{"id":23484,"date":"2003-12-06T00:00:00","date_gmt":"2003-12-06T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-bioquimica-y-molecular-del-metabolismo-del-acido-pipecolico-en-penicillium-chrysogenum\/"},"modified":"2003-12-06T00:00:00","modified_gmt":"2003-12-06T00:00:00","slug":"caracterizacion-bioquimica-y-molecular-del-metabolismo-del-acido-pipecolico-en-penicillium-chrysogenum","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/leon\/caracterizacion-bioquimica-y-molecular-del-metabolismo-del-acido-pipecolico-en-penicillium-chrysogenum\/","title":{"rendered":"Caracterizacion bioquimica y molecular del metabolismo del acido pipecolico en penicillium chrysogenum"},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Naranjo Brice\u00f1o Leopoldo Antonio <\/strong><\/h2>\n<p>El \u00e1cido pipec\u00f3lico es un componente y un precursor de diversos metabolitos secundarios con importantes actividades farmacol\u00f3gicas. En primer lugar, en el presente trabajo de investigaci\u00f3n, se demostr\u00f3 que penicillium chrysogenum es capaz de u tilizar \u00e1cido pipec\u00f3lico para complementar los requermientos nutricionales de varios mutantes aux\u00f3trofos de lisina (lys-). La clonaci\u00f3n del gen que codifica la sacaropina reductasa en p. Chrysogenum (denominado gen lys7), as\u00ed como la caracterizaci\u00f3n bioqu\u00edmica y molecular de mutantes aux\u00f3trofos de lisina que eran incapaces de utilizar \u00e1cido pipec\u00f3lico para complementar dicha auxotrofia (lys-\/pip-), permiti\u00f3 determinar que en este hongo filamentoso, el \u00e1cido pipec\u00f3lico se convierte en lisina a trav\u00e9s de su conversi\u00f3n en \u00e1cido piperid\u00edn-6-carbox\u00edlico (p6c) por acci\u00f3n de la pipecolato oxidasa, el p6c (que es la forma ciclada de alfa-aminoadipato gamma-semialdeh\u00eddo) se convierte en sacaropina y lisina a trav\u00e9s de dos reacciones enzim\u00e1ticas consecutivas catalizadas por la sacaropina reductasa y la sacaropina deshidrogenasa respectivamente.  en segundo lugar, se demostr\u00f3 que penicillium crhysogenum es capaz de sintetizar \u00e1cido pipec\u00f3lico. El gen lys7 que codifica la sacaropina reductasa en p. crhysogenum fue interrumpido por la t\u00e9cnica de la doble recombinaci\u00f3n. la cepa interrumpida (denominada p. Chrysogenum sr1-) carece de actividad sacaropina reductasa, la cual, se recupera despu\u00e9s de la transformaci\u00f3n de dicho mutante con el gen lys7 intacto presente en un pl\u00e1smido de replicaci\u00f3n auton\u00f3ma. La cepa p. Chrysogenum sr1- es aux\u00f3trofa de lisina y acumula p6c. Cuando el mutante p. Chrysogenum sr1- se crece con l-lisina como \u00fanica fuente de nitr\u00f3geno y se a\u00f1ade \u00e1cido d,l-alfa-aminoad\u00edpico al medio de cultivo, se acumulaban intracelularmente niveles altos de  \u00e1cido pipec\u00f3lico. la comparaci\u00f3n de la cepa sr1- con una cepainterrumpida en el gen lys2 que codifica la al<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizacion bioquimica y molecular del metabolismo del acido pipecolico en penicillium chrysogenum<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizacion bioquimica y molecular del metabolismo del acido pipecolico en penicillium chrysogenum <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Naranjo Brice\u00f1o Leopoldo Antonio <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Le\u00f3n<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 12\/06\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Juan  Francisco Mart\u00edn Mart\u00edn<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: arturo P\u00e9rez eslava <\/li>\n<li>oscar Ba\u00f1uelos hortiguela (vocal)<\/li>\n<li>Jos\u00e9 Luis Garcia lopez (vocal)<\/li>\n<li>\u00e1ngel Dom\u00ednguez olavarri (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Naranjo Brice\u00f1o Leopoldo Antonio El \u00e1cido pipec\u00f3lico es un componente y un precursor de diversos metabolitos secundarios [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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