{"id":25889,"date":"2018-03-09T09:17:28","date_gmt":"2018-03-09T09:17:28","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/purificacion-y-caracterizacion-genetica-y-molecular-de-lipasas-de-candida-rugosa\/"},"modified":"2018-03-09T09:17:28","modified_gmt":"2018-03-09T09:17:28","slug":"purificacion-y-caracterizacion-genetica-y-molecular-de-lipasas-de-candida-rugosa","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/vigo\/purificacion-y-caracterizacion-genetica-y-molecular-de-lipasas-de-candida-rugosa\/","title":{"rendered":"Purificaci\u00f3n y caracterizaci\u00f3n gen\u00e9tica y molecular de lipasas de candida rugosa"},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Pernas Carralero M. Antonia <\/strong><\/h2>\n<p>Las lipasas son una familia de enzimas que catalizan la hidr\u00f3lisis de triglic\u00e9ridos con diferentes tipos de especificidad (y capacidad para reacciones de s\u00edntesis), lo que les confiere, especialmente a las de origen microbiano, un potencial industrial en los sectores alimentarios y farmac\u00e9utico. Candida rugosa posee al menos 7 genes que codifican (con alguna excepci\u00f3n al c\u00f3digo gen\u00e9tico universal) otras tantas lipasas cuya purificaci\u00f3n, caracterizaci\u00f3n y cristalizaci\u00f3n no ha sido posible en todas ellas. Precisamente, los costes de producci\u00f3n y la disponibilidad de formas puras ha limitado hasta ahora la extensi\u00f3n de los usos y la comprensi\u00f3n de las relaciones estructura-actividad. El presente trabajo que comprende la purificaci\u00f3n y caracterizaci\u00f3n (bioqu\u00edmica y cin\u00e9tica) de las lipasas de candida rugosa presentes tanto en un extracto comercial como en un postincubado producido ad hoc, forma parte d eunproyecto m\u00e1s amplio que integra estos aspectos junto con los de producci\u00f3n a escala piloto y aplicaci\u00f3n en reacciones de qu\u00edmica fina.  de este modo, se ha resuelto, partiendo de las fuentes indicadas y proponiendo diferentes alternativas operatorias, la purificaci\u00f3n de las lipasas lip1, lip2 y lip3 (esta \u00faltima en forma de mon\u00f3mero y d\u00edmero) en cantidades suficientes como para permitir comparar de forma sistem\u00e1tica sus propiedades y, en el caso particular de lip2, su cristalizaci\u00f3n. As\u00ed, a pesar de su similitud en cuanto a valores de peso molecular, punto isoel\u00e9ctrico y grado de glicosilaci\u00f3n, las enzimas mostraron diferencias en la especificidad de sustrato, especialmente significativas frente a la longitud de cadena de triglic\u00e9ridos y en la estabilidad frente al efecto combinado del ph y la temperatura, siendo las m\u00e1s termoestables lip2 y lip3 dim\u00e9rica.  tambi\u00e9n el comportamiento cin\u00e9tico fue diferente. Mientras lip3 dim\u00e9rica mostr\u00f3 perfiles pr\u00e1cticamente michaelianos como consecuencias de la mayor<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Purificaci\u00f3n y caracterizaci\u00f3n gen\u00e9tica y molecular de lipasas de candida rugosa<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Purificaci\u00f3n y caracterizaci\u00f3n gen\u00e9tica y molecular de lipasas de candida rugosa <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Pernas Carralero M. Antonia <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Vigo<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 26\/09\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li> Rua Rodr\u00edguez M. Luisa<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jos\u00e9 Mar\u00eda Teijon rivera <\/li>\n<li>teresa D\u00edaz Garc\u00eda-mauri\u00f1o (vocal)<\/li>\n<li> S\u00e1nchez montero Jos\u00e9 Mar\u00eda (vocal)<\/li>\n<li>Jos\u00e9 Berenguer Carlos (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Pernas Carralero M. Antonia Las lipasas son una familia de enzimas que catalizan la hidr\u00f3lisis de triglic\u00e9ridos [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[18657],"tags":[6063,13528,75187,75188,75190,75189],"class_list":["post-25889","post","type-post","status-publish","format-standard","hentry","category-vigo","tag-jose-berenguer-carlos","tag-jose-maria-teijon-rivera","tag-pernas-carralero-m-antonia","tag-rua-rodriguez-m-luisa","tag-sanchez-montero-jose-maria","tag-teresa-diaz-garcia-maurino"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/25889","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=25889"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/25889\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=25889"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=25889"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=25889"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}