{"id":25110,"date":"2018-03-09T09:16:21","date_gmt":"2018-03-09T09:16:21","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/nuevos-inhibidores-del-intercambiador-na-h-accion-de-la-halocina-h6-y-del-peptido-inhibidor-pinhe-en-celulas-de-mamifero\/"},"modified":"2018-03-09T09:16:21","modified_gmt":"2018-03-09T09:16:21","slug":"nuevos-inhibidores-del-intercambiador-na-h-accion-de-la-halocina-h6-y-del-peptido-inhibidor-pinhe-en-celulas-de-mamifero","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/nuevos-inhibidores-del-intercambiador-na-h-accion-de-la-halocina-h6-y-del-peptido-inhibidor-pinhe-en-celulas-de-mamifero\/","title":{"rendered":"Nuevos inhibidores del intercambiador na+\/h+: acci\u00f3n de la halocina h6 y del peptido inhibidor pinhe en c\u00e9lulas de mamifero"},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Dolz Izquierdo M. Carmen <\/strong><\/h2>\n<p>A partir del sobrenadante del cultivo de la haloarquea haloferax gibbonsii ma 23.39 se obtiene la halocina h6 que inhibe el intercambiador na+\/h+ de haloarqueas sensibles. Este intercambiador es vital para estas c\u00e9lulas que viven en ambientes hipersalinos y lo precisan para mantener la homeostasis del na+. Una purificaci\u00f3n del sobrenadante del citado cultivo de h.Gibbonssi sobre la base de la precipitaci\u00f3n con acetona y fplc en fase reversa con gradiente de acetonitrio permite purificar un p\u00e9ptido que conserva toda la actividad de h6. Este p\u00e9ptido, pinhe, tiene un peso molecular de 2732 da y est\u00e1 formado por 28 amino\u00e1cidos. Se trata de un p\u00e9ptido hidr\u00f3fobo y termorresistente, soporta rangos amplios de ph, su actividad es independiente de la concentraci\u00f3n salina y es sensible a pronasa y resistente a tripsina. sobre la base del objetivo principal del presente trabajo de determinar si tanto h6 como pinhe inhiben elintercambiador nhe de eucariotas se desarroll\u00f3 un modelo de ensayo para determinar la actividad de nhe. Dicho modelo se bas\u00f3 en producir una acidificaci\u00f3n del citoplasma de c\u00e9lulas de mam\u00edferos conpulsos de \u00e1cido propi\u00f3nico y seguir la evoluci\u00f3n del phi (intracelular) por citometr\u00eda de flujo. Se bloquearon otros transportadores implicados en la regulaci\u00f3n de phi (ncs y ndae). La actividad de nhe se calcul\u00f3 sobre la base de determinar la velocidad inicial de recuperaci\u00f3n de phi a partir del ajuste matem\u00e1tico de los datos experimentales. Todas la c\u00e9lulas utilizadas: fibroblastos de rat\u00f3n nih3t3, embrionarias de ri\u00f1\u00f3n humano hek293, cardiomiocitos de rat\u00f3n hl-1 y cultivos primarios de m\u00fasculo esquel\u00e9tico humano respondieron al modelo experimental propuesto de forma similar. Tanto h6 como pinhe inhiben el nhe de c\u00e9lulas de mam\u00edfero. Pinhe inhibe de forma espec\u00edfica y reversible el nhe. Esta inhibici\u00f3n es dependiente de la concentraci\u00f3n de pinhe pudiendo calcularse una concentraci\u00f3n inhibitoria del 50%<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Nuevos inhibidores del intercambiador na+\/h+: acci\u00f3n de la halocina h6 y del peptido inhibidor pinhe en c\u00e9lulas de mamifero<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Nuevos inhibidores del intercambiador na+\/h+: acci\u00f3n de la halocina h6 y del peptido inhibidor pinhe en c\u00e9lulas de mamifero <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Dolz Izquierdo M. Carmen <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Universitat de val\u00e9ncia (estudi general)<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 28\/07\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li> Lequerica Llopis Juan  Luis<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: lu\u00eds Such belenguer <\/li>\n<li>Jos\u00e9 Montero argudo (vocal)<\/li>\n<li>inmaculada Meseguer soria (vocal)<\/li>\n<li>lisardo Bosc\u00e1 gomar (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Dolz Izquierdo M. Carmen A partir del sobrenadante del cultivo de la haloarquea haloferax gibbonsii ma 23.39 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[1],"tags":[73379,70175,73381,73380,5664,11418],"class_list":["post-25110","post","type-post","status-publish","format-standard","hentry","category-sin-categoria","tag-dolz-izquierdo-m-carmen","tag-inmaculada-meseguer-soria","tag-jose-montero-argudo","tag-lequerica-llopis-juan-luis","tag-lisardo-bosca-gomar","tag-luis-such-belenguer"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/25110","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=25110"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/25110\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=25110"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=25110"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=25110"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}