{"id":23273,"date":"2003-03-06T00:00:00","date_gmt":"2003-03-06T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/aislamiento-purificacion-y-caracterizacion-de-las-protea%c2%adnas-capaces-de-ligar-los-acidos-grasos-en-leishmania-donovani\/"},"modified":"2003-03-06T00:00:00","modified_gmt":"2003-03-06T00:00:00","slug":"aislamiento-purificacion-y-caracterizacion-de-las-protea%c2%adnas-capaces-de-ligar-los-acidos-grasos-en-leishmania-donovani","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/zoologia\/aislamiento-purificacion-y-caracterizacion-de-las-protea%c2%adnas-capaces-de-ligar-los-acidos-grasos-en-leishmania-donovani\/","title":{"rendered":"Aislamiento, purificaci\u00f3n y caracterizaci\u00f3n de las prote\u00ednas capaces de ligar los \u00e1cidos grasos en leishmania donovani"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Mimoun Maache <\/strong><\/h2>\n<p>En esta tesis se ha comprobado la presencia de las prote\u00ednas ligadoras de \u00e1cidos grasos (fabp) en leishmania donovani. Un tema de gran actualidad, debido a su posible uso en el diagn\u00f3stico y como vacuno contra las enfermedades causadas por par\u00e1sitos. As\u00ed como la posible intervenci\u00f3n de estas prote\u00ednas en un tratamiento selectivo dada la incapacidad de s\u00edntesis de novo de \u00e1cidos grasos por parte de estos organismos.  se ha llevado a cabo este trabajo con la forma promastigote del par\u00e1sito y mediante cromatograf\u00eda de afinidad se ha conseguido purificar las fabps de leishmania (leish-fabp) caracteriz\u00e1ndose mediante t\u00e9cnicas electrofor\u00e9ticas en sds-page y en ief se determin\u00f3 su peso molecular y su punto isoel\u00e9ctrico (pi). Las reacciones positivas demostradas frente a varios sueros con leishmaniosis de origen humano y animal y la inexistencia de reacciones cruzadas frente a otros microorganismos intracelulares (trypanosoma cruzi, toxoplasmosis, s\u00edfilis, tuberculosis) nos induce a considerar a estas prote\u00ednas como ant\u00edgenos espec\u00edficos en el diagn\u00f3stico de la leishmaniosis.  la creacci\u00f3n de anticuerpos (anti-fabp) en ratones ha permitido utilizar varias t\u00e9cnicas de inmunolocalizaci\u00f3n como la inmunofluorescencia y la inmunocitoqu\u00edmica por las cuales se ha comprobado la amplia distribuci\u00f3n de la prote\u00ednas en el par\u00e1sito.  para determinar el fragmento inmunog\u00e9nico se ha tratado la prote\u00edna con varias enzimas como proteinasaa y algunos reactivos qu\u00edmicos como n-chlorosuccinamida, mostrando por lo menos dos fragmentos antig\u00e9nicos.  para comprobar la capacidad de la prote\u00edna en inducir respuesta inmunitaria y estudiar el tipo de la respuesta inducida se ha estudiado en varios lotes de ratones balb\/c (infectados y previamente inmunizados, infectados y sanos) estudi\u00e1ndose tanto los niveles de inmunoglobulinas (igg1, igg2 y igg3) como los niveles de citoquinas (il-2, il-4, il-10 y ifn-gamma) comprob\u00e1ndose que los<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Aislamiento, purificaci\u00f3n y caracterizaci\u00f3n de las prote\u00ednas capaces de ligar los \u00e1cidos grasos en leishmania donovani<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Aislamiento, purificaci\u00f3n y caracterizaci\u00f3n de las prote\u00ednas capaces de ligar los \u00e1cidos grasos en leishmania donovani <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Mimoun Maache <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Granada<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 03\/06\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Antonio Osuna Carrillo De Albornoz<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: george Hillyer <\/li>\n<li>Fernando Valdivieso amate (vocal)<\/li>\n<li> Espino hern\u00e1ndez Ana margarita (vocal)<\/li>\n<li>Antonio Martinez fern\u00e1ndez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Mimoun Maache En esta tesis se ha comprobado la presencia de las prote\u00ednas ligadoras de \u00e1cidos grasos [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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