{"id":23282,"date":"2003-03-06T00:00:00","date_gmt":"2003-03-06T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/identificacion-clonaje-y-caracterizacion-de-dio-1-un-nuevo-gen-implicado-en-apoptosis\/"},"modified":"2003-03-06T00:00:00","modified_gmt":"2003-03-06T00:00:00","slug":"identificacion-clonaje-y-caracterizacion-de-dio-1-un-nuevo-gen-implicado-en-apoptosis","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/identificacion-clonaje-y-caracterizacion-de-dio-1-un-nuevo-gen-implicado-en-apoptosis\/","title":{"rendered":"Identificaci\u00f3n, clonaje y caracterizaci\u00f3n de dio-1, un nuevo gen implicado en apoptosis"},"content":{"rendered":"<h2>Tesis doctoral de <strong> David Garc\u00eda Domingo <\/strong><\/h2>\n<p>La apoptosis o muerte celular programada es un fen\u00f3meno fisiol\u00f3gico de crucial importancia para el normal desarrollo y homeostasis de los organismos multicelulares. El gen dio-1 (death inducer-obliterator 1) fue identificado por nosotros en la l\u00ednea celular pre-bi wol-1, mediante la t\u00e9cnica de clonaje diferencial de genes conocida como \u00abdifferential display pcr\u00bb, tras el est\u00edmulo apopt\u00f3tico de deprivaci\u00f3n de il-7. Su secuencia proteica presenta homolog\u00edas con dominios de activaci\u00f3n transcripcional, una secuencia de localizaci\u00f3n nuclear de localizaci\u00f3n nuclear bipartita, un dedo de zinc tipo phd y una regi\u00f3n rica en lisinas en el extremo carboxilo terminal. tras ciertos est\u00edmulos apopt\u00f3ticos apropiados, sus niveles de arnm y prote\u00edna aumentan muy poco tiempo despu\u00e9s de la presencia del est\u00edmulo, observ\u00e1ndose que para ello precisa de su translocaci\u00f3n nuclear. Su sobreexpresi\u00f3n en diferentes l\u00edneas celulares, as\u00ed como en primordios de extremidades de pollo, indujo apoptosis, inhibible por bcl-2 o z-vad-fmk. La generaci\u00f3n de un mutante carente de las se\u00f1as de localizaci\u00f3n nuclear permiti\u00f3 descubrir su incapacidad para migrar al n\u00facleo tras el est\u00edmulo apopt\u00f3tico, incluso en condiciones que provocaron la translocaci\u00f3n y aumento de los niveles de dio-1. Puesto que la muerte inducida por dio-1 era inhibible por inhibidores de caspasas, quisimos averiguar la relaci\u00f3n entre dio-1 y las caspasas. para ello, analizamos los niveles de las procaspasas 3 y 9 y de sus actividades tras la expresi\u00f3n de dio-1 y del mutante sin nls en c\u00e9lulas fl5.12. Encontramos que dio-1 aumenta los niveles de dichas procaspasas y las actividades de sus formas activas, mientras que el mutante fue incapaz de producir este efecto, lo que sugiere que dio-1 precisa de su translocaci\u00f3n al n\u00facleo para aumentar los niveles de caspasas. Adem\u00e1s, c\u00e9lulas fl5.12 que expresaban establemente el mutante se vieron protegidas frente a la apoptosis inducid<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Identificaci\u00f3n, clonaje y caracterizaci\u00f3n de dio-1, un nuevo gen implicado en apoptosis<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Identificaci\u00f3n, clonaje y caracterizaci\u00f3n de dio-1, un nuevo gen implicado en apoptosis <\/li>\n<li><strong>Autor:<\/strong>\u00a0 David Garc\u00eda Domingo <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 03\/06\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Carlos Martinez Alonso<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal:  Moreno de albor\u00e1n vierna ignacio (secretario) <\/li>\n<li>balbino Jos\u00e9 Alarcon sanchez (vocal)<\/li>\n<li>Manuel Izquierdo pastor (vocal)<\/li>\n<li>  (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de David Garc\u00eda Domingo La apoptosis o muerte celular programada es un fen\u00f3meno fisiol\u00f3gico de crucial importancia para [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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