{"id":25452,"date":"2018-03-09T09:16:51","date_gmt":"2018-03-09T09:16:51","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/desarrollo-de-metodos-inmunocromatograficos-para-la-determinacion-de-trazas-de-b-lactoglobulina\/"},"modified":"2018-03-09T09:16:51","modified_gmt":"2018-03-09T09:16:51","slug":"desarrollo-de-metodos-inmunocromatograficos-para-la-determinacion-de-trazas-de-b-lactoglobulina","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/alcala\/desarrollo-de-metodos-inmunocromatograficos-para-la-determinacion-de-trazas-de-b-lactoglobulina\/","title":{"rendered":"Desarrollo de metodos inmunocromatograficos para la determinacion de trazas de b lactoglobulina."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Angel De La Puerta Garcia Barroso <\/strong><\/h2>\n<p>La b-lactoglobulina (blg) es el principal alergeno presente en leche de vaca. Para evitar las alergias provocadas por esta prote\u00edna se han comercializado f\u00f3rmulas hipoalerg\u00e9nicas, en las que se ha intentado eliminar este alergeno. sin embargo, se han descrito casos de alergia tras la ingesti\u00f3n de estos alimentos, que hacen pensar en la existencia de cantidades residuales de la prote\u00edna o de sus fragmentos pept\u00eddicos.  para realizar la determinaci\u00f3n de este alergeno en concentraciones tan bajas en matrices complejas como son los alimentos es necesario disponer de un m\u00e9todo de an\u00e1lisis de blg altamente selectivo y sensible, con elevada precisi\u00f3n, r\u00e1pido y automatizable, condiciones que cumplen los inmunoensayos en sistemas cromatogr\u00e1ficos.  por tanto, se ha llevado a cabo el desarrollo de un m\u00e9todo inmunocromatogr\u00e1fico empleando como relleno cromatogr\u00e1fico un soporte con anticuerpos espec\u00edficos antiblg para la cuantificaci\u00f3n a niveles traza de blg en f\u00f3rmulas hipoalerg\u00e9nicas basadas en prote\u00ednas de leche de vaca. La inyecci\u00f3n del preparado hipoalerg\u00e9nico en la columna origina la retenci\u00f3n selectiva de blg y sus fragmentos pept\u00eddicos antig\u00e9nicos. Una etapa posterior de reconocimiento en la propia columna con un anticuerpo anti blg marcado con una sonda enzim\u00e1tica seguida de la inyecci\u00f3n de sustrato de la reacci\u00f3n enzim\u00e1tica origina una cantidad de producto proporcional a la cantidad de ant\u00edgeno existente en la muestra, que se cuantifica en el detector del sistema cromatogr\u00e1fico. El m\u00e9todo desarrollado se ha identificado con el nombre eliac (enzyme-linked immunoaffinity chromatography).  el estudio se ha estructurado del siguiente modo: 1) estudio de diversos procedimientos de uni\u00f3n covalente de antiblg a soportes cromatogr\u00e1ficos de diferentes caracter\u00edsticas, 2) selecci\u00f3n y evaluaci\u00f3n, mediante inmunodotting, de las soluciones a emplear durante el inmunoensayo en columna, 3) caracterizaci\u00f3n de las<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Desarrollo de metodos inmunocromatograficos para la determinacion de trazas de b lactoglobulina.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Desarrollo de metodos inmunocromatograficos para la determinacion de trazas de b lactoglobulina. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Angel De La Puerta Garcia Barroso <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Alcal\u00e1<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 17\/09\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li> Frutos Gomez Mercedes De<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Mar\u00eda  teresa Galceran huguet <\/li>\n<li>Elena Molina hernandez (vocal)<\/li>\n<li> De bolos pi carmen (vocal)<\/li>\n<li>carmen Guiberteau cabanillas (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Angel De La Puerta Garcia Barroso La b-lactoglobulina (blg) es el principal alergeno presente en leche de [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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