{"id":24802,"date":"2018-03-09T09:15:55","date_gmt":"2018-03-09T09:15:55","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-del-receptor-de-quimioquinas-ccr8-y-analisis-de-su-funcion-in-vivo\/"},"modified":"2018-03-09T09:15:55","modified_gmt":"2018-03-09T09:15:55","slug":"caracterizacion-del-receptor-de-quimioquinas-ccr8-y-analisis-de-su-funcion-in-vivo","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-del-receptor-de-quimioquinas-ccr8-y-analisis-de-su-funcion-in-vivo\/","title":{"rendered":"Caracterizaci\u00f3n del receptor de quimioquinas ccr8 y an\u00e1lisis de su funci\u00f3n in vivo"},"content":{"rendered":"<h2>Tesis doctoral de <strong> I\u00f1igo Goya Ayerra <\/strong><\/h2>\n<p>El trabajo experimental que se presenta en esta memoria versa acerca del mundo de las quimioquinas y sus receptores, y sobre la importancia que \u00e9stos tienen en la actividad leucocitaria. Nace, concretamente, de la caracterizaci\u00f3n que zaballos et al., Hicieron del receptor de quimioquinas inicialmente denominado ckr-l1 y que posteriormente se ha dado en llamar hccr8.  en primer lugar, y habiendo establecido que la quimioquina hccl1 interacciona con este receptor de forma espec\u00edfica, se clon\u00f3 su hom\u00f3logo en rat\u00f3n, mccr8, se\u00f1alando a mccl1 como su ligando, y situando la producci\u00f3n de su rna mensajero en c\u00e9lulas de timo, n\u00f3dulos linf\u00e1ticos y bazo. Se obtuvo, asimismo, un anticuerpo monoclonal anti-mccr8 con el que se advirti\u00f3, mediante citometr\u00eda de flujo, la presencia del receptor en la superficie de timocitos cd4- cd8- (dn), as\u00ed como en la de aquellos con fenotipo cd4+ (sp) que a\u00fan no han alcanzado su madurez. Tambi\u00e9n pudo concluirse que las c\u00e9lulas cd4- cd8- (dn) ccr8+ de timo son mayoritariamente tcrgama*+ y en menor medida tcrgama*+. Fue a trav\u00e9s de experimentos de rt-pcr cuantitativa llevados a cabo sobre ratones wild-type y de diversos an\u00e1lisis de citometr\u00eda efectuados con animales rag2(ko) como se clarific\u00f3 la expresi\u00f3n de ccr8 dentro de dicho comportamiento cd4- cd8- (dn) tcrgama*-, localiz\u00e1ndose \u00e9ta tanto en la subpoblaci\u00f3n cd44+ cd25- (dn1) como en la cd44- cd25- (dn4). Por rt-pcr se percibi\u00f3 adem\u00e1s la prsencia de ccr8 en c\u00e9lulas lin- sca-1+ de m\u00e9dula \u00f3sea. Del mismo modo, se corrobor\u00f3, en t\u00e9rminos de prote\u00edna, el dato ya defenido previamente en la literatura que habla del incremento transitotorio que, en linfocitos th2, experimentan los niveles de mrna de ccr8 en respuesta a activaci\u00f3n, vi\u00e9ndose que se produce el mismo efecto en timocitos cd4- cd8- (dn) y cd4+ (sp) tras la estimulaci\u00f3n del pre-tcr y el tcr respectivamente. Por otra parte, y con el \u00e1nimo de desentra\u00f1ar la actividad biol\u00f3gica at<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizaci\u00f3n del receptor de quimioquinas ccr8 y an\u00e1lisis de su funci\u00f3n in vivo<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizaci\u00f3n del receptor de quimioquinas ccr8 y an\u00e1lisis de su funci\u00f3n in vivo <\/li>\n<li><strong>Autor:<\/strong>\u00a0 I\u00f1igo Goya Ayerra <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 16\/07\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>\u00e1ngel Zaballos Sanz<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Francisco Sanchez Madrid <\/li>\n<li>Mar\u00eda  Luisa Toribio garcia (vocal)<\/li>\n<li>balbino Jos\u00e9 Alarcon sanchez (vocal)<\/li>\n<li>joaqu\u00edn Teixid\u00f3 calvo (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de I\u00f1igo Goya Ayerra El trabajo experimental que se presenta en esta memoria versa acerca del mundo de [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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