{"id":22575,"date":"2003-11-04T00:00:00","date_gmt":"2003-11-04T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/valor-de-la-linea-celular-murina-fbmd-1-para-el-estudio-de-las-celulas-hematopoyeticas-humanas\/"},"modified":"2003-11-04T00:00:00","modified_gmt":"2003-11-04T00:00:00","slug":"valor-de-la-linea-celular-murina-fbmd-1-para-el-estudio-de-las-celulas-hematopoyeticas-humanas","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/salamanca\/valor-de-la-linea-celular-murina-fbmd-1-para-el-estudio-de-las-celulas-hematopoyeticas-humanas\/","title":{"rendered":"Valor de la linea celular murina fbmd-1 para el estudio de las c\u00e9lulas hematopoy\u00e9ticas humanas"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Carmen Pata Iglesias <\/strong><\/h2>\n<p>El estroma humano procedente de poblaciones celulares de m\u00e9dulas \u00f3seas (mo) de donantes sanos, es el sustrato utilizado habitualmente como microambiente est\u00e1ndar para estudiar \u00abin vitro\u00bb la funcionalidad de los progenitores hematopoy\u00e9ticos humanos. Una alternativa a la utilizaci\u00f3n de mo normales es la utilizaci\u00f3n de l\u00edneas celulares de estroma que sean capaces de soportar la hematopoyesis humana.  en el presente trabajo intentamos comprobar si el estroma murino fmbd-1 tiene la misma capacidad del estroma normal humano para sustentar la hematopoyesis humana tanto normal como patol\u00f3gica.  se realizaron clp en dos etapas y en paralelo estableciendo la capa de estroma con c\u00e9lulas de mo de donantes sanos y con la l\u00ednea murina fmbd-1. se realizaron segundos in\u00f3culos con: 55 productos de af\u00e9resis, 17 mo normales, 39 mo de pacientes con smd y 12 mo de pacientes con lmc ph(+) en el momento del diagn\u00f3stico. Los cultivo se valoraron por la producci\u00f3n de progenitores granulomonoc\u00edticos (cfu-gm) y c\u00e9lulas mononucleadas (cmn) al sobrenadante.  el \u00e1nalisis de los clp realizados mostr\u00f3 que la cantidad total y semanal de cmn y cfu-gm fue significativamente superior en los cultivos establecidos sobre estroma normal humano. Los progenitores inmaduros estudiados mediante clp sobre estroma fbmd-1 se correlacionaron significativamente con los progenitores analizados mediante otras metodolog\u00edas; ensayos delta (r=0,976; p= 0,001) y c\u00e9lulas cd34+ analizadas por citometr\u00eda de flujo (r=0,690; p=0,010). No encotramos correlaci\u00f3n entre los resultados obtenidos cuando se utiliza humano.  en los pacientes con lmc, las c\u00e9lulas ph(+), analizadas por fish, disminuyeron progresivamente tras el clp con un comportamiento similar con ambas capas.  en conclusi\u00f3n, el estroma humano promueve de forma m\u00e1s eficaz la proliferaci\u00f3n de las c\u00e9lulas progenitoras hematopoy\u00e9ticas. Sin embargo, el estroma fbmd-1 parece ser m\u00e1s \u00fatil para estandarizar la me<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Valor de la linea celular murina fbmd-1 para el estudio de las c\u00e9lulas hematopoy\u00e9ticas humanas<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Valor de la linea celular murina fbmd-1 para el estudio de las c\u00e9lulas hematopoy\u00e9ticas humanas <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Carmen Pata Iglesias <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Salamanca<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 11\/04\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Mar\u00eda  Consuelo Juan a Del Ca\u00f1izo Fernandez Roldan<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: agustin Rios gonzalez <\/li>\n<li> Garc\u00eda g\u00f3mez m. Luisa (vocal)<\/li>\n<li> Fern\u00e1ndez calvo Francisco Javier (vocal)<\/li>\n<li>  (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Carmen Pata Iglesias El estroma humano procedente de poblaciones celulares de m\u00e9dulas \u00f3seas (mo) de donantes sanos, [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[9386],"tags":[9428,67395,55142,67396,9401],"class_list":["post-22575","post","type-post","status-publish","format-standard","hentry","category-salamanca","tag-agustin-rios-gonzalez","tag-carmen-pata-iglesias","tag-fernandez-calvo-francisco-javier","tag-garcia-gomez-m-luisa","tag-maria-consuelo-juan-a-del-canizo-fernandez-roldan"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/22575","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=22575"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/22575\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=22575"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=22575"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=22575"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}