{"id":23916,"date":"2018-03-09T09:14:39","date_gmt":"2018-03-09T09:14:39","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/el-gen-carps0-en-la-identificacion-de-candida-albicans-y-otras-especies-fungicas-mediante-pcr-y-clonacion-del-gen-homologo-en-candida-tropicalis\/"},"modified":"2018-03-09T09:14:39","modified_gmt":"2018-03-09T09:14:39","slug":"el-gen-carps0-en-la-identificacion-de-candida-albicans-y-otras-especies-fungicas-mediante-pcr-y-clonacion-del-gen-homologo-en-candida-tropicalis","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/el-gen-carps0-en-la-identificacion-de-candida-albicans-y-otras-especies-fungicas-mediante-pcr-y-clonacion-del-gen-homologo-en-candida-tropicalis\/","title":{"rendered":"El gen carps0 en la identificaci\u00f3n de candida albicans y otras especies fungicas mediante pcr y clonaci\u00f3n del gen hom\u00f3logo en candida tropicalis"},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Baquero Herrera Claudia Janneth <\/strong><\/h2>\n<p>A partir de la secuencia nucleot\u00eddica del intr\u00f3n del gen carps0 de candida albicans se ha dise\u00f1ado una pareja de oligonucle\u00f3tidos cebadores que se ha empleado en un an\u00e1lisis de pcr sobre dna gen\u00f3mico y c\u00e9lulas enteras de especies del g\u00e9nero candida y otros microorganismos. En todos los aislados cl\u00ednicos de c. Albicans se gener\u00f3 el amplic\u00f3n de 310 pb esperado; en otroas especies de candida, as\u00ed como en otras cepas de laboratorio pertenecientes a otros hongos, no se gener\u00f3 ning\u00fan amplic\u00f3n excepto en los casos de c.Pseudotropicalis (amplic\u00f3n de 1200 pb), kluyveromices marxianus (amplic\u00f3n de 1250 pb) y cryptococcus neoformans (varios amplicons mayores de 1250 pb). En aislados poco comunes de c.Albicans procedentes de africa tambi\u00e9n se gener\u00f3 el albic\u00f3n de 310 pb esperado. Estos resultados indican que los genes que presentan intrones pueden ser de utilidad en el dise\u00f1o de oligonucle\u00f3tidos cebadores, espec\u00edficos de especie, para la identificaci\u00f3n mediante pcr de cepas f\u00fangicas. la sensibilidad del m\u00e9todo se evalu\u00f3 empleando diferentes cantidades (de 224 ng a 2.7 pg) e dna de c.Albicans as\u00ed como de distintas cantidades de c\u00e9lulas (de 10(7) a 5). Los resultados obtenidos pueden ser \u00fatiles en una detecci\u00f3n temprana de candidiasis.  los genes rps0 a y b de saccharomyces cerevisiae codifican prote\u00ednas esenciales para la maduraci\u00f3n de la subunidad ribosomal 40s. Hemos aislado el gen hom\u00f3logo a rps0 en c.Tropicalis y lo hemos denominado ctrps0. El gen ctrps0 codifica para una prote\u00edna de 261 res\u00edduos aminoac\u00eddicos con un tama\u00f1o molecular te\u00f3rico de 28.65 kda y un punto isoel\u00e9ctrico de 4.79. La prote\u00edna ctrsp0 presenta una elevada homolog\u00eda con c.Albicans, s.Cerevisiae, neurospora crassa, schizosaccharaomyces pombe, pneumocystis carinni y otros organismos como humanos, rat\u00f3n y rata. Ctrps0, en un vector de elevado n\u00famero de copias, es capaz de complementar el fenotipo letal unido a la disrupci\u00f3n de los dos ge<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>El gen carps0 en la identificaci\u00f3n de candida albicans y otras especies fungicas mediante pcr y clonaci\u00f3n del gen hom\u00f3logo en candida tropicalis<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 El gen carps0 en la identificaci\u00f3n de candida albicans y otras especies fungicas mediante pcr y clonaci\u00f3n del gen hom\u00f3logo en candida tropicalis <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Baquero Herrera Claudia Janneth <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Universitat de val\u00e9ncia (estudi general)<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 25\/06\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Eulogio Valent\u00edn G\u00f3mez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: federico Uruburu fern\u00e1ndez <\/li>\n<li> Balaguer mart\u00ednez Jos\u00e9 vicente (vocal)<\/li>\n<li>Juan  Carlos Arguelles ordo\u00f1ez (vocal)<\/li>\n<li>Mar\u00eda francisca Colom valiente (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Baquero Herrera Claudia Janneth A partir de la secuencia nucleot\u00eddica del intr\u00f3n del gen carps0 de candida [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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