{"id":23971,"date":"2018-03-09T09:14:43","date_gmt":"2018-03-09T09:14:43","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/identificacion-y-caracterizacion-de-la-caja-sos-de-ralstonia-metallidurans-y-deinococcus-radiodurans\/"},"modified":"2018-03-09T09:14:43","modified_gmt":"2018-03-09T09:14:43","slug":"identificacion-y-caracterizacion-de-la-caja-sos-de-ralstonia-metallidurans-y-deinococcus-radiodurans","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/identificacion-y-caracterizacion-de-la-caja-sos-de-ralstonia-metallidurans-y-deinococcus-radiodurans\/","title":{"rendered":"Identificaci\u00f3n y caracterizaci\u00f3n de la caja sos de ralstonia metallidurans y deinococcus radiodurans"},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Casares Proa\u00f1o Lorena Cecilia <\/strong><\/h2>\n<p>El sistema sos de escherichia coli ha sido durante mucho tiempo el modelo de referencia para su estudio en otras especies. Este sistema se encuentra en otros microorganismos incluyendo bacterias gramnegativas, grampositivas y otras. Recientemente se ha encontrado diferencias entre las cajas sos y los genes que integran el regul\u00f3n sos en e.Coli respecto a otras especies bacterianas.  el prop\u00f3sito de este trabajo ha sido determinar y caracterizar las cajas sos de dos especies bacterianas, ralstonia metallidurans y deinococcus radiodurans, pertenecientes a los grupos beta-progeobacterias y deinococcus-thermus, respectivamente.  en primer lugar se realiz\u00f3 la clonaci\u00f3n y secuenciaci\u00f3n de los genes reca y lexa de r.Metallidurans, el primero mediante hibridaci\u00f3n con una sonda del gen reca de agrobacterium tumefaciens y el segundo utlizando programas inform\u00e1ticos en los que se us\u00f3 la secuencia del gen lexa de e.Coli para identificar dicho gen en la secuencia parcial del genoma de r.Metallidurans. tras un an\u00e1lisis de sus regiones promotoras, se determin\u00f3 que ambas conten\u00edan un motivo regulador, ctgt-n8-acag, id\u00e9ntico al de e.Coli.  se comprob\u00f3 que esta caja reguladora era funcional tanto en r.Metallidurans como e.Coli, evaluando su capacidad de inducir el gen reca frente a lesiones en el dna. Adicionalmente se determin\u00f3 que la caja sos de este microorganismo se encontraba en varios genes que, en e.Coli forman parte del regul\u00f3n sos, como reca, lexa y un hipot\u00e9tico gen de la familia impb\/samb\/mucb. En cambio, no se identific\u00f3 dicha caja en los hipot\u00e9ticos genes uvra, ruvab y ding, los cuales, en e.Coli, tambi\u00e9n integran el regul\u00f3n sos. Mediante el an\u00e1lisis por rt-pcr on-line de los tr\u00e1nscritos se demostr\u00f3 que la expresi\u00f3n de todos estos genes era inducible al lesionar el dna. Asimismo, mediante ensayos de movilidad electrofor\u00e9tica, utilizando extractos proteicos de lexa de e.Coli purificada y extractos crudos de r.Metallid<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Identificaci\u00f3n y caracterizaci\u00f3n de la caja sos de ralstonia metallidurans y deinococcus radiodurans<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Identificaci\u00f3n y caracterizaci\u00f3n de la caja sos de ralstonia metallidurans y deinococcus radiodurans <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Casares Proa\u00f1o Lorena Cecilia <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de barcelona<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 26\/06\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Monteserrat Llagostera Casas<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: ernesto Garc\u00eda l\u00f3pez <\/li>\n<li>ferran Navarro risue\u00f1o (vocal)<\/li>\n<li>Miguel Regu\u00e9 queralt (vocal)<\/li>\n<li> Rey p\u00e9rez alfonso del (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Casares Proa\u00f1o Lorena Cecilia El sistema sos de escherichia coli ha sido durante mucho tiempo el modelo [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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