{"id":24669,"date":"2003-11-07T00:00:00","date_gmt":"2003-11-07T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/aislamiento-de-genes-de-la-sa%c2%adntesis-e-inactivacion-de-las-giberelinas-en-adelfa-y-estudios-de-sus-aplicaciones-biotecnologicas\/"},"modified":"2003-11-07T00:00:00","modified_gmt":"2003-11-07T00:00:00","slug":"aislamiento-de-genes-de-la-sa%c2%adntesis-e-inactivacion-de-las-giberelinas-en-adelfa-y-estudios-de-sus-aplicaciones-biotecnologicas","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/politecnica-de-valencia\/aislamiento-de-genes-de-la-sa%c2%adntesis-e-inactivacion-de-las-giberelinas-en-adelfa-y-estudios-de-sus-aplicaciones-biotecnologicas\/","title":{"rendered":"Aislamiento de genes de la s\u00edntesis e inactivaci\u00f3n de las giberelinas en adelfa y estudios de sus aplicaciones biotecnol\u00f3gicas"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Susana Ubeda  Tomas <\/strong><\/h2>\n<p>En este trabajo se ha estudiado el papel de las gas en el desarrollo de las plantas de nerium oleander (adelfa), a trav\u00e9s del estudio de los genes que codifican los enzimas implicados en el metabolismo de las gas.  para ello, hemos aislado y caracterizado las secuencias de cdna y las secuencias gen\u00f3micas de dos genes que codifican ga20oxidasas, enzimas implicados en la bios\u00edntesis de las gas en adelfa (noga20oxl y noga20ox2) y las secuencias de tres genes (noga2oxl, noga2ox2 y noga2ox3), que codifican ga2oxidasas, enzimas responsables de la inactivaci\u00f3n irreversible de las gas.  los ensayos enzim\u00e1ticos de las prote\u00ednas obtenidas tras la expresi\u00f3n heter\u00f3loga en escherichia coli de los clones de cdna aislados muestran que, las prote\u00ednas de fusi\u00f3n noga20ox1 y noga20ox2 convierten ga12 y ga53 en los productos ga9 y ga20 y, que la sprote\u00ednas noga2ox1, noga2ox2 y noga2ox3, convierten los substratos ga1, ga9 y ga20 en sus correspondientes productos 2beta-hidroxilados ga8, ga51 y ga29, respectivamente. Adem\u00e1s, la noga2ox3 cataliza dos oxidaciones consecutivas sobre ga9 y ga20 rindiendo ga51-catabolito y ga29-catabolito, respectivamente, caracter\u00edstica que le atribuye una naturaleza multifuncional a este enzima. Estos resultados demuestran que los clones de cdna noga20oxl y noga20ox2 codifican enzimas con actividad ga20oxidasa y, que los clones de cdna noga2oxl, noga2ox2 y noga2ox3 codifican enzimas con actividad ga2oxidasa, todos ellos enzimas funcionales in vitro.  la transformaci\u00f3n de nicotiana tabacum con el clon de cdna noga2ox3, bajo el control del promotor 35s, permite la expresi\u00f3n ect\u00f3pica de este gen y la obtenci\u00f3n de plantas de tabaco transg\u00e9nicas, las cuales poseen un d\u00e9ficit en gas activas. Los bajos niveles de gas activas en las plantas transg\u00e9nicas hace que distingamos dos tipos de fenotipos entre ellas: fenotipo enano (en las l\u00edneas l-40 y l-41) y fenotipo semienano (l-45.3, l-43.2 y l-46). Ambos fenotipos se d<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Aislamiento de genes de la s\u00edntesis e inactivaci\u00f3n de las giberelinas en adelfa y estudios de sus aplicaciones biotecnol\u00f3gicas<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Aislamiento de genes de la s\u00edntesis e inactivaci\u00f3n de las giberelinas en adelfa y estudios de sus aplicaciones biotecnol\u00f3gicas <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Susana Ubeda  Tomas <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Polit\u00e9cnica de Valencia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 11\/07\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Isabel Lopez Diaz<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: vicente Conejero tom\u00e1s <\/li>\n<li>esther Carrera bergua (vocal)<\/li>\n<li>Manuel Tal\u00f3n cubillo (vocal)<\/li>\n<li>Juan Carbonell gisbert (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Susana Ubeda Tomas En este trabajo se ha estudiado el papel de las gas en el desarrollo [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[16820],"tags":[72346,42464,16900,11526,72345,7881],"class_list":["post-24669","post","type-post","status-publish","format-standard","hentry","category-politecnica-de-valencia","tag-esther-carrera-bergua","tag-isabel-lopez-diaz","tag-juan-carbonell-gisbert","tag-manuel-talon-cubillo","tag-susana-ubeda-tomas","tag-vicente-conejero-tomas"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/24669","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=24669"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/24669\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=24669"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=24669"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=24669"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}