{"id":2445,"date":"1994-01-01T00:00:00","date_gmt":"1994-01-01T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/1994\/01\/01\/estudio-cromatografico-fluorimetrico-de-la-degradacion-enzimatica-de-diadenosin-polifosfatos-en-medula-adrenal-bovina\/"},"modified":"1994-01-01T00:00:00","modified_gmt":"1994-01-01T00:00:00","slug":"estudio-cromatografico-fluorimetrico-de-la-degradacion-enzimatica-de-diadenosin-polifosfatos-en-medula-adrenal-bovina","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/estudio-cromatografico-fluorimetrico-de-la-degradacion-enzimatica-de-diadenosin-polifosfatos-en-medula-adrenal-bovina\/","title":{"rendered":"Estudio cromatografico-fluorimetrico de la degradacion enzimatica de diadenosin polifosfatos en medula adrenal bovina."},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Ramos Carrillo Antonio  Manuel <\/strong><\/h2>\n<p>Se han preparado los compuestos fluorescentes di-etenoadenosina polifosfatos, epsilon-(apna) a partir de los precursores diadenosina polifosfatos, apna, por tratamiento con cloroacetaldehido. La hidrolisis enzimatica de los compuestos epsilon-(apna) produce un importante aumento de fluorescencia (ex.305 nm, em.410 nm) que depende del numero de grupos fosfato. Mediante hplc en fase reversa es posible separar todos los compuestos epsilon-(apna) n=2-6 y sus productos de degradacion enzimatica. Los compuestos epsilon-(apna) son excelentes sustratos de los enzimas con actividad dinucleosido polifosfato hidrolasa, especificas e inespecificas, presentes en tejido adrenocromafin.  las celulas cromafines contienen un ectoenzima de baja especificidad y alta afinidad hacia los sustratos epsilon-(apna) n=2-6 y cuyos productos son epsilon-amp y epsilon-ap(n-1); apna y gpng son sustratos alternativos, km 2 microm. El tejido adrenocromafin contiene al menos tres actividades citosolicas capaces de hidrolizar dinucleosido polifosfatos, uno inespecifico y dos de alta especificidad. El enzima inespecifico de masa mayor que 150 kda presenta un patron de corte analogo al del ectoenzima. Los enzimas altamente especificos de 30 y 20 kda respectivamente se corresponden con las actividades dinoclucleosido trifosfato hidrolasa y dinucleosido tetrafosfato hidrolasa y presentan km de 11 microm y 1 microm para los sustratos epsilon-(ap3a) y epsilon-(ap4a) respectivamente. Ambos enzimas especificos son inhibidos por iones zn2+ pero el enzima de 20 kda es fuertemente inhibido por el ion f- y los nucleotidos ap4 (ki=5 nm) epsilon-ap4 y epsilon-ap5.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Estudio cromatografico-fluorimetrico de la degradacion enzimatica de diadenosin polifosfatos en medula adrenal bovina.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Estudio cromatografico-fluorimetrico de la degradacion enzimatica de diadenosin polifosfatos en medula adrenal bovina. <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Ramos Carrillo Antonio  Manuel <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 La laguna<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 01\/01\/1994<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Pedro Rotllan Pascual<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Mar\u00eda  Teresa Miras Portugal <\/li>\n<li>Ana Mar\u00eda Afonso Perera (vocal)<\/li>\n<li>Venerando Gonzalez Diaz (vocal)<\/li>\n<li>Manuel Criado Herrero (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Ramos Carrillo Antonio Manuel Se han preparado los compuestos fluorescentes di-etenoadenosina polifosfatos, epsilon-(apna) a partir de los [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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