{"id":26286,"date":"2018-03-09T09:18:02","date_gmt":"2018-03-09T09:18:02","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/efecto-de-la-hipoxia-sobre-la-expresion-de-la-sintasa-inducible-de-oxido-na%c2%adtrico-y-la-adrenomedulina-en-modelos-in-vivo-e-in-vitro-de-la-lesion-aguda-de-pulmon\/"},"modified":"2018-03-09T09:18:02","modified_gmt":"2018-03-09T09:18:02","slug":"efecto-de-la-hipoxia-sobre-la-expresion-de-la-sintasa-inducible-de-oxido-na%c2%adtrico-y-la-adrenomedulina-en-modelos-in-vivo-e-in-vitro-de-la-lesion-aguda-de-pulmon","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/navarra\/efecto-de-la-hipoxia-sobre-la-expresion-de-la-sintasa-inducible-de-oxido-na%c2%adtrico-y-la-adrenomedulina-en-modelos-in-vivo-e-in-vitro-de-la-lesion-aguda-de-pulmon\/","title":{"rendered":"Efecto de la hipoxia sobre la expresi\u00f3n de la sintasa inducible de \u00f3xido n\u00edtrico y la adrenomedulina en modelos in vivo e in vitro de la lesi\u00f3n aguda de pulm\u00f3n"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Jackeline Agorreta Arrazubi <\/strong><\/h2>\n<p>El tratamniento con lipopolisac\u00e1rido (lps) reproduce algunos de los s\u00edntomas de la lesi\u00f3n aguda de pulm\u00f3n (ali), y la hipoxemia es una de las principales caracter\u00edsticas de esta enfermedad; por lo que hemos desarrollado un modelo de lesi\u00f3n aguda de pulm\u00f3n que combina los tratamientos de hipoxia normob\u00e1rica aguda y lps en pulm\u00f3n de rata (modelo in vivo) y en distintas l\u00edneas celulares establecidas (modelo in vitro). En ambos modelos se combinan el tratamiento de lps (0,1 mg\/kg, i.P en el modelo animal y 100 ng\/ml en las l\u00edneas celulares) e hipoxia aguda (9% o2 en el modelo animal y 1% o2 en las l\u00ednes celulares) en ratas fisher harlan y l\u00edneas celulares procedentes de pulm\u00f3n de rata. se analizaron cuatro grupos de estudio: grupo 1 (normoxia), grupo 2 (hipoxia), grupo 3 (normoxia + lps), grupo 4 (hipoxia +lps).  varios autores han descrito un aumento de la expresi\u00f3n de la sintasa inducible de \u00f3xido n\u00edtrico nos2)j y la adrenomedulina (am) en pacientes con ali, y ambos factores son regulados por los tratamientos con hipoxia y lps. se ha estudiado la expresi\u00f3n de mabos factores en los modelos in vivo e in vitro aplicando las t\u00e9cnicas de northern blot, western blot, hibridaci\u00f3n in situ de mrna, inmunocitoqu\u00edmica y radioinmunoensayo para detectar la producci\u00f3n nos2 y am. Se ha obervado que el tratamiento con hipoxia + lps produce edema pulmonar y una acumulaci\u00f3n de mol\u00e9culas extravasadas en la pared de los vasos sangu\u00edneos, lo qu epareceindicar que este tratamiento reproduce las fases tempras de ali. En el modelo in vivo el tratamiento combinado produce una potenciaci\u00f3n de la expresi\u00f3n de nos2 y am en comparaci\u00f3n con ambos tratamientos por separado. En el caso de nos2, este aumento de la expresi\u00f3n es debido a una inducci\u00f3n de la migraci\u00f3n al pulm\u00f3n  de c\u00e9lulas inflamatorias inmunorreactivas para nos2 identificadas como monocitos, neutr\u00f3filos y linfocitos. En el caso de la am, s\u00f3lo la l\u00ednea celular de macr\u00f3fagos<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Efecto de la hipoxia sobre la expresi\u00f3n de la sintasa inducible de \u00f3xido n\u00edtrico y la adrenomedulina en modelos in vivo e in vitro de la lesi\u00f3n aguda de pulm\u00f3n<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Efecto de la hipoxia sobre la expresi\u00f3n de la sintasa inducible de \u00f3xido n\u00edtrico y la adrenomedulina en modelos in vivo e in vitro de la lesi\u00f3n aguda de pulm\u00f3n <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Jackeline Agorreta Arrazubi <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Navarra<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 14\/10\/2003<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Mar\u00eda Mercedes Garayoa Berrueta<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: jose Rodrigo garcia <\/li>\n<li>Francisco Jos\u00e9 Esteban ruiz (vocal)<\/li>\n<li>joan albert Barber\u00e1 mir (vocal)<\/li>\n<li>rub\u00e9n Pio os\u00e9s (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Jackeline Agorreta Arrazubi El tratamniento con lipopolisac\u00e1rido (lps) reproduce algunos de los s\u00edntomas de la lesi\u00f3n aguda [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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