{"id":23768,"date":"2018-03-09T09:14:27","date_gmt":"2018-03-09T09:14:27","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/aproximacion-a-la-caracterizacion-funcional-y-estructural-del-tallo-ribosomico-de-saccharomyces-cerevisiae\/"},"modified":"2018-03-09T09:14:27","modified_gmt":"2018-03-09T09:14:27","slug":"aproximacion-a-la-caracterizacion-funcional-y-estructural-del-tallo-ribosomico-de-saccharomyces-cerevisiae","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/aproximacion-a-la-caracterizacion-funcional-y-estructural-del-tallo-ribosomico-de-saccharomyces-cerevisiae\/","title":{"rendered":"Aproximaci\u00f3n a la caracterizaci\u00f3n funcional y estructural del tallo ribos\u00f3mico de saccharomyces cerevisiae"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Jorge P\u00e9rez Fern\u00e1ndez <\/strong><\/h2>\n<p>El tallo ribos\u00f3mico constituye un dominio funcional muy din\u00e1mica y a\u00fan muy poco conocida. El tallo de s.Cerevisiae est\u00e1 constitu\u00eddo de cuatro prote\u00ednas de 12 kda (p1alfa, p1beta, p2alfa and p2beta), que forma un pent\u00e1mero con la prote\u00edna p0. Las proteinas \u00e1cidas unidas a ribosoma se intercambian con las presentes en un reservorio citopl\u00e1smico durante la s\u00edntesis de prote\u00ednas, y est\u00e1n probablemente implicadas en un proceso de regulaci\u00f3n de la traducci\u00f3n.  las interacciones de las diferentes prote\u00ednas \u00e1cidas con p0, implicadas en el proceso de ensamblaje del tallo han sido exploradas empleando el sistema de doble h\u00edbrido, cromatograf\u00eda de afinidad, an\u00e1lisis de mutantes de delecci\u00f3n y mutantes puntuales.  los resultados indican que la prote\u00edna p0 completa interacciona debilmente con las prote\u00ednas p1. En contraste, un fragmento de 100 amino\u00e1cidos de largo une de forma muy fuerte las prote\u00ednas p1, con menor intensidad la prote\u00edna p2alfa, y no interacciona con la prote\u00edna p2beta. La delecci\u00f3n progresiva de ambos extremos a permitido definir un fragmento de las posiciones 212 a 265 como la parte m\u00e1s peque\u00f1a de p0 capaz de interaccionar con las prote\u00ednas. Dem\u00e1s, los resultados sugieren la participaci\u00f3n del extremos carboxilo en la interacci\u00f3n espec\u00edfica de las prote\u00ednas p2. La delecci\u00f3n de 15 amino\u00e1cidos de p0 resulta en una reducci\u00f3 dr\u00e1stica de las prote\u00ednas \u00e1cidas unidas a ribosoma. Los an\u00e1lisis de mutaciones puntuales descartan la interacci\u00f3n por cremalleras de leucinas como el mecanismo de interacci\u00f3n relevante. Los resultados sostienen un modelo de ensamblaje del tallo que requiere un cambio conformacional de p0 para exponer los sitios de uni\u00f3n de las prote\u00ednas p1, que es seguido por la asociaci\u00f3n de las prote\u00ednas p2 al complejo.  la interacci\u00f3n de esta estructura con varios factores de traducci\u00f3n ha sido tambi\u00e9n caracterizada por diferentes m\u00e9todos. Hemos aplicado el sistema de doble h<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Aproximaci\u00f3n a la caracterizaci\u00f3n funcional y estructural del tallo ribos\u00f3mico de saccharomyces cerevisiae<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Aproximaci\u00f3n a la caracterizaci\u00f3n funcional y estructural del tallo ribos\u00f3mico de saccharomyces cerevisiae <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Jorge P\u00e9rez Fern\u00e1ndez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 20\/06\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Juan  Pedro Garc\u00eda Ballesta<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Antonio Jimenez-Martinez <\/li>\n<li>alonso Rodriguez navarro (vocal)<\/li>\n<li>Francisco Portillo perez (vocal)<\/li>\n<li>isabel L\u00f3pez calder\u00f3n (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Jorge P\u00e9rez Fern\u00e1ndez El tallo ribos\u00f3mico constituye un dominio funcional muy din\u00e1mica y a\u00fan muy poco conocida. [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[1],"tags":[540,9593,7553,9594,70286,14711],"class_list":["post-23768","post","type-post","status-publish","format-standard","hentry","category-sin-categoria","tag-alonso-rodriguez-navarro","tag-antonio-jimenez-Martinez","tag-francisco-portillo-perez","tag-isabel-lopez-calderon","tag-jorge-perez-fernandez","tag-juan-pedro-garcia-ballesta"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/23768","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=23768"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/23768\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=23768"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=23768"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=23768"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}