{"id":24075,"date":"2018-03-09T09:14:52","date_gmt":"2018-03-09T09:14:52","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/evolucion-del-virus-de-la-fiebre-aftosa-hacia-genomas-defectivos-que-son-infectivos-por-complementacion\/"},"modified":"2018-03-09T09:14:52","modified_gmt":"2018-03-09T09:14:52","slug":"evolucion-del-virus-de-la-fiebre-aftosa-hacia-genomas-defectivos-que-son-infectivos-por-complementacion","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/evolucion-del-virus-de-la-fiebre-aftosa-hacia-genomas-defectivos-que-son-infectivos-por-complementacion\/","title":{"rendered":"Evoluci\u00f3n del virus de la fiebre aftosa hacia genomas defectivos que son infectivos por complementaci\u00f3n"},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Garc\u00eda Arriaza Juan  Francisco <\/strong><\/h2>\n<p>En esta tesis doctoral se ha documentado la selecci\u00f3n de variantes virales del virus de la fiebre aftosa (vfa) c3arg\/85 despu\u00e9s de un solo proceso de infecci\u00f3n en c\u00e9lulas bhk-21 parcialmente resistentes, procedentes de c\u00e9lulas persitentemente infectadas, en las que ha sido eliminado el vfa mediante tratamiento con ribavirina (c\u00e9lulas denominadas bhk-rb). Tras 8-10 d\u00edas post-infecci\u00f3n se liberaron virus variantes, denominados virus c3-rb. Los virus c3-rb seleccionados mostraron un incremento de virulencia para c\u00e9lulas bhk-21, fueron capaces de superar la resistencia a la infecci\u00f3n por parte de las c\u00e9lulas bhk-21 modificadas (bhk-rb) y adquirieron la capacidad de unir heparina e infectar c\u00e9lulas cho. Una comparaci\u00f3n de las secuencias gen\u00f3micas completas del virus parental c3arg\/85 y de los virus variantes c3-rb, revel\u00f3 solo dos cambios de amino\u00e1cido (asp-9 ala en vp3 y o bien gly-110 arg o his108 arg en vp1), localizados en la superficie de la part\u00edcula viral, en el eje de simetr\u00eda de orden 5, representando un aumento de cargas positivas en la superficie del viri\u00f3n.  tambi\u00e9n se ha documentado, por primera vez, la generaci\u00f3n y caracterizaci\u00f3n de part\u00edculas defectivas interferentes que complementan entre s\u00ed, obtenidas tras 260 pases seriados del clon de vfa c-s8c1 a alta  multiplicidad de infecci\u00f3n en c\u00e9lulas bhk-21. Se ha estudiado una poblaci\u00f3n, denominada c-s8p260, rica en genomas defectivos, que muestra niveles indetectables de genomas de tama\u00f1o est\u00e1ndar (los genomas defectivos son, al menos, 10000 veces m\u00e1s abundantes que los genomas de tama\u00f1o est\u00e1ndar). El an\u00e1lisis de secuencia revel\u00f3 que esta poblaci\u00f3n contiene tres diferentes rnas defectivos que incluyen deleciones dentro de la regi\u00f3n codificante de la proteasa l y de la regi\u00f3n codificante de las prote\u00ednas de la c\u00e1psula, y retienen el marco de lectura abierto del genoma viral. Un an\u00e1lisis mediante gradiente de sacarosa y microscopia electr\u00f3nica<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Evoluci\u00f3n del virus de la fiebre aftosa hacia genomas defectivos que son infectivos por complementaci\u00f3n<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Evoluci\u00f3n del virus de la fiebre aftosa hacia genomas defectivos que son infectivos por complementaci\u00f3n <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Garc\u00eda Arriaza Juan  Francisco <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 27\/06\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Esteban Domingo Solans<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: jose Fernandez piqueras <\/li>\n<li>albert Bosch navarro (vocal)<\/li>\n<li>Jos\u00e9 Mar\u00eda Almendral del rio (vocal)<\/li>\n<li>  (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Garc\u00eda Arriaza Juan Francisco En esta tesis doctoral se ha documentado la selecci\u00f3n de variantes virales del [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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