{"id":24856,"date":"2018-03-09T09:16:00","date_gmt":"2018-03-09T09:16:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/silenciamiento-de-genes-de-biosa%c2%adntesis-de-giberelinas-mediante-rna-antisentido-y-ribozimas-y-caracterizacion-del-mutante-hkt1-de-arabidopsis\/"},"modified":"2018-03-09T09:16:00","modified_gmt":"2018-03-09T09:16:00","slug":"silenciamiento-de-genes-de-biosa%c2%adntesis-de-giberelinas-mediante-rna-antisentido-y-ribozimas-y-caracterizacion-del-mutante-hkt1-de-arabidopsis","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/politecnica-de-valencia\/silenciamiento-de-genes-de-biosa%c2%adntesis-de-giberelinas-mediante-rna-antisentido-y-ribozimas-y-caracterizacion-del-mutante-hkt1-de-arabidopsis\/","title":{"rendered":"Silenciamiento de genes de bios\u00edntesis de giberelinas mediante rna antisentido y ribozimas y caracterizaci\u00f3n del mutante hkt1 de arabidopsis"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Jose P\u00e9rez G\u00f3mez <\/strong><\/h2>\n<p>Las giberelinas son sustancias reguladoras del crecimiento en plantas, y est\u00e1n implicadas en el control de la mayor\u00eda de las etapas del desarrollo de las mismas. La manipulaci\u00f3n gen\u00e9tica de la bios\u00edntesis de giberelinas en plantas es una herramienta muy \u00fatil para la comprensi\u00f3n del papel de estas fitohormonas en el desarrollo vegetal, as\u00ed como para la obtenci\u00f3n de mejoras en plantas cultivadas. En este trabajo, se ha estudiado el efecto de la manipulaci\u00f3n gen\u00e9tica de la ruta de bios\u00edntesis de gas  en  arabidopsis thaliana, a nivel de los genes atcps, que codifica una ent-copalil difosfato sintasa, y cuatro genes distintos que codifican ga 20-oxidasas. Para ello se han empleado ribozimas de cabeza de martillo, peque\u00f1os rnas con actividad catal\u00edtica capaces de unirse a un rna sustrato y cortarlo. En el caso del gen atcps tambi\u00e9n se ha empleado rna antisentido a modo de control. Se ha obtenido una planta transg\u00e9nica que expresa un fragmento del gen atcps en forma antisentido con niveles reducidos de expresi\u00f3n del gen atcps, lo que causa una disminuci\u00f3n del contenido en giberelinas activas de la planta, y un fenotipo semienano. En cambio, la expresi\u00f3n en plantas transg\u00e9nicas de ribozimas de cabeza de martillo dise\u00f1ados para cortar al gen atcps no provocaron una reducci\u00f3n en los niveles de expresi\u00f3n de atcps. En plantas transg\u00e9nicas que expresan ribozimas dise\u00f1ados frente a genes que codifican ga 20-oxidasas tampoco se observ\u00f3 una reducci\u00f3n de los niveles de transcritos de dichos genes, ni una alteraci\u00f3n de los caracteres fenot\u00edpicos controlados por gas. As\u00ed pues, la t\u00e9cnica de rna antisentido ha sido m\u00e1s eficaz que la de ribozimas de cabeza de martillo.  en este trabajo tambi\u00e9n se ha obtenido clones de cdna de los genes atga20ox4 y 5, que codifican ga 20-oxidasas. Se ha demostrado la actividad ga 20-oxidasa de su productos de expresi\u00f3n in vitro, y se ha visto que estos genes se expresan mayoritariamente en flo<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Silenciamiento de genes de bios\u00edntesis de giberelinas mediante rna antisentido y ribozimas y caracterizaci\u00f3n del mutante hkt1 de arabidopsis<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Silenciamiento de genes de bios\u00edntesis de giberelinas mediante rna antisentido y ribozimas y caracterizaci\u00f3n del mutante hkt1 de arabidopsis <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Jose P\u00e9rez G\u00f3mez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Polit\u00e9cnica de Valencia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 17\/07\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Jos\u00e9 Luis Garc\u00eda Mart\u00ednez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: ricardo Flores pedauye <\/li>\n<li>Jaime Martinez garcia (vocal)<\/li>\n<li> Bl\u00e1zquez rodr\u00edguez Miguel angel (vocal)<\/li>\n<li>lola Pe\u00f1arrubia blasco (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Jose P\u00e9rez G\u00f3mez Las giberelinas son sustancias reguladoras del crecimiento en plantas, y est\u00e1n implicadas en el [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[16820],"tags":[29610,72779,46981,72778,72780,9513],"class_list":["post-24856","post","type-post","status-publish","format-standard","hentry","category-politecnica-de-valencia","tag-blazquez-rodriguez-miguel-angel","tag-jaime-Martinez-garcia","tag-jose-luis-garcia-Martinez","tag-jose-perez-gomez","tag-lola-penarrubia-blasco","tag-ricardo-flores-pedauye"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/24856","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=24856"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/24856\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=24856"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=24856"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=24856"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}