u00ednseca.  la caracterizaci\u00f3n de sus caracter\u00edsticas enzim\u00e1ticas ha permitido comprobar que se trata de un enzima dirigido por el molde y distributivo, aunque su procesividad se incrementa en sustrato de tipo gap, lo que sugiere que estos moldes podr\u00edan ser su sustrato fisiol\u00f3gico. La medida de los par\u00e1metros cin\u00e9ticos de la pol ha permitido demostrar que se trata de un enzima con una elevada afinidad por nucloe\u00f3tico, sugiriendo un papel de este enzima en situaciones en que los niveles de precursores nucleot\u00eddicos se encuentren disminuidos.  adem\u00e1s de su actividad de polimerizaci\u00f3n, la pol posee un actividad desoxirribosa fosfato liasa (drp liasa), lo que sugiere su participaci\u00f3n en el proceso de reparaci\u00f3n por escisi\u00f3n de base. De hecho, es posible reconstruir la reacci\u00f3n de reparaci\u00f3n de un uracilo in vitro empleando pol.  se han llevado a cabo medidas de la fidelidad de s\u00edntesis de la pol humana. en cuanto a las sustituciones de base, se trata de un enzima con una fidelidad modesta, algo inferior a la de la pol beta. Sin embargo, la pol comete con una frecuencia extraordinaria errores de deleci\u00f3n: se trata de la adn polimerasa caracterizada hasta el momento con la mayor frecuencia de comisi\u00f3n de errores de deleci\u00f3n.  el an\u00e1lisis de los errores<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Identificaci\u00f3n y caracterizaci\u00f3n de una nueva adn polimerasa eucari\u00f3tica: la adn polimerasa lambda<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Identificaci\u00f3n y caracterizaci\u00f3n de una nueva adn polimerasa eucari\u00f3tica: la adn polimerasa lambda <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Miguel Garc\u00eda D\u00edaz <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 31\/03\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Luis Blanco D\u00e1vila<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: margarita Salas falgueras <\/li>\n<li>Andr\u00e9s Aguilera l\u00f3pez (vocal)<\/li>\n<li>Mar\u00eda antonia Blasco marhuenda (vocal)<\/li>\n<li>Jos\u00e9 Fernandez piqueras (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Miguel Garc\u00eda D\u00edaz Mediante b\u00fasquedas por analog\u00eda de secuencia se ha identificado la secuencia de un nuevo [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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