{"id":25078,"date":"2018-03-09T09:16:19","date_gmt":"2018-03-09T09:16:19","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/inmovilizacion-de-ligandos-enzimas-y-anta%c2%adgenos-recombinantes-en-la-pared-celular-de-saccharomyces-cerevisiae-mediante-tecnicas-de-ingenieria-genetica\/"},"modified":"2018-03-09T09:16:19","modified_gmt":"2018-03-09T09:16:19","slug":"inmovilizacion-de-ligandos-enzimas-y-anta%c2%adgenos-recombinantes-en-la-pared-celular-de-saccharomyces-cerevisiae-mediante-tecnicas-de-ingenieria-genetica","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/inmovilizacion-de-ligandos-enzimas-y-anta%c2%adgenos-recombinantes-en-la-pared-celular-de-saccharomyces-cerevisiae-mediante-tecnicas-de-ingenieria-genetica\/","title":{"rendered":"Inmovilizaci\u00f3n de ligandos, enzimas y ant\u00edgenos recombinantes en la pared celular de saccharomyces cerevisiae mediante t\u00e9cnicas de ingenier\u00eda gen\u00e9tica"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Isabel Andres Borderia <\/strong><\/h2>\n<p>Saccharomyces cerevisiae es la levadura del pan, de la cerveza y del vino y en tal papel se ha venido utilizando desde las primeras sociedades agr\u00edcolas. en los \u00faltimos a\u00f1os, y gracias al desarrollo de la ingenier\u00eda gen\u00e9tica, s.Crevisiae ha a\u00f1adido a sus usos tradicionales el de servir de hospedador para la producci\u00f3n de prote\u00ednas heter\u00f3logas.  las c\u00e9lulas de s.Cerevisiae estan rodeadas por la pared celular, en cuya composici\u00f3n destacan las manoprote\u00ednas, las cuales deben necesariamente ser secretadas para llegar a su destino final, por lo que contienen tanto secuencias que determinan su secreci\u00f3n, as\u00ed como secuencias que determinan su retenci\u00f3n en la pared celular, y que vienen determinadas por el extremo carboxi-terminal de dichas prote\u00ednas. Estas secuencias pueden ser utilizadas para dirigir la localizaci\u00f3n de prote\u00ednas heter\u00f3logas a la pared celular, premisa en la que se basa este trabajo, el cual consisti\u00f3 en utilizar las se\u00f1ales de secreci\u00f3n y de retenci\u00f3n a la pared celular de dos prote\u00ednas caracterizadas anteriormente por nuestro grupo, pir4, una prote\u00edna extra\u00edble por agentes reductores, e icwp, una prote\u00edna gpi, para determinar la secreci\u00f3n, o en su caso la localizaci\u00f3n en la pared celular de distintas prote\u00ednas recombinantes con aplicaci\u00f3n en biotecnolog\u00eda, tal como un ligando espec\u00edfico que permitiese la inmovilizaci\u00f3n de las c\u00e9lulas en una matriz s\u00f3lida, una enzima activa o un ant\u00edgeno de un microorganismo pat\u00f3geno, aproximaciones que han dado lugar a los tres cap\u00edtulos de que consta esta tesis:  1,- inmovilizaci\u00f3n de c\u00e9lulas de saccharomyces cerevisiae en una matriz de prote\u00edna g.Sefarosa mediante ingenier\u00eda gen\u00e9tica de la pared celular.  2,- expresi\u00f3n de la xilanasa a de bacillus sp. Bp-7 en saccharomyces cerevisiae en forma de prote\u00edna de fusi\u00f3n con la prote\u00edna de la pared celular pir4.  3,- expresi\u00f3n de un ant\u00edgeno viral de la c\u00e1psida del rotavirus sa11 en saccharomyces<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Inmovilizaci\u00f3n de ligandos, enzimas y ant\u00edgenos recombinantes en la pared celular de saccharomyces cerevisiae mediante t\u00e9cnicas de ingenier\u00eda gen\u00e9tica<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Inmovilizaci\u00f3n de ligandos, enzimas y ant\u00edgenos recombinantes en la pared celular de saccharomyces cerevisiae mediante t\u00e9cnicas de ingenier\u00eda gen\u00e9tica <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Isabel Andres Borderia <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Universitat de val\u00e9ncia (estudi general)<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 25\/07\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Jes\u00fas Zueco Cruz<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: salvador Momeneo bernat <\/li>\n<li>palma Parascandola (vocal)<\/li>\n<li>Francisco ignacio Javier Pastor blasco (vocal)<\/li>\n<li>de Alteris elisabetta (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Isabel Andres Borderia Saccharomyces cerevisiae es la levadura del pan, de la cerveza y del vino y [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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