{"id":25881,"date":"2018-03-09T09:17:27","date_gmt":"2018-03-09T09:17:27","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/efectos-del-oxido-na%c2%adtrico-en-la-diferenciacion-de-monocitos-de-sangre-periferica-a-celulas-dendra%c2%adticas\/"},"modified":"2018-03-09T09:17:27","modified_gmt":"2018-03-09T09:17:27","slug":"efectos-del-oxido-na%c2%adtrico-en-la-diferenciacion-de-monocitos-de-sangre-periferica-a-celulas-dendra%c2%adticas","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/navarra\/efectos-del-oxido-na%c2%adtrico-en-la-diferenciacion-de-monocitos-de-sangre-periferica-a-celulas-dendra%c2%adticas\/","title":{"rendered":"Efectos del \u00f3xido n\u00edtrico en la diferenciaci\u00f3n de monocitos de sangre perif\u00e9rica a c\u00e9lulas dendr\u00edticas"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Ver\u00f3nica Fern\u00e1ndez Ruiz <\/strong><\/h2>\n<p>El objetivo de este trabajo es determinar el efecto del \u00f3xido n\u00edtrico (no) en la diferenciaci\u00f3n de monocitos a c\u00e9lulas dendr\u00edticas y en su posterior maduraci\u00f3n. Los monocitos humanos cd14+ cd1a- cd80- cd40- cd86+ hla-dr+, aislados de voluntarios sanos, se diferenciaron con gm-csf e il-4 a c\u00e9lulas dendr\u00edticas cd14- cd1a+ cd80+ cd40+ cd86+ hla-dr+. La adici\u00f3n de distintos donadores de no al comienzo de la diferenciaci\u00f3n produjo un incremento selectivo de la subpoblaci\u00f3n de c\u00e9lulas dendr\u00edticas cd80+ cd1a+ hla-dr+. las actividades arginasa ii y sintasa inducible de \u00f3xido n\u00edtrico (inos) se detectaron en los monocitos y en las c\u00e9lulas dendr\u00edticas. Los donadores sin-1 y deta-no aumentaron significativamente la actividad inos respecto a las c\u00e9lulas dendr\u00edticas control, aunque no se observaron cambios ni en la actividad arginasa ni en la expresi\u00f3n de arginasa ii. Los peroxinitritos mostraron un efecto t\u00f3xico superior al \u00f3xido n\u00edtrico y esta toxicidad result\u00f3 mayor en la diferenciaci\u00f3n de monocitos que en la maduraci\u00f3n de las c\u00e9lulas dendr\u00edticas. La adici\u00f3n de l-nmma el primer d\u00eda de la diferenciaci\u00f3n increment\u00f3 la actividad nos en las c\u00e9lulas dendr\u00edticas. El no produjo una intensa nitraci\u00f3n de prote\u00ednas; se detect\u00f3 una banda de 90 kda que aparece en c\u00e9lulas dendr\u00edticas y no en monocitos. Adem\u00e1s, todas las c\u00e9lulas cd1a+ y cd83+ mostraron una homog\u00e9nea e intensa nitraci\u00f3n de prote\u00ednas. La maduraci\u00f3n con gm-csf y tnf-alfa produjo la aparici\u00f3n de dos subpoblaciones; hla-dr++ cd83+ y hla-dr+ cd83-, la estimulaci\u00f3n con lps e ifn-gamma produjo una poblaci\u00f3n homog\u00e9nea hla-dr++ cd83+. Las actividades arginasa e inos se detectaron en las c\u00e9lulas dendr\u00edticas maduras. La adici\u00f3n de deta-no en la maduraci\u00f3n produjo un incremento significativo de la actividad inos, lo que podr\u00eda estar relacionado con una mayor expresi\u00f3n del cd83 en estas c\u00e9lulas. Los resultados sugieren una relaci\u00f3n entre el fenotipo, la pro<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Efectos del \u00f3xido n\u00edtrico en la diferenciaci\u00f3n de monocitos de sangre perif\u00e9rica a c\u00e9lulas dendr\u00edticas<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Efectos del \u00f3xido n\u00edtrico en la diferenciaci\u00f3n de monocitos de sangre perif\u00e9rica a c\u00e9lulas dendr\u00edticas <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Ver\u00f3nica Fern\u00e1ndez Ruiz <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Navarra<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 26\/09\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Alvaro Gonzalez Hernandez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal:  Escanero marcen Jes\u00fas fernando <\/li>\n<li>concepci\u00f3n Gonz\u00e1lez rodr\u00edguez (vocal)<\/li>\n<li> Gonz\u00e1lez de buitrago arriero Jos\u00e9 Manuel (vocal)<\/li>\n<li>laura Guembe echarri (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Ver\u00f3nica Fern\u00e1ndez Ruiz El objetivo de este trabajo es determinar el efecto del \u00f3xido n\u00edtrico (no) en [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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