{"id":24217,"date":"2003-02-07T00:00:00","date_gmt":"2003-02-07T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/insercion-de-fluorocromos-en-el-gen-env-de-vih-1-para-el-analisis-de-resistencias-fenota%c2%adpicas-a-antirretrovirales\/"},"modified":"2003-02-07T00:00:00","modified_gmt":"2003-02-07T00:00:00","slug":"insercion-de-fluorocromos-en-el-gen-env-de-vih-1-para-el-analisis-de-resistencias-fenota%c2%adpicas-a-antirretrovirales","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-medicas\/insercion-de-fluorocromos-en-el-gen-env-de-vih-1-para-el-analisis-de-resistencias-fenota%c2%adpicas-a-antirretrovirales\/","title":{"rendered":"Inserci\u00f3n de fluorocromos en el gen env de vih-1 para el an\u00e1lisis de resistencias fenot\u00edpicas a antirretrovirales"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Alicia Vela Boza <\/strong><\/h2>\n<p>El an\u00e1lisis de resistencia a antirretrovirales puede realizarse por t\u00e9cnicas de genotipado y de fenotipado. Estas \u00faltimas utilizan m\u00e9todos biol\u00f3gicos complejos, de no muy alta eficacia y limitada seguridad. Kellam, p., Y larder, b.A. (1994) desarrollaron m\u00e9todos utilizando virus recombinantes y boucher, c.A., Y cols. (1996) han desarrollado esta metodolog\u00eda utilizando el ensayo con mtt tratando de simplificar y ajustar el procedimiento para usarlo con virus recombinantes generados a partir del suero de pacientes.  la adaptaci\u00f3n de la prote\u00edna verde fluorescente de aequorea victoria (gfp) a t\u00e9cnicas de ingenier\u00eda gen\u00e9tica y citometr\u00eda de flujo, supone una importante alternativa en las construcciones que pueden utilizarse para el trabajo con retrovirus. De este modo creemos en la posibilidad de lograr un dise\u00f1o experimental ajustado que permita la expresi\u00f3n a t\u00edtulos detectables de gfp y de esa forma mejorar la cuantificaci\u00f3n de los efectos fenot\u00edpicos de las construcciones de vih-1.  el objetivo de este trabajo fue la construcci\u00f3n de vectores basados en virus de la inmunodeficiencia humana (vih-1) para poder:  i,- desarrollar modelos adecuados de valoraci\u00f3n de resistencias fenot\u00edpicas a antirretrovirales.  ii,- construir pseudotipos basados en vih-1 que mejoren los t\u00edtulos de virus obtenidos, el rango de c\u00e9lulas hospedadoras y la seguridad.  el vector original para este trabajo es el pnl4.3 y como sistema marcador hemos utilizado la prote\u00edna fluorescente (egfp) cuya expresi\u00f3n hemos valorado por citometr\u00eda de flujo.  iii,- comparaci\u00f3n de la efectividad del marcador fluorescente en la c\u00e9lula o en el vector.  iv,- comparaci\u00f3n de la infecci\u00f3n en dos l\u00edneas celulares diferentes cem-ss y mt2.  conclusiones  1,- la inserci\u00f3n del gen egfp en el gen env de vih-1 genera vectores retrovirales infectivos y capaces de expresar la prote\u00edna verde como consecuencia de la replicaci\u00f3n viral al ser contrasfectados<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Inserci\u00f3n de fluorocromos en el gen env de vih-1 para el an\u00e1lisis de resistencias fenot\u00edpicas a antirretrovirales<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Inserci\u00f3n de fluorocromos en el gen env de vih-1 para el an\u00e1lisis de resistencias fenot\u00edpicas a antirretrovirales <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Alicia Vela Boza <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Santiago de compostela<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 02\/07\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Benito Regueiro Garcia<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jos\u00e9 angel Garc\u00eda rodr\u00edguez <\/li>\n<li>Antonio Aguilera guirao (vocal)<\/li>\n<li>Rafael Seoane prado (vocal)<\/li>\n<li>Juan  Jos\u00e9 Picazo de la garza (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Alicia Vela Boza El an\u00e1lisis de resistencia a antirretrovirales puede realizarse por t\u00e9cnicas de genotipado y de [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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