{"id":24835,"date":"2018-03-09T09:15:58","date_gmt":"2018-03-09T09:15:58","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/clonacion-y-caracterizacion-del-gen-ker1-de-candida-albicans\/"},"modified":"2018-03-09T09:15:58","modified_gmt":"2018-03-09T09:15:58","slug":"clonacion-y-caracterizacion-del-gen-ker1-de-candida-albicans","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/clonacion-y-caracterizacion-del-gen-ker1-de-candida-albicans\/","title":{"rendered":"Clonaci\u00f3n y caracterizaci\u00f3n del gen ker1 de candida albicans"},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Gal\u00e1n Albi\u00f1ana M. Amparo <\/strong><\/h2>\n<p>Candida albicans es un hongo pat\u00f3geno oportunista capaz de provocar infecciones a nivel superficial y sist\u00e9mico, provocando cifras de mortalidad de hasta el 35% en pacientes inmunodeprimidos.  entre los factores de virulencia de este hongo destaca el dimorfismo que es la capacidad que tiene el hongo de crecer tanto en forma levaduriforme como micelial.  en los \u00faltimos a\u00f1os, nuestro grupo ha desarrollado una l\u00ednea de investigaci\u00f3n encaminada a la caracterizaci\u00f3n de componentes celulares con el fen\u00f3meno del dimorfismo y de interacci\u00f3n del hongo con el hospedador. En este contexto, el principal objetivo de este trabajo consisti\u00f3 en el aislamiento y caracterizaci\u00f3n de un nuevo gen mediante elinmunorrastreo de una genoteca de expresi\u00f3n con un anticuerpo policlonal de la forma micelial del hongo (pab anti-gt). dicho gen est\u00e1 contenido en el contig 4-3030 (ahora 6-2517) del proyecto de secuenciaci\u00f3n del genoma de c.Albicans, carece de homolog\u00eda significativa con ninguna secuencia conocida, y contiene un marco abierto de lectura que codifica para una prote\u00edna de 1197 amino\u00e1cidos, con un peso molecular aproximado de 134 kda, y con un elevado porcentaje molar de lisina (14,5%) y \u00e1cido glut\u00e1mico )16,7%), por lo que se le ha denominado ker1p.  ensayos de inmunotransferencia de fracciones celulares con el anticuerpo pab anti-gt y con un anticuerpo policlonal inmunoespec\u00edfico frente a ker1p (pab anti-ker1p), revelaron que ker1p se localiza en la membrana plasm\u00e1tica de c.Albicans. Adem\u00e1s, el an\u00e1lisis de la expresi\u00f3n de ker1 revel\u00f3 que \u00e9ste se induce en c\u00e9lulas crecidas en los medios de lee y m199 a ph alcalino, queda reprimida a ph \u00e1cido y est\u00e1 regulado por rim101, el factor de transcripci\u00f3n que controla el dimorfismo y la expresi\u00f3n de genes dependientes de ph en este hongo.  el an\u00e1lisis fenot\u00edpico del mutante nulo ker1\/ker1, revel\u00f3 que ker1 debe estar implicado en la biog\u00e9nesis o composici\u00f3n de la pared celular de<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Clonaci\u00f3n y caracterizaci\u00f3n del gen ker1 de candida albicans<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Clonaci\u00f3n y caracterizaci\u00f3n del gen ker1 de candida albicans <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Gal\u00e1n Albi\u00f1ana M. Amparo <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Universitat de val\u00e9ncia (estudi general)<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 17\/07\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Jos\u00e9 Pedro Martinez Garcia<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: rafael Sentandreu ramon <\/li>\n<li>a.r. Gow neil (vocal)<\/li>\n<li>tom\u00e1s Gonz\u00e1lez villa (vocal)<\/li>\n<li>enrique Hernandez gimenez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Gal\u00e1n Albi\u00f1ana M. Amparo Candida albicans es un hongo pat\u00f3geno oportunista capaz de provocar infecciones a nivel [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[1],"tags":[72732,5580,72731,1259,9886,2553],"class_list":["post-24835","post","type-post","status-publish","format-standard","hentry","category-sin-categoria","tag-a-r-gow-neil","tag-enrique-hernandez-gimenez","tag-galan-albinana-m-amparo","tag-jose-pedro-Martinez-garcia","tag-rafael-sentandreu-ramon","tag-tomas-gonzalez-villa"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/24835","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=24835"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/24835\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=24835"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=24835"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=24835"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}