{"id":22858,"date":"2003-09-05T00:00:00","date_gmt":"2003-09-05T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/recombinacion-del-v-i-h-in-vitro\/"},"modified":"2003-09-05T00:00:00","modified_gmt":"2003-09-05T00:00:00","slug":"recombinacion-del-v-i-h-in-vitro","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/recombinacion-del-v-i-h-in-vitro\/","title":{"rendered":"Recombinaci\u00f3n del v.i.h. in vitro"},"content":{"rendered":"<h2>Tesis doctoral de <strong> M. Jos\u00e9 Iglesias Sanchez <\/strong><\/h2>\n<p>Este trabajo se ha centrado en el estudio de la influencia que ejerce la recombinaci\u00f3n en la evoluci\u00f3n de virus de la inmunodeficiencia humana (vih), ya que se trata del principal mecanismo de variaciones este virus.  en primer lugar se realizaron una serie de infecciones dobles con diferencias combinaciones de dos cepas del vih-1, en c\u00e9lulas mt4 (l\u00ednea linfoc\u00edtica humana), que se mantuvieron en cultivo durante 22 d\u00edas. Los adns procedentes de varios d\u00edas a lo largo de los cultivos se analizaron mediante vrios t\u00e9cnicas puestas a punto previamente. Los resultados pusieron de manifiesto la influencia de varios factores como el tiempo, la multiplicidad de infecci\u00f3n y la distancia gen\u00e9tica sobre la proporci\u00f3n de recombinantes en las progenies virales obtenidas.  la utilizaci\u00f3n de una pcr cuantitativa espec\u00edfica para la determinaci\u00f3n de la proporci\u00f3n de recombinantes en los adns resultantes de las infecciones dobles, permiti\u00f3 determinar una relaci\u00f3n inversamente proporcional entre la distancia gen\u00e9tica entre los virus parentales y la proporci\u00f3n de virus recombinantes que origienan. Al mismo tiempo se observ\u00f3 un aumento progresivo de esta proporci\u00f3n a lo largo del tiempo, en dos de estas infecciones.  por otro lado, se llev\u00f3 a cabo un estudio filogen\u00e9tico sobre una serie de clones biol\u00f3gicos recombinantes aislados a partir de los sobrenadantes de dos d\u00edas diferentes de un cultivo. Los resultados de este estudio ponen de manifiesto las consecuencias evolutivas que introduce la recombinaci\u00f3n en una poblaci\u00f3n dada: selecci\u00f3n de la combinaci\u00f3n de prote\u00ednas m\u00e1s favorables, numerosos eventos de recombinaci\u00f3n independientes, y selecci\u00f3n positiva de algunos clones. La poblaci\u00f3n viral obtenida tras 22 d\u00edas de cultivo, se caracteriz\u00f3 por ser m\u00e1s heterog\u00e9nea y disminuy\u00f3 la distancia gen\u00e9tica del conjunto.  por \u00faltimo, se realiz\u00f3 un estudio sobre la naturaleza diploide o pseudodiploide del vih, o sobre el origen<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Recombinaci\u00f3n del v.i.h. in vitro<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Recombinaci\u00f3n del v.i.h. in vitro <\/li>\n<li><strong>Autor:<\/strong>\u00a0 M. Jos\u00e9 Iglesias Sanchez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Complutense de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 09\/05\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Cecilio L\u00f3pez Gal\u00edndez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: agustin gregorio Zapata gonzalez <\/li>\n<li>Juan Ortin monton (vocal)<\/li>\n<li>cristina Escarmis oms (vocal)<\/li>\n<li>Rafael Fern\u00e1ndez mu\u00f1oz (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de M. Jos\u00e9 Iglesias Sanchez Este trabajo se ha centrado en el estudio de la influencia que ejerce [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[1],"tags":[6521,5646,68078,14725,68077,38633],"class_list":["post-22858","post","type-post","status-publish","format-standard","hentry","category-sin-categoria","tag-agustin-gregorio-zapata-gonzalez","tag-cecilio-lopez-galindez","tag-cristina-escarmis-oms","tag-juan-ortin-monton","tag-m-jose-iglesias-sanchez","tag-rafael-fernandez-munoz"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/22858","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=22858"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/22858\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=22858"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=22858"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=22858"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}