{"id":22465,"date":"2003-07-04T00:00:00","date_gmt":"2003-07-04T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/clonado-expresion-caracterizacion-y-modelizacion-de-la-adn-topoisomerasa-i-en-el-tripanosomatido-leishmania-infantum\/"},"modified":"2003-07-04T00:00:00","modified_gmt":"2003-07-04T00:00:00","slug":"clonado-expresion-caracterizacion-y-modelizacion-de-la-adn-topoisomerasa-i-en-el-tripanosomatido-leishmania-infantum","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/leon\/clonado-expresion-caracterizacion-y-modelizacion-de-la-adn-topoisomerasa-i-en-el-tripanosomatido-leishmania-infantum\/","title":{"rendered":"Clonado, expresi\u00f3n, caracterizaci\u00f3n y modelizaci\u00f3n de la adn topoisomerasa i en el tripanosom\u00e1tido leishmania infantum"},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Otero Marcos Ana Rosa <\/strong><\/h2>\n<p>La adn topoisomerasa i cataliza la relajaci\u00f3n de adn superenrollado mediante un mecanismo de corte transitotio en una hebra y la formaci\u00f3n de un enlace fosfodi\u00e9ster entre el extremo del adn y la tirosina del centro activo. Una caracter\u00edstica com\u00fan a las adn topoisomerasas i eucariotas es la presencia de un centro activo tsk-ny con la tirosina como amino\u00e1cido catal\u00edtico.  la camptotecina (cpt) es un veneno espec\u00edfico de adn topoisomerasas i que estabiliza de manera reversible el intermediario covalente enzima-adn inhibiendo el religado del adn. Aunque la estabilizaci\u00f3n del intermediario covalente no es citot\u00f3xica per se, la letalidad de la cpt se explica durante la fase s del ciclo celular, por su interferencia con el avance de la horquilla de replicaci\u00f3n, lo que provoca la conversi\u00f3n del corte de una \u00fanica cadena del adn en un corte de la doble cadena del adn potencialmente letal. Todo ello produce la interrupci\u00f3n del ciclo celular en fase g2 y posteriormente muerte celular.  los promastigotes de leishmania infantum mostraron sensibilidad frente a cpt, con una ec50 menor de 0,5 um, prueba de la existencia de una adn topoisomerasa i en este par\u00e1sito.  en primer lugar se clon\u00f3 el gen codificante de la subunidad a (top1a) que present\u00f3 gran homolog\u00eda con distintas adn topoisomerasas eucariotas pero que carec\u00eda del grupo conservado tsk-ny, dando lugar a distintas teor\u00edas sobre la reconstruci\u00f3n de una topoisomerasa activa en este par\u00e1sito para posteriormente clonar u nuevo gen, top 1b, que contiene el grupo tskiny. as\u00ed pues, l.Infantum posee una adn topoisomerasa i heterodim\u00e9trica, constitu\u00edda por dos subunidades estructurales diferentes.  la subunidad a porta los sitios de uni\u00f3n al adn (dominio \u00abcore\u00bb) y la subunidad b el centro catal\u00edtico (dominio c-terminal). En un sistema de expresi\u00f3n en levaduras, los lisados procedentes de la sobreexpresi\u00f3n de los genes top1a y top1b por separado no condujeron a la producc<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Clonado, expresi\u00f3n, caracterizaci\u00f3n y modelizaci\u00f3n de la adn topoisomerasa i en el tripanosom\u00e1tido leishmania infantum<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Clonado, expresi\u00f3n, caracterizaci\u00f3n y modelizaci\u00f3n de la adn topoisomerasa i en el tripanosom\u00e1tido leishmania infantum <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Otero Marcos Ana Rosa <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Le\u00f3n<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 07\/04\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Rosa Mar\u00eda Reguera Torres<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: david Ordo\u00f1ez escudero <\/li>\n<li> G\u00f3mez fern\u00e1ndez Juan  carmelo (vocal)<\/li>\n<li>Antonio  Juan Garcia fernandez (vocal)<\/li>\n<li>montserrat Port\u00fas vinyeta (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Otero Marcos Ana Rosa La adn topoisomerasa i cataliza la relajaci\u00f3n de adn superenrollado mediante un mecanismo [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[4430],"tags":[24996,4565,8563,33726,67143,66534],"class_list":["post-22465","post","type-post","status-publish","format-standard","hentry","category-leon","tag-antonio-juan-garcia-fernandez","tag-david-ordonez-escudero","tag-gomez-fernandez-juan-carmelo","tag-montserrat-portus-vinyeta","tag-otero-marcos-ana-rosa","tag-rosa-maria-reguera-torres"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/22465","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=22465"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/22465\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=22465"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=22465"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=22465"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}